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BMC Complementary Medicine and Therapies

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Open Access 01-12-2018 | Research article

The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells

Authors: Rabiatul Adawiyah Razali, Nik Ahmad Hafiz Nik Ahmad Eid, Turkambigai Jayaraman, Muhammad Asyrafi Amir Hassan, Nabilah Qistina Azlan, Nur Farhana Ismail, Nur Qisya Afifah Veronica Sainik, Muhammad Dain Yazid, Yogeswaran Lokanathan, Aminuddin Bin Saim, Ruszymah Bt Hj Idrus

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

Abstract

Background

One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs).

Methods

Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05% OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05% OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3.

Results

There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05).

Conclusion

The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation.

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Title: The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
Authors: Rabiatul Adawiyah Razali
Nik Ahmad Hafiz Nik Ahmad Eid
Turkambigai Jayaraman
Muhammad Asyrafi Amir Hassan
Nabilah Qistina Azlan
Nur Farhana Ismail
Nur Qisya Afifah Veronica Sainik
Muhammad Dain Yazid
Yogeswaran Lokanathan
Aminuddin Bin Saim
Ruszymah Bt Hj Idrus
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-018-2250-5

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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