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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Beta-mangostin demonstrates apoptogenesis in murine leukaemia (WEHI-3) cells in vitro and in vivo

Authors: Fatima Abdelmutaal Ahmed Omer, Najihah Mohd Hashim, Mohamed Yousif Ibrahim, Abdulmannan F. Aldoubi, Pouya Hassandarvish, Firouzeh Dehghan, Noraziah Nordin, Hamed Karimian, Landa Zeenelabdin Ali Salim, Mahmood Ameen Abdulla, Karim Al-Jashamy, Syam Mohan

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Beta-mangostin (BM) is a xanthone-type of natural compound isolated from Cratoxylum arborescens. This study aimed to examine the apoptosis mechanisms induced by BM in a murine monomyelocytic cell line (WEHI-3) in vitro and in vivo.

Methods

A WEHI-3 cell line was used to evaluate the cytotoxicity of BM by MTT. AO/PI and Hoechst 33342 dyes, Annexin V, multiparametric cytotoxicity 3 by high content screening (HCS); cell cycle tests were used to estimate the features of apoptosis and BM effects. Caspase 3 and 9 activities, ROS, western blot for Bcl2, and Bax were detected to study the mechanism of apoptosis. BALB/c mice injected with WEHI-3 cells were used to assess the apoptotic effect of BM in vivo.

Results

BM suppressed the growth of WEHI-3 cells at an IC50value of 14 ± 3 μg/mL in 24 h. The ROS production was increased inside the cells in the treated doses. Both caspases (9 and 3) were activated in treating WEHI-3 cells at 24, 48 and 72 h. Different signs of apoptosis were detected, such as cell membrane blebbing, DNA segmentation and changes in the asymmetry of the cell membrane. Another action by which BM could inhibit WEHI-3 cells is to restrain the cell cycle at the G1/G0 phase. In the in vivo study, BM reduced the destructive effects of leukaemia on the spleen and liver by inducing apoptosis in leukaemic cells.

Conclusion

BM exerts anti-leukaemic properties in vitro and in vivo.
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Metadata
Title
Beta-mangostin demonstrates apoptogenesis in murine leukaemia (WEHI-3) cells in vitro and in vivo
Authors
Fatima Abdelmutaal Ahmed Omer
Najihah Mohd Hashim
Mohamed Yousif Ibrahim
Abdulmannan F. Aldoubi
Pouya Hassandarvish
Firouzeh Dehghan
Noraziah Nordin
Hamed Karimian
Landa Zeenelabdin Ali Salim
Mahmood Ameen Abdulla
Karim Al-Jashamy
Syam Mohan
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1867-0

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