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Malaria Journal
Published in: Malaria Journal 1/2015

Open Access 01-12-2015 | Research

Genetic determinants of anti-malarial acquired immunity in a large multi-centre study

Authors: Jennifer M G Shelton, Patrick Corran, Paul Risley, Nilupa Silva, Christina Hubbart, Anna Jeffreys, Kate Rowlands, Rachel Craik, Victoria Cornelius, Meike Hensmann, Sile Molloy, Nuno Sepulveda, Taane G Clark, Gavin Band, Geraldine M Clarke, Christopher C A Spencer, Angeliki Kerasidou, Susana Campino, Sarah Auburn, Adama Tall, Alioune Badara Ly, Odile Mercereau-Puijalon, Anavaj Sakuntabhai, Abdoulaye Djimdé, Boubacar Maiga, Ousmane Touré, Ogobara K Doumbo, Amagana Dolo, Marita Troye-Blomberg, Valentina D Mangano, Frederica Verra, David Modiano, Edith Bougouma, Sodiomon B Sirima, Muntaser Ibrahim, Ayman Hussain, Nahid Eid, Abier Elzein, Hiba Mohammed, Ahmed Elhassan, Ibrahim Elhassan, Thomas N Williams, Carolyne Ndila, Alexander Macharia, Kevin Marsh, Alphaxard Manjurano, Hugh Reyburn, Martha Lemnge, Deus Ishengoma, Richard Carter, Nadira Karunaweera, Deepika Fernando, Rajika Dewasurendra, Christopher J Drakeley, Eleanor M Riley, Dominic P Kwiatkowski, Kirk A Rockett, In collaboration with the MalariaGEN Consortium

Published in: Malaria Journal | Issue 1/2015

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Abstract

Background

Many studies report associations between human genetic factors and immunity to malaria but few have been reliably replicated. These studies are usually country-specific, use small sample sizes and are not directly comparable due to differences in methodologies. This study brings together samples and data collected from multiple sites across Africa and Asia to use standardized methods to look for consistent genetic effects on anti-malarial antibody levels.

Methods

Sera, DNA samples and clinical data were collected from 13,299 individuals from ten sites in Senegal, Mali, Burkina Faso, Sudan, Kenya, Tanzania, and Sri Lanka using standardized methods. DNA was extracted and typed for 202 Single Nucleotide Polymorphisms with known associations to malaria or antibody production, and antibody levels to four clinical grade malarial antigens [AMA1, MSP1, MSP2, and (NANP)4] plus total IgE were measured by ELISA techniques. Regression models were used to investigate the associations of clinical and genetic factors with antibody levels.

Results

Malaria infection increased levels of antibodies to malaria antigens and, as expected, stable predictors of anti-malarial antibody levels included age, seasonality, location, and ethnicity. Correlations between antibodies to blood-stage antigens AMA1, MSP1 and MSP2 were higher between themselves than with antibodies to the (NANP)4 epitope of the pre-erythrocytic circumsporozoite protein, while there was little or no correlation with total IgE levels. Individuals with sickle cell trait had significantly lower antibody levels to all blood-stage antigens, and recessive homozygotes for CD36 (rs321198) had significantly lower anti-malarial antibody levels to MSP2.

Conclusion

Although the most significant finding with a consistent effect across sites was for sickle cell trait, its effect is likely to be via reducing a microscopically positive parasitaemia rather than directly on antibody levels. However, this study does demonstrate a framework for the feasibility of combining data from sites with heterogeneous malaria transmission levels across Africa and Asia with which to explore genetic effects on anti-malarial immunity.
Appendix
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Metadata
Title
Genetic determinants of anti-malarial acquired immunity in a large multi-centre study
Authors
Jennifer M G Shelton
Patrick Corran
Paul Risley
Nilupa Silva
Christina Hubbart
Anna Jeffreys
Kate Rowlands
Rachel Craik
Victoria Cornelius
Meike Hensmann
Sile Molloy
Nuno Sepulveda
Taane G Clark
Gavin Band
Geraldine M Clarke
Christopher C A Spencer
Angeliki Kerasidou
Susana Campino
Sarah Auburn
Adama Tall
Alioune Badara Ly
Odile Mercereau-Puijalon
Anavaj Sakuntabhai
Abdoulaye Djimdé
Boubacar Maiga
Ousmane Touré
Ogobara K Doumbo
Amagana Dolo
Marita Troye-Blomberg
Valentina D Mangano
Frederica Verra
David Modiano
Edith Bougouma
Sodiomon B Sirima
Muntaser Ibrahim
Ayman Hussain
Nahid Eid
Abier Elzein
Hiba Mohammed
Ahmed Elhassan
Ibrahim Elhassan
Thomas N Williams
Carolyne Ndila
Alexander Macharia
Kevin Marsh
Alphaxard Manjurano
Hugh Reyburn
Martha Lemnge
Deus Ishengoma
Richard Carter
Nadira Karunaweera
Deepika Fernando
Rajika Dewasurendra
Christopher J Drakeley
Eleanor M Riley
Dominic P Kwiatkowski
Kirk A Rockett
In collaboration with the MalariaGEN Consortium
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2015
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-015-0833-x

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