Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2019

Open Access 01-12-2019 | Erythromycin | Research article

Antibacterial and antibiotic-modifying activities of fractions and compounds from Albizia adianthifolia against MDR Gram-negative enteric bacteria

Authors: Cedric F. Tchinda, Gaiëlle Sonfack, Ingrid K. Simo, İlhami Çelik, Igor K. Voukeng, Blaise K. Nganou, Gabin T. M. Bitchagno, Sultan Funda Ekti, Mathieu Tene, Pierre Tane, Veronique P. Beng, Victor Kuete

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

Login to get access

Abstract

Background

Albizia adianthifolia (Schum.) is medicinally used in Cameroon to manage bronchitis and skin diseases. Our previous study documented the antibacterial potential of its roots’ methanol extract. In this study, methanol roots extract was subjected to chromatography techniques and fractions (AARa and AARb), sub-fractions (AARa1–4, AARb1–2 and AARb11–14) together with isolated phytochemicals were assessed for their antimicrobial as well as their antibiotic-potentiating effects towards Gram-negative multidrug resistant (MDR) bacteria.

Methods

The antibacterial activities of the samples (determination of Minimal Inhibitory « MIC » and Minimal Bactericidal Concentration « MBC ») were determined by the modified rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay, as well as those of antibiotics in association with the compounds. Column chromatography was applied to isolate phytochemicals from roots extract and their chemical structures were determined using spectroscopic techniques.

Results

The phytochemicals isolated were stearic acid (1), a mixture (1:1) of stigmasterol and β-sitosterol (2 +  3), β-sitosterol 3-O-β-D-glucopyranoside (4), palmatin (5), homomangiferin (6) and mangiferin (7). Fraction AARa exhibited selective inhibitory effects whilst all tested bacteria were inhibited by AARb in MIC ranges of 8 to 1024 μg/mL. Sub-fractions AARb1–2 had MIC values between 8 μg/mL and 1024 μg/mL on all tested bacteria. Phytochemicals 4, 2 +  3 and 7 inhibited the growth of 54.54% (6/11), 45.45% (5/11) and 27.27% (3/11) tested bacterial strains, respectively. When tested with an efflux pumps inhibitor (Phenylalanine-Arginine-β-Naphthylamide or PAβN), the inhibitory effects of compounds 2 + 3 and 4 increased towards all the tested bacteria. In association with erythromycin (ERY), streptomycin (STR) and tetracycline (TET), compounds 2 + 3 and 4 had the most significant synergistic activity on the seven selected bacteria.

