Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Identification of antitumoral agents against human pancreatic cancer cells from Asteraceae and Lamiaceae plant extracts

Authors: Lamia Mouhid, Marta Gómez de Cedrón, Teodoro Vargas, Elena García-Carrascosa, Nieves Herranz, Mónica García-Risco, Guillermo Reglero, Tiziana Fornari, Ana Ramírez de Molina

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

Login to get access

Abstract

Background

Pancreatic cancer is one of the most aggressive and mortal cancers. Although several drugs have been proposed for its treatment, it remains resistant and new alternatives are needed. In this context, plants and their derivatives constitute a relevant source of bioactive components which might efficiently inhibit tumor cell progression.

Methods

In this study, we have analyzed the potential anti-carcinogenic effect of different Asteraceae (Achillea millefolium and Calendula officinalis) and Lamiaceae (Melissa officinalis and Origanum majorana) plant extracts obtained by different green technologies (Supercritical CO2 Extraction –SFE- and Ultrasonic Assisted Extraction –UAE-) to identify efficient plant extracts against human pancreatic cancer cells that could constitute the basis of novel treatment approaches.

Results

Asteraceae extracts showed better results as antitumoral agents than Lamiaceae by inducing cytotoxicity and inhibiting cell transformation, and SFE extracts were most efficient than UAE extracts. In addition, SFE derived plant extracts from Achillea millefolium and Calendula officinalis displayed synergism with the chemotherapeutic 5-Fluororacil.

