Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2019

Open Access 01-12-2019 | Research article

Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells

Authors: Xiaofeng Shi, Ruiqin Du, Junmin Zhang, Yanping Lei, Hongyun Guo

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

Login to get access

Abstract

Background

Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell line.

Methods

We extracted the CTL by ethanol and assessed the cytotoxicity by CCK-8 method. Cell cycle and apoptosis were detected by a FACS Verse Calibur flow cytometry.

Results

The CTL were extracted by means of ethanol hot refluxing and the content of total lignans in CTL was about 55.77%. By the CCK-8 assays, CTL inhibited the growth of A549 cells in a dose-dependent fashion, with the IC50 values of 39.82 ± 1.74 μg/mL. CTL also inhibited the growth to a less extent in HeLa, HepG2, MKN28 and HT-29 cells.

Conclusion

At low doses, the CTL effectively inhibited the growth of A549 cells. By comparison of IC50 values, we found that A549 cells might be more sensitive to the treatment with CTL. In addition, CTL were also able to increase the population of A549 cells in G2/M phase and the percentage of apoptotic A549 cells. CTL may have therapeutic potential in lung adenocarcinoma cancer by regulating cell cycle and apoptosis.
Literature
1.
Zhang JM, Shi XF, Fan B. Research progress on chemical constituents and pharmacological activities of Cedrus deodara. Chin Trad Pat Med. 2009;31(6):928–33.
2.
Yadav JP, Arya V, Yadav S, Panghal M, Kumar S, Dhankhar S. Cassia occidentalis L.: a review on its ethnobotany, phytochemical and pharmacological profile. Fitoterapia. 2010;81(4):223–30.CrossRef
3.
Patil S, Prakash T, Kotresha D, Rao NR, Pandy N. Antihyperlipidemic potential of Cedrus deodara extracts in monosodium glutamate induced obesity in neonatal rats. Indian J Pharmacol. 2011;43(6):644–7.PubMedPubMedCentral
4.
Sachin BS, Koul M, Zutshi A, Singh SK, Tikoo AK, Tikoo MK, Saxena AK, Sharma SC, Johri RK. Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;862(1–2):237–41.CrossRef
5.
Saab AM, Lampronti I, Borgatti M, Finotti A, Harb F, Safi S, Gambari R. In vitro evaluation of the anti-proliferative activities of the wood essential oils of three Cedrus species against K562 human chronic myelogenous leukaemia cells. Nat Prod Res. 2012;26(23):2227–31.CrossRef
6.
Chaudhary AK, Ahmad S, Mazumder A. Isolation, structural elucidation and in vitro antioxidant activity of compounds from chloroform extract of Cedrus deodara (Roxb.) loud. Nat Prod Res. 2015;29(3):268–73.CrossRef
7.
Wu Y, Bai J, Zhong K, Huang Y, Gao H. A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus. Food Chem. 2017;218:463–70.CrossRef
8.
Bai J, Wu Y, Liu X, Zhong K, Huang Y, Gao H. Antibacterial activity of Shikimic acid from pine needles of Cedrus deodara against Staphylococcus aureus through damage to cell membrane. Int J Mol Sci. 2015;16(11):27145–55.CrossRef
9.
Zhao Z, Dong Z, Ming J, Liu Y. Cedrin identified from Cedrus deodara (Roxb.) G. Don protects PC12 cells against neurotoxicity induced by Abeta1–42. Nat Prod Res. 2017:1–4.
10.
Shi XF, Bai ZH, Liu DY. Li S: [study on the chemical constituent from the dichloromethane extract. Of the pine needles of Cedrus deodara]. Zhong Yao Cai. 2012;35(3):404–6.PubMed
11.
Bai CH, Shi XF, Liu DY. Li S: [study on the chemical constituent from the dichloromethane extract of the pine needle of Cedrus deodara (II)]. Zhong Yao Cai. 2013;36(4):567–9.PubMed
12.
Zhang JM, Shi XF, Li C, Fan B, Wang DD, Liu DY. Study on the chemical constituents from pine needles of Cedrus deodara. Zhong Yao Cai. 2010;33(2):215–8.PubMed
13.
Zhang JM, Shi XF, Ma QH, He FJ, Wang DD, Liu DY. Fan B: [studies on the chemical constituents from pine needles of Cedrus deodara (II)]. Zhong Yao Cai. 2010;33(7):1084–6.PubMed
14.
Liu DY, Shi XF, Wang DD, He FJ, Ma QH, Fan B. Two new myricetin glycosides from pine needles of Cedrus deodara. Chem Nat Compd. 2011;47(5):704–7.CrossRef
15.
Zhang JM, Shi XF, Ma QH, He FJ, Fan B, Wang DD, Liu DY. Chemical constituents from pine needles of Cedrus deodara. Chem Nat Compd. 2011;47(2):272–4.CrossRef
16.
