Top

BMC Complementary Medicine and Therapies

Published in:

Open Access 01-12-2018 | Research article

Sources for developing new medicinal products: biochemical investigations on alcoholic extracts obtained from aerial parts of some Romanian Amaryllidaceae species

Authors: Daniela Benedec, Ilioara Oniga, Daniela Hanganu, Ana Maria Gheldiu, Cristina Pușcaș, Radu Silaghi-Dumitrescu, Mihaela Duma, Brîndușa Tiperciuc, Rodica Vârban, Laurian Vlase

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

Abstract

Background

Although Galanthus nivalis L. (snowdrop) is known for the galanthamine content, used in the treatment of Alzheimer disease, the polyphenolic compounds of Amaryllidaceae species are less studied. Proper understanding of the polyphenolics in these extracts and of their antioxidant and antimicrobial properties may allow a reconsideration of their medicinal uses.

Methods

The polyphenolic content of four selected Amaryllidaceae species harvested from Romania (Galanthus nivalis L., Narcissus pseudonarcissus L., N. poeticus L. and Leucojum vernum L.) was determined by spectrophotometric methods; the identification of phenolic compounds was performed by a HPLC-MS method, in order to establish their polyphenolic fingerprints. For the evaluation of the antioxidant potential the following methods were employed: DPPH radical scavenging, FRAP, hemoglobin ascorbate peroxidase activity inhibition (HAPX), inhibition of lipid peroxidation catalyzed by cytochrome c, and electron paramagnetic resonance (EPR) spectroscopy assays. Antimicrobial activity was assessed using the disc diffusion method.

Results

Qualitative and quantitative analyses highlight important amount of polyphenols (over 15 mg/g); the main identified compounds are chlorogenic and p-coumaric acids in all species. Only G. nivalis shows antioxidant activity by all the used methods. G. nivalis and L. vernum strongly inhibits the growth of S. aureus, while N. poeticus shows a very good antifungal activity.

Conclusions

The results of this study provide a new approach to the properties and therapeutic uses of some Romanian widespread Amaryllidaceae species that could be considered sources of developing new medicinal products with anti anti-staphylococcal and antifungal activity.

Sârbu I, Ştefan N, Oprea A. Plante vasculare din România. Determinator ilustrat de teren. Bucharest: (Vascular plants of Romania) Victor B Victor; 2013. p. 991–3.

Berkov S, Codina C, Bastid J. The genus Galanthus: a source of bioactive compounds. In: Rao V, editor. Phytochemicals - a global perspective of their role in nutrition and health. InTech; 2012. p. 235–54.

Takos AM, Rook F. Towards a molecular understanding of the biosynthesis of Amaryllidaceae alkaloids in support of their expanding medical use. Int J Mol Sci. 2013; https://doi.org/10.3390/ijms140611713.

Bokov DO, Samylina IA. Identification of flavonoids in homeopathic mother tinctures of Galanthus woronowii Losinsk. and Galanthus nivalis L. by ultra-performance liquid chromatography with photodiode arrays and tandem quadrupole mass-selective detectors. Pharm Chem J. 2016; https://doi.org/10.1007/s11094-016-1469-z.

Bokov DO, Malinkin AD, Samylina IA, Vladimirovich BV. Hydroxycinnamic and organic acids of snowdrops (Galanthus L.). J Appl Pharm Sci. 2017; https://doi.org/10.7324/JAPS.2017.70205. https://www.researchgate.net/publication/315066255_Hydroxycinnamic_and_organic_acids_of_snowdrops_Galanthus_L.

Ločárek M, Nováková J, Klouček P, Hošt'álkoviá A, Kokoška L, Gábrlová L, Šafratová M, Opletal L, Cahliková L. Antifungal and antibacterial activity of extracts and alkaloids of selected Amaryllidaceae species. Nat Prod Commun. 2015; https://www.researchgate.net/publication/290439314_Antifungal_and_Antibacterial_Activity_of_Extracts_and_Alkaloids_of_Selected_Amaryllidaceae_Species.

Orhan I, Sener B. Bioactivity-directed fractionation of alkaloids from some Amaryllidaceae plants and their anticholinesterase activity. Chem Nat Compd. 2003; https://www.researchgate.net/publication/227196428_Bioactivity-Directed_Fractionation_of_Alkaloids_from_Some_Amaryllidaceae_Plants_and_Their_Anticholinesterase_Activity.

Elgorashi EE, Zschocke S, Van Staden J. The anti-inflammatory and antibacterial activities of Amaryllidaceae alkaloids. S Afr J Bot. 2003; https://doi.org/10.1016/S0254-6299(15)30329-X.

Conforti F, Loizzo MR, Marrelli M, Menichini F, Statti GA, Uzunov D, Menichini F. Quantitative determination of Amaryllidaceae alkaloids from Galanthus reginae-olgae subsp. vernalis and in vitro activities relevant for neurodegenerative diseases. Pharm Biol. 2010; https://doi.org/10.3109/13880200903029308.

10.