Conclusion

These results show how Yarrow and Marigold SFE-derived extracts can inhibit pancreatic cancer cell growth, and could be proposed for a comprehensive study to determine the molecular mechanisms involved in their bioactivity with the final aim to propose them as potential adjuvants in pancreatic cancer therapy.
Appendix
Available only for authorised users
Literature
1.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69–90.CrossRef
2.
Ducreux M, Caramella C, Hollebecque A, Burtin P, Goéré D, Seufferlein T, Haustermans K, Van Laethem J, Conroy T, Arnold D. Cancer of the pancreas: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2015;26(suppl_5):v56–68.CrossRef
3.
Mouhid L, Corzo-Martínez M, Torres C, Vázquez L, Reglero G, Fornari T. Ramírez de Molina a: improving in vivo efficacy of bioactive molecules: an overview of potentially antitumor phytochemicals and currently available lipid-based delivery systems. Journal of Oncology. 2017;2017.
4.
Gonzalez-Vallinas M, Gonzalez-Castejon M, Rodriguez-Casado A. Ramirez de Molina a: dietary phytochemicals in cancer prevention and therapy: a complementary approach with promising perspectives. Nutr Rev. 2013;71(9):585–99.CrossRef
5.
García-Risco MR, Mouhid L, Salas-Pérez L, López-Padilla A, Santoyo S, Jaime L, de Molina AR, Reglero G, Fornari T: Biological activities of Asteraceae (Achillea millefolium and Calendula officinalis) and Lamiaceae (Melissa officinalis and Origanum majorana) plant extracts. Plant Foods Hum Nutr 2017, 72(1):96–102.CrossRef
6.
Savini I, Arnone R, Catani MV and Avigliano L. Origanum vulgare induces apoptosis in human colon cancer caco2 cells. Nutr Cancer. 2009;61(3):381–9.CrossRef
7.
Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, Telci İ. Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. J Sci Food Agric. 2015.
8.
Weidner C, Rousseau M, Plauth A, Wowro S, Fischer C, Abdel-Aziz H, Sauer S. Melissa officinalis extract induces apoptosis and inhibits proliferation in colon cancer cells through formation of reactive oxygen species. Phytomedicine. 2014.
9.
Saraydin SU, Tuncer E, Tepe B, Karadayi S, Ozer H, Sen M, Karadayi K, Inan D, Elagoz S, Polat Z. Antitumoral effects of Melissa officinalis on breast cancer in vitro and in vivo. Asian Pac J Cancer Prev. 2012;13(6):2765–70.CrossRef
10.
Csupor-Löffler B, Hajdú Z, Zupkó I, Réthy B, Falkay G, Forgo P, Hohmann J. Antiproliferative effect of flavonoids and sesquiterpenoids from Achillea millefolium sl on cultured human tumour cell lines. Phytother Res. 2009;23(5):672–6.CrossRef
11.
Ghavami G, Sardari S, Shokrgozar MA. Anticancerous potentials of Achillea species against selected cell lines. J Medicinal Plants Research. 2010;4(22):2411–7.
12.
Ukiya M, Akihisa T, Yasukawa K, Tokuda H, Suzuki T, Kimura Y. Anti-inflammatory, anti-tumor-promoting, and cytotoxic activities of constituents of marigold (Calendula officinalis) flowers. J Nat Prod. 2006;69(12):1692–6.CrossRef
13.
Jimenez-Medina E, Garcia-Lora A, Paco L, Algarra I, Collado A, Garrido F. A new extract of the plant Calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation. BMC Cancer. 2006;6:119.CrossRef
14.
Monks A, Scudiero D, Skehan P, Shoemaker R, Paull K, Vistica D, Hose C, Langley J, Cronise P, Vaigro-Wolff A. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst. 1991;83(11):757–66.CrossRef
15.
Chou T, Talalay P. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzym Regul. 1984;22:27–55.CrossRef
16.
Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, Minor L. Cell Viability Assays. In: Sittampalam GS, Coussens NP, Brimacombe K, et al., editors. Assay Guidance Manual: Bethesda (MD); 2004.
17.
Fryer RA, Barlett B, Galustian C, Dalgleish AG. Mechanisms underlying gemcitabine resistance in pancreatic cancer and sensitisation by the iMiD lenalidomide. Anticancer Res. 2011;31(11):3747–56.PubMed
18.
Huanwen W, Zhiyong L, Xiaohua S, Xinyu R, Kai W, Tonghua L. Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines. Mol Cancer. 2009;8(125):21.
19.
Preethi KC, Siveen KS, Kuttan R, Kuttan G. Inhibition of metastasis of B16F-10 melanoma cells in C57BL/6 mice by an extract of Calendula officinalis L flowers. Asian Pac J Cancer Prev. 2010;11(6):1773–9.PubMed
20.
Dias MI, Barros L, Dueñas M, Pereira E, Carvalho AM, Alves RC, Oliveira MBP, Santos-Buelga C, Ferreira IC. Chemical composition of wild and commercial Achillea millefolium L. and bioactivity of the methanolic extract, infusion and decoction. Food Chem. 2013;141(4):4152–60.CrossRef
21.
Abdel-Massih RM, Fares R, Bazzi S, El-Chami N, Baydoun E. The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line. Leuk Res. 2010;34(8):1052–6.CrossRef
22.
Lin J, Chen Y, Lee Y, Hou CR, Chen F, Yang D. Antioxidant, anti-proliferative and cyclooxygenase-2 inhibitory activities of ethanolic extracts from lemon balm (Melissa officinalis L.) leaves. LWT-Food Science and Technology. 2012;49(1):1–7.CrossRef
23.
González-Vallinas M, Molina S, Vicente G, de la Cueva A, Vargas T, Santoyo S, García-Risco MR, Fornari T, Reglero G, de Molina AR. Antitumor effect of 5-fluorouracil is enhanced by rosemary extract in both drug sensitive and resistant colon cancer cells. Pharmacol Res. 2013;72:61–8.CrossRef
24.
Kienle GS, Kiene H. Review article: influence of Viscum album L (European mistletoe) extracts on quality of life in cancer patients: a systematic review of controlled clinical studies. Integrative cancer therapies. 2010;9(2):142–57.CrossRef
25.
Tröger W, Galun D, Reif M, Schumann A, Stanković N, Milićević M. Viscum album [L.] extract therapy in patients with locally advanced or metastatic pancreatic cancer: a randomised clinical trial on overall survival. Eur J Cancer. 2013;49(18):3788–97.CrossRef
Metadata
Title
Identification of antitumoral agents against human pancreatic cancer cells from Asteraceae and Lamiaceae plant extracts
Authors
Lamia Mouhid
Marta Gómez de Cedrón
Teodoro Vargas
Elena García-Carrascosa
Nieves Herranz
Mónica García-Risco
Guillermo Reglero
Tiziana Fornari
Ana Ramírez de Molina
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2322-6

Other articles of this Issue 1/2018

BMC Complementary Medicine and Therapies 1/2018 Go to the issue

Research article

Anti-Brucella activity of Caryopteris mongolica Bunge root extract against Brucella melitensis infection in mice

Research article

Herbal medicine use by pregnant women in Bangladesh: a cross-sectional study

Study protocol

Impact of a structured yoga program on blood pressure reduction among hypertensive patients: study protocol for a pragmatic randomized multicenter trial in primary health care settings in Nepal

Research article

Herbal formula Xinshuitong capsule exerts its cardioprotective effects via mitochondria in the hypoxia-reoxygenated human cardiomyocytes

Research article

Phenolic profile and biological properties of the leaves of Ficus vasta Forssk. (Moraceae) growing in Egypt

Research article

In-vitro osteoblast proliferation and in-vivo anti-osteoporotic activity of Bombax ceiba with quantification of Lupeol, gallic acid and β-sitosterol by HPTLC and HPLC