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Open Access 01-12-2017 | Research

Exogenous gene transfer of Rab³8 small GTPase ameliorates aberrant lung surfactant homeostasis in Ruby rats

Authors: Kazuhiro Osanai, Keisuke Nakase, Takashi Sakuma, Kazuaki Nishiki, Masafumi Nojiri, Ryo Kato, Masatoshi Saito, Yuki Fujimoto, Shiro Mizuno, Hirohisa Toga

Published in: Respiratory Research | Issue 1/2017

Abstract

Background

Rab³8 small GTPase regulates intracellular transport in melanocytes and alveolar type II epithelial cells. Ruby rats carrying Rab³8 and other gene mutations exhibit oculocutaneous albinism, bleeding diathesis, and hence, are a rat model of human Hermansky-Pudlak syndrome (HPS). We previously showed that Long Evans Cinnamon (LEC) rats, one strain of the Ruby rats, developed aberrant lung surfactant homeostasis with remarkably enlarged lamellar bodies in alveolar type II cells.

Methods

A replication-deficient recombinant adenovirus expressing rat Rab³8 (Ad-Rab³8) was constructed. Alveolar type II cells were isolated from the LEC rats and tested for lung surfactant phosphatidylcholine secretion. The rats were also examined whether exogenous expression of Ad- Rab³8 could rescue the altered lung surfactant homeostasis in the lungs.

Results

Isolated type II cells infected with Ad-Rab³8 exhibited improved secretion patterns of [³H]phosphatidylcholine, i.e. increased basal hyposecretion and decreased agonist-induced hypersecretion. Endobronchial administration of Ad-Rab³8 improved the morphology of type II cells and lamellar bodies, reducing their sizes close to those of wild-type rats. The increased amounts of phosphatidylcholine and surfactant protein B in the lamellar body fractions were decreased in the Ad-Rab³8 infected lungs.

Conclusions

These results provide strong evidence that the aberrant lung surfactant homeostasis in the LEC rats is caused by Rab³8 deficit, and suggest that endobronchial delivery of the responsive transgene could be an effective method to ameliorate the abnormal lung phenotype in the animal model of HPS.

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Title: Exogenous gene transfer of Rab38 small GTPase ameliorates aberrant lung surfactant homeostasis in Ruby rats
Authors: Kazuhiro Osanai
Keisuke Nakase
Takashi Sakuma
Kazuaki Nishiki
Masafumi Nojiri
Ryo Kato
Masatoshi Saito
Yuki Fujimoto
Shiro Mizuno
Hirohisa Toga
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Respiratory Research / Issue 1/2017
Electronic ISSN: 1465-993X
DOI: https://doi.org/10.1186/s12931-017-0549-2

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Exogenous gene transfer of Rab³8 small GTPase ameliorates aberrant lung surfactant homeostasis in Ruby rats

Abstract

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Methods

Results

Conclusions

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