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Respiratory Research
Published in: Respiratory Research 1/2017

Open Access 01-12-2017 | Research

Exposure to ambient particulate matter alters the microbial composition and induces immune changes in rat lung

Authors: Naijian Li, Fang He, Baoling Liao, Yuming Zhou, Bing Li, Pixin Ran

Published in: Respiratory Research | Issue 1/2017

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Abstract

Background

Ambient particulate matter exposure has been shown to increase the risks of respiratory diseases. However, the role of the lung microbiome and the immune response to inhaled particulate matter are largely unexplored. We studied the influence of biomass fuel and motor vehicle exhaust particles on the lung microbiome and pulmonary immunologic homeostasis in rats.

Methods

Fifty-seven Sprague–Dawley rats were randomly divided into clean air (CON), biomass fuel (BMF), and motor vehicle exhaust (MVE) groups. After a 4-week exposure, the microbial composition of the lung was assessed by 16S rRNA pyrosequencing, the structure of the lung tissue was assessed with histological analysis, the phagocytic response of alveolar macrophages to bacteria was determined by flow cytometry, and immunoglobulin concentrations were measured with commercial ELISA kits.

Results

There was no significant difference in lung morphology between the groups. However, the BMF and MVE groups displayed greater bacterial abundance and diversity. Proteobacteria were present in higher proportions in the MVE group, and 12 bacterial families differed in their relative abundances between the three groups. In addition, particulate matter exposure significantly increased the capacity of alveolar macrophages to phagocytose bacteria and induced changes in immunoglobulin levels.

Conclusion

We demonstrated that particulate matter exposure can alter the microbial composition and change the pulmonary immunologic homeostasis in the rat lung.
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Metadata
Title
Exposure to ambient particulate matter alters the microbial composition and induces immune changes in rat lung
Authors
Naijian Li
Fang He
Baoling Liao
Yuming Zhou
Bing Li
Pixin Ran
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2017
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/s12931-017-0626-6

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