Conclusion

The present study provides information on the possible use of Albizia adianthifolia and its constituents in the control of Gram-negative infections including MDR phenotypes.
Appendix
Available only for authorised users
Literature
1.
Adwan G, Mhanna M. Synergistic effects of plant extracts and antibiotics on staphylococcus aureus strains isolated from clinical specimens. J Sci Res. 2008;3:134–9.
2.
Falagas ME, Bliziotis IA. Pandrug-resistant gram-negative bacteria the dawn of the post antibiotic era. Inter J Antimicro Agts. 2007;29:630–6.CrossRef
3.
Amgad AA, Martin RPJ, Ismail MM, Abdelkareem MA, Ahmad MA, Mohamed EH. Antimicrobial activities of seed extracts of mango (MangiferaindicaL.). Adv Microbiol. 2012;2:571–6.CrossRef
4.
Cattoir V. Pompes d’efflux et résistance aux antibiotiques chez les bactéries. Path Biol. 2004;52:607–16.CrossRef
5.
O. Lomovskaya O, Bostian KA. Practical applications and feasibility of efflux pump inhibitors in the clinic–a vision for applied use. Biochem Pharmacol 2006; 71:910–918.PubMedCrossRef
6.
Dzotam KJ, Simo KI, Bitchagno G, Ilhami C, Sandjo PL, Tane P, Kuete V. In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3′, 4′, 7- thihydroxyflavone from Myristica fragans Houtt against MDR gram-negative enteric bacteria. BMC Complement Altern Med. 2018;18:15.PubMedPubMedCentralCrossRef
7.
Kadhim MI, Rana KN, Amaal SA. Antibacterial activity of nutmeg (Myristica fragrans) seed extracts against some pathogenic bacteria. J Al-Nahrain Univ. 2013;16(2):188–92.CrossRef
8.
Djeussi SD, Sandjo PL, Noumedem AJ, Omosa KL, Kuete V. Antibacterial activities of the methanol extracts and compounds from Erythrina sigmoidea against gram-negative multi-drug resistant phenotypes. BMC Complement Altern Med. 2015.
9.
Voukeng IK, Kuete V, Dzoyem PJ, Fankam GA, Noumedem AJ, Kuiate RJ, Pages MJ. Antibacterial and antibiotic-potentiation activities of the methanol extract of some Cameroonian spices against gram-negative multidrug resistant phenotypes. BMC Complement Altern Med. 2012;5:299.
10.
Voukeng IK, Beng VP, Kuete V. Antibacterial activity of six medicinal Cameroonian plants against gram-positive and gram-negative multidrug resistant phenotypes. BMC Complement Altern Med. 2016.
11.
Fankam GA, Kuiate RG, Kuete V. Antibacterial and antibiotic resistance modifying activity of the extracts from Allanblackia gabonensis, Combretum mole and Gladiolus quartinianus against gram-negative bacteria including multidrug resistant phenotypes. BMC Complement Altern Med. 2015.
12.
Tankeo BS, Damen F, Sandjo PL, Ilhami C, Tane P, Kuete V. Antibacterial activities of the methanol extracts, fractions and compounds from Harungana madagascariensis lam. Ex pair (Hypericaceae). J Ethnopharmacol. 2016;90:100–5.CrossRef
13.
Van-Wyk B, Gerick N. People’s plants: a guide to useful plants of southern Africa. Pretoria: Briza publications; 2000.
14.
Watt J, Breyer-Brandwyk M. The medicinal and poisonous plants of southern and Easthern Africa. 2nd ed. London: Livingstone; 1962.
15.
Haddad M, Laurens V, Lacaille -Dubois AM. Induction of apoptosis in a leukemia cell line by triterpene saponins from Albizia adianthifolia. Bioorg Med Chem. 2004;12:4725–34.PubMedCrossRef
16.
Haddad M, Miyamoto T, Laurens V, Lacaille-Dubois AM. Two new biologically active triterpenoidal saponins acylated with salicylic acid from Albizia adianthifolia. J Nat Prods. 2003;66:372–7.CrossRef
17.
Tamokou DJ, Simo JDM, Keilah PL, Tene M, Tane P, Kuiate RJ. Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae). BMC Complement Altern Med. 2012.
18.
Haddad M, Khan AI, Lacaille-Dubois AM. Two new prosapogenins from Albizia adianthifolia. Pharmazie. 57:705–8.
19.
20.
Kuete V, Wabo FG, Ngameni B, Mbaveng TA, Metuno R, Etoa F-X, Lall N. Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvingia gabonensis (Ixonanthaceae). J Ethnopharmacol. 2007;114(1):54–60.PubMedCrossRef
21.
Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;64:711–3.PubMedCrossRef
22.
Kuete V, Nana F, Ngameni B, T. A. Mbaveng AT, Keumedjio F, Ngadjui BT. Antimicrobial activity of the crude extract, fractions and compounds from stem bark of Ficus ovate (Moraceae). J Ethnopharmacol 2009; 124:556–56.PubMedCrossRef
23.
Noumedem J, Mihasan M, Kuiate J, Stefan M, Cojocaru D, Dzoyem J, Kuete V. In vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species. BMC Complement Altern Med. 2013;13:190.PubMedPubMedCentralCrossRef
24.
25.