Resetár A, Freytag C, Kalydi F, Gonda S, M-Hamvas M, Ajtay K, Papp L, Máthé C. Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species. Acta Soc Bot Pol. 2017; https://doi.org/10.5586/asbp.3525.

11.

Tuzen M, Ozdemir M. Chromatographic determination of phenolic acids in the snowdrop by HPLC. Turk J Chem. 2003; https://www.researchgate.net/publication/279694582_Chromatographic_determination_of_phenolic_acids_in_the_snowdrop_by_HPLC.

12.

Ivancheva S, Nikolova M, Tsvetkova R. Pharmacological activities and biologically active compounds of Bulgarian medicinal plants. In: Phytochemistry: Advances in researc, Imperato F ed. Kerala: Research Signpost; 2006. p. 87–103.

13.

Li X, Lu M, Tang D, Shi Y. Composition of carotenoids and flavonoids in Narcissus cultivars and their relationship with flower color. PLoS One. 2015; https://doi.org/10.1371/journal.pone.0142074. eCollection 2015.

14.

Nikolova M, Gevrenova R. Determination of phenolic acids in Amaryllidaceae species by high performance liquid chromatography. Pharm Biol. 2005; https://doi.org/10.1080/13880200590928906

15.

Vlase L, Benedec D, Hanganu D, Damian G, Csillag I, Sevastre B, Mot AC, Silaghi-Dumitrescu R, Tilea I. Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Molecules. 2014; https://doi.org/10.3390/molecules19055490.

16.

Benedec D, Hanganu D, Oniga I, Tiperciuc B, Olah N, Raita O, Bischin C, Silaghi R, Vlase L. Assessment of rosmarinic acid content in six Lamiaceae species extracts and their antioxidant and antimicrobial potential. Pak J Pharm Sci. 2015;28(6):2297–303.PubMed

17.

Benedec D, Oniga I, Muresan B, Mot AC, Damian G, Nistor A, Silaghi-Dumitrescu R, Hanganu D, Duma M, Vlase L. Contrast between water and ethanol based antioxidant assays: aspen (Populus tremula) and black poplar (Populus nigra) extracts as a case study. J Food Qual. 2014; https://doi.org/10.1111/jfq.12090.

18.

Benedec D, Hanganu D, Oniga I, Filip L, Bischin C, Silaghi-Dumitrescu R, Tiperciuc B, Vlase L. Achillea schurii flowers: chemical, antioxidant, and antimicrobial investigations. Molecules. 2016; https://doi.org/10.3390/molecules21081050.

19.

Anton AM, Pintea AM, Rugina DO, Sconta ZM, Hanganu D, Vlase L, Benedec D. Preliminary studies on the chemical characterization and antioxidant capacity of polyphenols from Sambucus sp. Dig J Nanomater Bios. 2013;3:973–80.

20.

Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005; https://doi.org/10.1021/jf0502698.

21.

Tamokou JDD, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, Nkeng Efouet PA, Wabo HK, et al. Anticancer and antimicrobial activities of some antioxidant-rich Cameroonian medicinal plants. PLoS One. 2013; https://doi.org/10.1371/journal.pone.0055880.

22.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152–78.CrossRef

23.

Council of Europe, editor. European Pharmacopoeia. 5th ed. Council of Europe. Strasbourg: Cedex; 2005. p. 221.

24.

Romanian Pharmacopoeia Commission National Medicines Agency. Romanian Pharmacopoeia. Xth ed. Bucharest: Medical Publishing House; 1993. p. 335.

25.

Nastasa C, Tiperciuc B, Duma M, Benedec D, Oniga O. New hydrazones bearing thiazole scaffold: synthesis, characterization, antimicrobial, and antioxidant investigation. Molecules. 2015; https://doi.org/10.3390/molecules200917325.

26.

Liu H, Qiu N, Ding H, Yao R. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Res Int. 2008; https://doi.org/10.1016/j.foodres.2007.12.012.

27.

Pisoschi AM, Negulescu GP. Methods for total antioxidant activity determination: a review. Biochem Anal Biochem. 2011; https://doi.org/10.4172/2161-1009.1000106.

28.

Cooper CE, Silaghi-Dumitrescu R, Rukengwa M, Alayash AI, Buehler PW. Peroxidase activity of hemoglobin towards ascorbate and urate: a synergistic protective strategy against toxicity of hemoglobin-based oxygen carriers (HBOC). Biochim Biophys Acta. 2008; https://doi.org/10.1016/j.bbapap.2008.03.019.

29.

Mot AC, Damian G, Sarbu C, Silaghi-Dumitrescu R. Redox reactivity in propolis: direct detection of free radicals in basic medium and interaction with hemoglobin. Redox Rep. 2009; https://doi.org/10.1179/135100009X12525712409814.

30.

Mot AC, Bischin C, Damian G, Silaghi-Dumitrescu R. Antioxidant activity evaluation involving hemoglobin-related free radical reactivity. Methods Mol Biol. 2015; https://doi.org/10.1007/978-1-4939-1441-8_18.

31.

