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Respiratory Research
Published in: Respiratory Research 1/2020

01-12-2020 | Respiratory Microbiota | Research

Differences in airway microbiome and metabolome of single lung transplant recipients

Authors: Nirmal S. Sharma, Grant Vestal, Keith Wille, Kapil N. Patel, Feng Cheng, Srinivas Tipparaju, Sultan Tousif, Mudassir M. Banday, Xin Xu, Landon Wilson, Viswam S. Nair, Casey Morrow, Don Hayes Jr, Andreas Seyfang, Stephen Barnes, Jessy S. Deshane, Amit Gaggar

Published in: Respiratory Research | Issue 1/2020

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Abstract

Background

Recent studies suggest that alterations in lung microbiome are associated with occurrence of chronic lung diseases and transplant rejection. To investigate the host-microbiome interactions, we characterized the airway microbiome and metabolome of the allograft (transplanted lung) and native lung of single lung transplant recipients.

Methods

BAL was collected from the allograft and native lungs of SLTs and healthy controls. 16S rRNA microbiome analysis was performed on BAL bacterial pellets and supernatant used for metabolome, cytokines and acetylated proline-glycine-proline (Ac-PGP) measurement by liquid chromatography-high-resolution mass spectrometry.

Results

In our cohort, the allograft airway microbiome was distinct with a significantly higher bacterial burden and relative abundance of genera Acinetobacter & Pseudomonas. Likewise, the expression of the pro-inflammatory cytokine VEGF and the neutrophil chemoattractant matrikine Ac-PGP in the allograft was significantly higher. Airway metabolome distinguished the native lung from the allografts and an increased concentration of sphingosine-like metabolites that negatively correlated with abundance of bacteria from phyla Proteobacteria.

Conclusions

Allograft lungs have a distinct microbiome signature, a higher bacterial biomass and an increased Ac-PGP compared to the native lungs in SLTs compared to the native lungs in SLTs. Airway metabolome distinguishes the allografts from native lungs and is associated with distinct microbial communities, suggesting a functional relationship between the local microbiome and metabolome.
Appendix
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Metadata
Title
Differences in airway microbiome and metabolome of single lung transplant recipients
Authors
Nirmal S. Sharma
Grant Vestal
Keith Wille
Kapil N. Patel
Feng Cheng
Srinivas Tipparaju
Sultan Tousif
Mudassir M. Banday
Xin Xu
Landon Wilson
Viswam S. Nair
Casey Morrow
Don Hayes Jr
Andreas Seyfang
Stephen Barnes
Jessy S. Deshane
Amit Gaggar
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2020
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/s12931-020-01367-3

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