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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Neuro-protective effect of rutin against Cisplatin-induced neurotoxic rat model

Authors: Mashal M. Almutairi, Wael A. Alanazi, Musaad A. Alshammari, Moureq Rashed Alotaibi, Ali R. Alhoshani, Salim Salah Al-Rejaie, Mohamed M. Hafez, Othman A. Al-Shabanah

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Cisplatin is widely used chemotherapeutic agent for cancer treatment with limited uses due to its neurotoxic side effect. The aim of this study was to determine the potential preventive effects of rutin on the brain of cisplatin- neurotoxic rat model.

Methods

Forty rats were divided into four groups. Group-1 (control group) was intra-peritoneal (IP) injected with 2.5 ml/kg saline. Group-2 (rutin group) was orally administrated 30 mg/kg rutin dissolved in water for 14 days. Group-3 (cisplatin group) was IP received 5 mg/kg cisplatin single dose. Group-4 (rutin and cisplatin group) was orally administrated 30 mg/kg rutin dissolved in water for 14 days with a single dose of 5 mg/kg cisplatin IP on day ten. Brain tissues from frontal cortex was used to extract RNA, the gene expression levels of paraoxonase-1 (PON-1), PON-2, PON-3, peroxisome proliferator-activated receptor delta (PPAR-δ), and glutathione peroxidase (GPx) was investigated by Real-time PCR.

Results

Cisplatin significantly decreased the expression levels of PON-1, PON-3, PPAR-δ and GPX whereas significantly increased PON-2 expression levels. Co-administration of Rutin prevented the cisplatin-induced toxicity by restoring the alteration in the studied genes to normal values as in the control group.

Conclusion

This study showed that Rutin has neuroprotective effect and reduces cisplatin- neurotoxicity with possible mechanism via the antioxidant pathway.
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Metadata
Title
Neuro-protective effect of rutin against Cisplatin-induced neurotoxic rat model
Authors
Mashal M. Almutairi
Wael A. Alanazi
Musaad A. Alshammari
Moureq Rashed Alotaibi
Ali R. Alhoshani
Salim Salah Al-Rejaie
Mohamed M. Hafez
Othman A. Al-Shabanah
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1976-9

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