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Respiratory Research

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Open Access 01-12-2015 | Research

Autophagy contributes to the chemo-resistance of non-small cell lung cancer in hypoxic conditions

Authors: Jin Gu Lee, Ju Hye Shin, Hyo Sup Shim, Chang Young Lee, Dae Joon Kim, Young Sam Kim, Kyung Young Chung

Published in: Respiratory Research | Issue 1/2015

Abstract

Background

The development of chemo-resistance in non-small lung cancer is a major obstacle in treating patients. Hypoxia is a commonly faced microenvironment in solid tumor and suggested to be related to both autophagy and chemo-resistance.

Methods

In this study, we investigated the role of hypoxia-induced autophagy in acquiring chemo-resistance in both cancer cell (A549) and human cancer tissue

Results

Hypoxic exposure (1 % O2) of A549 cell stimulated autophagic induction in cancer cells, shown by increase of LC3BI to LC3BII conversion and decrease of p62/sequestosome1 in Western blot, increased GFP-LC puncta in confocal microscopy, and increased number of double-membrane autophagic vacuoles in electron micrographs. Hypoxic exposure also induced resistance of cancer cells to cisplatin, and LC3B siRNA restored the sensitivity of cancer cells to chemotherapy. Furthermore, Human lung cancer tissues that experienced chemotherapy showed increase of LC3BI to LC3BII conversion and decrease of p62/sequestosome1 compared with chemo-naïve cancer tissue in Western blot.

Conclusion

Autophagy may play an important role in acquiring resistance to chemotherapy in lung cancer and hypoxia related pathway seems to be involved in autophagy induction.

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Title: Autophagy contributes to the chemo-resistance of non-small cell lung cancer in hypoxic conditions
Authors: Jin Gu Lee
Ju Hye Shin
Hyo Sup Shim
Chang Young Lee
Dae Joon Kim
Young Sam Kim
Kyung Young Chung
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Respiratory Research / Issue 1/2015
Electronic ISSN: 1465-993X
DOI: https://doi.org/10.1186/s12931-015-0285-4

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Autophagy contributes to the chemo-resistance of non-small cell lung cancer in hypoxic conditions

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Results

Conclusion

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