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Neuroprotective Effects of Rutin in Streptozotocin-Induced Diabetic Rat Retina

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Abstract

Diabetic retinopathy is widely recognized as a neurodegenerative disease of the eye. Increased oxidative stress has been considered the central factor in damaging neural retina in diabetes. Flavonoids, being powerful antioxidants, play protective roles in several oxidative stress-mediated neurodegenerative diseases. In this study, we analyzed the neuroprotective effects of a potential flavonoid, rutin, in the diabetic rat retina. Diabetes was induced in male Wistar rats by single injection of streptozotocin (65 mg/kg). In age-matched control (non-diabetic) and 1 week of diabetic rats, rutin (100 mg/kg/day) was orally administered and continued for 5 weeks. In another group of diabetic rats, only saline was supplemented. After treatments, retinas from all the groups were isolated and analyzed for potential neurotrophic factors and apoptotic and oxidative stress markers using biochemical and immunoblotting techniques. Our results indicate that rutin possesses antidiabetic activity, as blood glucose level decreased and insulin level increased in diabetic rats. In the diabetic retina, rutin supplementation enhanced the reduced levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and glutathione (GSH) (P < 0.05), and reduced the level of thiobarbituric acid-reactive substances (TBARS) (P < 0.05). In addition, rutin treatment showed antiapoptotic activity by decreasing the level of caspase-3 and increasing the level of Bcl-2 in the diabetic retina. These results suggest the effectiveness of rutin in ameliorating the levels of neuroprotective factors in diabetic retina. Therefore, rutin might be a potential flavonoid that can prevent the retinal damage and subsequently the development of diabetic retinopathy.

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Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-VPP-179.

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Authors and Affiliations

  1. Department of Biochemistry, College of Science, King Saud University, Riyadh, 11415, Saudi Arabia

    Mohammad Shamsul Ola & Abdullah S. Alhomida

  2. Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, 11415, Saudi Arabia

    Mohammed M. Ahmed, Hatem M. Abuohashish & Salim S. Al-Rejaie

  3. Colorectal Research Center, College of Medicine, King Saud University, Riyadh, 11415, Saudi Arabia

    Rehan Ahmad

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  1. Mohammad Shamsul Ola
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  2. Mohammed M. Ahmed
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Correspondence to Mohammad Shamsul Ola.

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Ola, M.S., Ahmed, M.M., Ahmad, R. et al. Neuroprotective Effects of Rutin in Streptozotocin-Induced Diabetic Rat Retina. J Mol Neurosci 56, 440–448 (2015). https://doi.org/10.1007/s12031-015-0561-2

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