Coutinho HD, Vasconcellos A, Freire-Pessoa HL, Gadelha CA, Gadelha TS, Almeida-Filho GG. Natural products from the termite Nasutitermes corniger lower aminoglycoside minimum inhibitory concentrations. Pharmacogn Mag. 2010;6:1–4.PubMedPubMedCentralCrossRef
26.
Braga LC, Leite AA, KGS X, Takahashi JA, Bemquerer MP, Chartone-Souza E, Nascimento AM. Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can J Microbiol. 2005;51(7):541–7.PubMedCrossRef
27.
Weast RC, Astle JM. CRC handbook of data on organic compounds. Volumes I and II, Boca Raton, FL: CRC Press Inc. s. 1985. 1–927.
28.
De-Ekmamkul W, Potduang B. B. Biosynthesis of beta-sitosterol and stigmasterol in Croton sublyratus proceeds via a mixed origin of isoprene units. Phytochemistry. 2003;62(3):389–93.CrossRef
29.
Ramaiarantsoa H, Koffi AB, Assi AM, Djakoure LA. Les 3-O-β-D-glucoside du β-sitostérol isolés des feuilles de Ravenala madagascariensis. Journal de la Société Ouest-africaine de chine. 2008;26:99–103.
30.
Ling-Ling Y, Rong-Tao L, Yuan-Bao A, Wei L, Zhang-Shuang D, Zhong-Mei Z. Protoberberine Isoquinoline alkaloids from Arcangelisia gusanlung. Molecules. 2014;19:13332–41.CrossRef
31.
Dineshkumar B, Analava M, Manjunatha M. Studies on the anti-diabetic and hypolipidemic potentials of mangiferin (Xanthone Glucide) in streptozotocin-induced type 1 and type 2 diabetic model rats. Inter J Adv Pharma Scien. 2010;1:75–85.CrossRef
32.
De Souza RRJ. Mangiferin: Microencapsulation in Pectin/Chitosan Systems, in vitro intestinal metabolism and anticancer activity. Ph.D in Chemistry, University of Cearà, 2012. 1–242.
33.
34.
Sharma SK, Singh AP. Antimicrobial investigations on rhizomes of Cyperus rotundus Linn. Pharm Lett. 2011;3(3):427–31.
35.
Kuete V, Ngameni B, Tangmouo GJ, Bolla JM, Alibert-Franco S, Ngadjui TB, Pagès JM. Efflux pumps are involved in the defense of gram-negative Bacteria against the natural products Isobavachalcone and Diospyrone. Antimicrob Agents Chemother. 2010;54(5):1749–52.PubMedPubMedCentralCrossRef
36.
Mims C, Playfair J, Roitt I, Wakelin D, Williams R. Antimicrobials and chemotherapy. In: Mims, C.A. (Eds.). Med Microbiol Rev 1993; 35:1–34.
37.
Mbaveng AT, Kuete V, Mapunya BM, Beng VP, Nkengfack AE, Meyer JJ, Lall N. Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities. Envir Toxicol and Pharmacol. 2011;32(2):162–7.
38.
Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med. 2010;76:1–13.CrossRef
39.
Chevalier J, Pagès JM, Eyraud A, Malléa M. Membrane permeability modifications are involved in antibiotic resistance in Klebsiella pneumoniae. Biochem Biophys Res Commun. 2000;274:496–9.PubMedCrossRef
40.
Ghisalberti D, Masi M, Pagès JM, Chevalier J. Chloramphenicol and expression of multidrug efflux pump in Enterobacter aerogenes. Biochem Biophys Res Commun. 2005;328:1113–8.PubMedCrossRef
41.
Pagès JM, Amiral L. Mechanisms of drug efflux and strategies to combat them: challenging the efflux pump of gram-negative bacteria. Biochem and Biophys Act. 2009;1794:826–33.
42.
Lamers RP, Cavallari JF, Burrows LL. The efflux inhibitor phenylalanine arginine beta-naphtylamide permeabilizes the outer membrane of gram-negative bacteria. PLoS One. 2013;8:e60666.PubMedPubMedCentralCrossRef
Metadata
Title
Antibacterial and antibiotic-modifying activities of fractions and compounds from Albizia adianthifolia against MDR Gram-negative enteric bacteria
Authors
Cedric F. Tchinda
Gaiëlle Sonfack
Ingrid K. Simo
İlhami Çelik
Igor K. Voukeng
Blaise K. Nganou
Gabin T. M. Bitchagno
Sultan Funda Ekti
Mathieu Tene
Pierre Tane
Veronique P. Beng
Victor Kuete
Publication date
01-12-2019
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-019-2537-1

Other articles of this Issue 1/2019

BMC Complementary Medicine and Therapies 1/2019 Go to the issue

Research article

Application evaluation of clinical practice guidelines for traditional Chinese medicine: a clinical analysis based on the analytic hierarchy process

Research article

Treatment satisfaction in Chinese medicine outpatient care: a comparison of patients’ and doctors’ views

Research article

Decreased fracture incidence with traditional Chinese medicine therapy in patients with osteoporosis: a nationwide population-based cohort study

Research article

Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines

Research article

The synergism of Clinacanthus nutans Lindau extracts with gemcitabine: downregulation of anti-apoptotic markers in squamous pancreatic ductal adenocarcinoma

Research article

Helminthostachys zeylanica alleviates hepatic steatosis and insulin resistance in diet-induced obese mice