Mot AC, Bischin C, Muresan B, Parvu M, Damian G, Vlase L, Silaghi DR. Antioxidant activity evaluation by physiologically relevant assays based on hemoglobin peroxidase activity and cytochrome c-induced oxidation of liposomes. Nat Prod Res. 2015; https://doi.org/10.1080/14786419.2015.1054824.

32.

Benedec D, Hanganu D, Filip L, Oniga I, Tiperciuc B, Olah NK, Gheldiu AM, Raita O, Vlase L. Chemical, antioxidant and antibacterial studies of Romanian Heracleum sphondylium. Farmacia (Bucharest, Rom). 2017;65:252–6.

33.

Pașca C, Mărghitaș L, Dezmirean D, Bobiș O, Bonta V, Chirilă F, Matei I, Fiț N. Medicinal plants based products tested on pathogens isolated from mastitis milk. Molecules. 2017; https://doi.org/10.3390/molecules22091473.

34.

Masike K, Msizi I, Mhlongo MI, Mudau SP, Nobela O, Ncube EN, Tugizimana F, George MS, Madala NE. Highlighting mass spectrometric fragmentation differences and similarities between hydroxycinnamoyl-quinic acids and hydroxycinnamoyl- isocitric acids. Chem Cent J. 2017; https://doi.org/10.1186/s13065-017-0262-8.

35.

Ibrahim RM, El-Halawany AM, Saleh DO, Naggar MB, El-Shabrawy, Bel-Hawary S. HPLC-DAD-MS/MS profiling of phenolics from Securigera securidaca flowers and its anti-hyperglycemic and anti-hyperlipidemic activities. Rev Bras Farmacogn. 2015;25:134–41.CrossRef

36.

Maalik A, Bukhari SM, Zaidi A, Shah KH, Khan FA. Chlorogenic acid: a pharmacologically potent molecule. Acta Pol Pharm. 2016;73:851–4.PubMed

37.

Oboh G, Agunloye OM, Akinyemi AJ, Ademiluyi AO, Adefegha SA. Comparative study on the inhibitory effect of caffeic and chlorogenic acids on key enzymes linked to Alzheimer's disease and some pro-oxidant induced oxidative stress in rats' brain-in vitro. Neurochem Res. 2013; https://doi.org/10.1007/s11064-012-0935-6.

38.

Kim SJ, Um JY, Lee JY. Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-κB activation in mouse peritoneal macrophages. Am J Chin Med. 2011; https://doi.org/10.1142/S0192415X11008737.

39.

Amensour M, Sendra E, Abrini J, Pérez-Alvarez JA, Fernández-López J. Antioxidant activity and total phenolic compounds of myrtle extracts. CyTA--J Food. 2010; https://doi.org/10.1080/19476330903161335.

40.

Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem. 2007;104:1372–8.CrossRef

41.

Alali FQ, Tawaha K, El-Elimat T, Syouf M, El-Fayad M, Abulaila K, Nielsen SJ, Wheaton WD 3rd, Falkinham JO, Oberlies NH. Antioxidant activity and total phenolic content of aqueous and methanolic extracts of Jordanian plants: an ICBG project. Nat Prod Res. 2007; https://doi.org/10.1080/14786410701590285.

42.

Stanciu G, Chirila E, Dobrinas S, Negreanu-Pirjo T. Studies regarding the determination of antioxidant properties of new plant extracts for cosmetic purposes. Rev Chim (Bucharest, Rom). 2010;61:41–4.

43.

Wang L, Bi C, Cai H, Liu B, Zhong X, Deng X, Wang T, Xiang H, Niu X, Wang D. The therapeutic effect of chlorogenic acid against Staphylococcus aureus infection through sortase A inhibition. Front Microbiol. 2015; https://doi.org/10.3389/fmicb.2015.01031.

44.

Li G, Wang X, Xu Y, Zhang B, Xia X. Antimicrobial effect and mode of action of chlorogenic acid on Staphylococcus aureus. Eur Food Res Tech. 2014; https://link.springer.com/article/10.1007/s00217-013-2140-5.

Title: Sources for developing new medicinal products: biochemical investigations on alcoholic extracts obtained from aerial parts of some Romanian Amaryllidaceae species
Authors: Daniela Benedec
Ilioara Oniga
Daniela Hanganu
Ana Maria Gheldiu
Cristina Pușcaș
Radu Silaghi-Dumitrescu
Mihaela Duma
Brîndușa Tiperciuc
Rodica Vârban
Laurian Vlase
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-018-2292-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Sources for developing new medicinal products: biochemical investigations on alcoholic extracts obtained from aerial parts of some Romanian Amaryllidaceae species

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2018

Gelidiella acerosa inhibits lung cancer proliferation

Gender specific association between the use of complementary and alternative medicine (CAM) and alcohol consumption and injuries caused by drinking in the sixth Tromsø study

Prevalence and correlates of herbal medicine use among type 2 diabetic patients in Teaching Hospital in Ethiopia: a cross-sectional study

Anti-methicillin-resistance Staphylococcus aureus (MRSA) compounds from Bauhinia kockiana Korth. And their mechanism of antibacterial activity

Beneficial effect of compound essential oil inhalation on central fatigue

Traditional healing practices in rural Bangladesh: a qualitative investigation