Zhang JM, Shi XF, Fu SW, Zhao J, Guo YL. Separation of acidic compounds and determination of shikimic acid in water extracts of several conifers by HPLC. Chem Nat Compd. 2013;49(4):728–9.CrossRef
17.
Shi X, Liu D, Zhang J, Hu P, Shen W, Fan B, Ma Q, Wang X. Extraction and purification of total flavonoids from pine needles of Cedrus deodara contribute to anti-tumor in vitro. BMC Complement Altern Med. 2016;16:245.CrossRef
18.
Saxena A, Saxena AK, Singh J, Bhushan S. Natural antioxidants synergistically enhance the anticancer potential of AP9-cd, a novel lignan composition from Cedrus deodara in human leukemia HL-60 cells. Chem Biol Interact. 2010;188(3):580–90.CrossRef
19.
Sharma PR, Shanmugavel M, Saxena AK, Qazi GN. Induction of apoptosis by a synergistic lignan composition from Cedrus deodara in human cancer cells. Phytother Res. 2008;22(12):1587–94.CrossRef
20.
Shashi B, Jaswant S, Madhusudana RJ, Kumar SA, Nabi QG. A novel lignan composition from Cedrus deodara induces apoptosis and early nitric oxide generation in human leukemia Molt-4 and HL-60 cells. Nitric Oxide. 2006;14(1):72–88.CrossRef
21.
Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod. 2016;79(3):629–61.CrossRef
22.
Touillaud MS, Thiebaut AC, Fournier A, Niravong M, Boutron-Ruault MC, Clavel-Chapelon F. Dietary lignan intake and postmenopausal breast cancer risk by estrogen and progesterone receptor status. J Natl Cancer Inst. 2007;99(6):475–86.CrossRef
23.
Fini L, Hotchkiss E, Fogliano V, Graziani G, Romano M, De Vol EB, Qin H, Selgrad M, Boland CR, Ricciardiello L. Chemopreventive properties of pinoresinol-rich olive oil involve a selective activation of the ATM-p53 cascade in colon cancer cell lines. Carcinogenesis. 2008;29(1):139–46.CrossRef
24.
Saarinen NM, Warri A, Dings RP, Airio M, Smeds AI, Makela S. Dietary lariciresinol attenuates mammary tumor growth and reduces blood vessel density in human MCF-7 breast cancer xenografts and carcinogen-induced mammary tumors in rats. Int J Cancer. 2008;123(5):1196–204.CrossRef
25.
Yang SE, Hsieh MT, Tsai TH, Hsu SL. Down-modulation of Bcl-XL, release of cytochrome c and sequential activation of caspases during honokiol-induced apoptosis in human squamous lung cancer CH27 cells. Biochem Pharmacol. 2002;63(9):1641–51.CrossRef
26.
Peuhu E, Rivero-Muller A, Stykki H, Torvaldson E, Holmbom T, Eklund P, Unkila M, Sjoholm R, Eriksson JE. Inhibition of Akt signaling by the lignan matairesinol sensitizes prostate cancer cells to TRAIL-induced apoptosis. Oncogene. 2010;29(6):898–908.CrossRef
27.
Rao J M, Srinivas P V, Yadav J S. Herbal chemical composition for the treatment of cancer [P]. US: 6649650B2, 2003-11-18.
28.
Rao J M, Srinivas P V, Yadav J S. Novel herbal composition for the treatment of cancer [P]. US: 2003118676A1, 2003-06-26.
29.
Singh SK, Shanmugavel M, Kampasi H, Singh R, Mondhe DM, Rao JM, Adwankar MK, Saxena AK, Qazi GN. Chemically standardized isolates from Cedrus deodara stem wood having anticancer activity. Planta Med. 2007;73:519–26.CrossRef
30.
Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646–74.CrossRef
31.
Chen T, Stephens PA, Middleton FK, Curtin NJ. Targeting the S and G2 checkpoint to treat cancer. Drug Discov Today. 2012;17:194–202.CrossRef
Metadata
Title
Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells
Authors
Xiaofeng Shi
Ruiqin Du
Junmin Zhang
Yanping Lei
Hongyun Guo
Publication date
01-12-2019
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-019-2682-6

Other articles of this Issue 1/2019

BMC Complementary Medicine and Therapies 1/2019 Go to the issue

Research article

Online yoga in myeloproliferative neoplasm patients: results of a randomized pilot trial to inform future research

Research article

Sheng Mai San protects H9C2 cells against hyperglycemia-induced apoptosis

Research article

The protective effects of polysaccharide extract from Xin-Ji-Er-Kang formula on Ang II-induced HUVECs injury, L-NAME-induced hypertension and cardiovascular remodeling in mice

Research article

Use of traditional and complementary medicine among Norwegian cancer patients in the seventh survey of the Tromsø study

Research article

Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats

Case report

Acute liver failure associated with Fructus Psoraleae: a case report and literature review