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Cancer Cell International
Published in: Cancer Cell International 1/2019

Open Access 01-12-2019 | Colorectal Cancer | Primary research

TRIM52 promotes colorectal cancer cell proliferation through the STAT3 signaling

Authors: Shengli Pan, Yingying Deng, Jun Fu, Yuhao Zhang, Zhijin Zhang, Xiaokun Ru, Xianju Qin

Published in: Cancer Cell International | Issue 1/2019

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Abstract

Background

The tripartite motif (TRIM) family proteins are implicated in the pathogenesis of various human malignancies. The up-regulation and oncogenic roles of TRIM52 have been reported in hepatocellular carcinoma. In the current study, we aimed to examine its expression and possible function in colorectal cancer (CRC).

Method

Immunohistochemical staining or immunoblotting analysis was carried out to detect protein expression. Cell proliferation and apoptosis was evaluated by Cell Counting Kit-8 (CCK-8) and flow cytometry assay, respectively.

Results

TRIM52 expression was increased in 67.5% of CRC tissues (54/80) compared to matched normal colonic mucosa. TRIM52 expression was closely related with tumor size (p = 0.0376), tumor stage (p = 0.0227) and overall survival (p = 0.0177). Short hairpin RNAs (shRNAs) targeting TRIM52 had the potential anti-proliferative effects on CRC cell lines, SW480 and LoVo, by inducing cell apoptosis. In addition, an in vivo xenograft experiment confirmed the in vitro results. In addition, TRIM52 shRNAs decreased the phosphorylation of STAT3, but increased the protein expression of SHP2, a negative regulator of STAT3 phosphorylation. TRIM52 formed a complex with SHP2 and promoted the ubiquitination of SHP2. Furthermore, inhibition of the STAT3 signaling by AG490 in RKO cells significantly abolished the effects of TRIM52 overexpression on cell proliferation, apoptosis and STAT3 activation.

Conclusions

TRIM52 might exert oncogenic role in CRC via regulating the STAT3 signaling pathway.
Appendix
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Metadata
Title
TRIM52 promotes colorectal cancer cell proliferation through the STAT3 signaling
Authors
Shengli Pan
Yingying Deng
Jun Fu
Yuhao Zhang
Zhijin Zhang
Xiaokun Ru
Xianju Qin
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2019
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-019-0775-4

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