Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Chemical constituents and coagulation activity of Agastache rugosa

Authors: Pengran Cao, Pingyao Xie, Xuebiao Wang, Jinmei Wang, Jinfeng Wei, Wen-yi Kang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Login to get access

Abstract

Background

In the Chinese traditional medicine, plant of Agastache rugosa (Fisch. & C.A. Mey.) Kuntze (A. rugosa) has been used to treat nausea, vomiting and dispel damp. However, currently, few reports about the chemical constituents, especially the non-volatile components of A. rugosa are available.

Methods

Through separation with various column chromatographies to elucidate the chemical constituents of A. rugosa, the biological activities of the major constituents were investigated. The extracts and main constituents of A. rugosa were evaluated for their anticoagulant effects by assaying the activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) in vitro.

Results

Seven known compounds (namely compounds 1–7) were isolated from the aerial parts of A. rugosa. They were identified as methyl hexadecanoate (1), β-sitosterol (2), acacetin (3), ursolic acid (4), apigenin (5), protocatechuic acid (6) and tilianin (7), respectively. Compounds 1 and 6 were isolated from the genus Agastache for the first time, and compound 4 was obtained from the plants for the first time. The results showed that the extract of A. rugosa had a significant procoagulant activity by shortening the time of PT (P < 0.001) and increasing FIB content (P < 0.001), as compared with Vitamin K1. While its major constituents acacetin and tilianin exhibited significant anticoagulant activities by prolonging the times of PT, APTT, TT and reducing FIB content (P < 0.001), as compared with blank control group.

Conclusions

The total extract of A. rugosa possessed significant procoagulant activity, while its main components, acacetin and tilianin possessed significant anticoagulant activities. Further investigation should be pursued to find out the bioactivity components responsible for the procoagulant action of the plant.
Literature
1.
Kim TH, Shin JH, Baek HH, Lee HJ. Volatile flavour compounds in suspension culture of Agastache rugosa Kuntze (Korean mint). J Sci Food Agr. 2001;81(6):569–75. View Article.CrossRef
2.
Kebede DT, Kim GS, Kim YH, Lee WS, Lee SJ, Jin JS, et al. The polyphenolic profiles and antioxidant effects of Agastache rugosa Kuntze (Banga) flower, leaf, stem and root. Biome Chromatogr. 2015;30(2):225–31 [View Article] [PubMed].
3.
Lee SY, Xu H, Kim YK, Park SU. Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze. World J Microb Biot. 2008;24(7):969–72 [View Article].CrossRef
4.
Li HQ, Liu QZ, Liu ZL, Du SS, Deng ZW. Chemical composition and nematicidal activity of essential oil of Agastache rugosa against Meloidogyne incognita. Molecules. 2013;18(4):4170–80 [View Article] [PubMed].CrossRefPubMed
5.
Shin S. Essential oil compounds from Agastache rugosa as antifungal agent against Trichophyton species. Arch Pharm Res. 2004;27(3):295–9 [PubMed].CrossRefPubMed
6.
Kim HK, Lee HK, Shin CG, Huh H. HIV integraseinhibitory activity of Agastache rugosa. Arch Pharm Res. 1999;22(5):520–3 [Article View] [PubMed].CrossRefPubMed
7.
Guo KJ, Xu SF, Yin P, Wang W, Song XZ, Liu FH, et al. Active components of common traditional Chinese medicine decoctions have antioxidant functions. J Anim Sci. 2011;89(10):3107–15 [PubMed].CrossRefPubMed
8.
Hernández-Abreu O, Torres-Piera M, García-Jiménez S, Ibarra-Barajas M, Villalobos-Molina R, Montes S, et al. Dose-dependent antihypertensive determination and toxicological studies of tilianin isolated from Agastache mexicana. J Ethnopharmacol. 2013;146(1):187–91 [View Article] [PubMed].CrossRefPubMed
9.
Guo X, Cao W, Yao J, Yuan Y, Hong Y, Wang X, et al. Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Mol Med Rep. 2015;11(3):2227–33 [View Article] [PubMed].PubMed
10.
Wang KC, Chang JS, Chiang LC, Lin CC. 4-Methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line. Phytomedicine. 2009;16(9):882–6 [View Article] [PubMed].CrossRefPubMed
11.
Zielińska S, Matkowski A. Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochem Rev. 2014;13(2):391–416 [View Article] [PubMed].CrossRefPubMedPubMedCentral
12.
Lee HK, Oh SR, Kim JI, Kim JW, Lee CO. Agastaquinone, a new cytotoxic diterpenoid quinone from Agastache rugosa. J Nat Prod. 1995;58(11):1718–21 [PubMed].CrossRefPubMed
13.
Tuan PA, Park WT, Xu H, Park NI, Park SU. Accumulation of tilianin and rosmarinic acid and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. J Agr Food Chem. 2012;60(23):5945–51 [PubMed].CrossRef
14.
Choi JH, Kim DW, Park SE, Lee HJ, Kim KM, Kim KJ, et al. Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille. J Biosci Bioeng. 2015;120(2):181–6 [View Article] [PubMed].CrossRefPubMed
15.
Rajlakshmi D, Sharma DK. Hypolipidemic effect of different extracts of Clerodendron colebrookianum Walp in normal and high-fat diet fed rats. J Ethnopharmacol. 2004;90(1):63–8 [View Article] [PubMed].CrossRef
16.
Chen H, Jin M, Wang YF, Wang YQ, Meng L, Li R, et al. Effect of Toona microcarpa Harms leaf extract on the coagulation system. Biomed Res Int. 2014;2014:22–32 [View Article] [PubMed].
17.
He F, Wang M, Gao M, Zhao M, Bai YH, Zhao CJ. Chemical composition and biological activities of Gerbera anandria. Molecules. 2014;19(4):4046–57 [View Article] [PubMed].CrossRefPubMed
18.
Ma ZK, Niu BJ, Zhang BB, Liao ZX. Chemical constituents from Pedicularis longiflora var. tubiformis. Chinese Traditional and Herbal Drugs. 2013;44(4):403–7 [View Article].
19.
Li HY, Jiao K, Zhang P, Gong SZ, Sun Y, Wu JZ. Chemical constituents from Isodon excisoides. Chin Tradit Herb Drugs. 2014;45(2):154–60 [View Article].
20.
Jin X, Shi SM, Zhang DF, Zhu Z. Chemical constituents of Andrographis paniculata (II). Chin Tradit Herb Drugs. 2014;45(2):164–9 [View Article].
21.
Xu LY, Li ZL, Cai ZC, Qian SH. Chemical constituents from Mentha haplocalyx. Chinese Traditional and Herbal Drugs. 2013;44(20):2798–802 [View Article].
22.
Li LT, Zhang X, Cui LL, Wang LN, Liu HC, Ji H, et al. Ursolic acid promotes the neuroprotection by activating Nrf2 pathway after cerebral ischemia in mice. Brain Res. 2013;1497:32–9 [PubMed].CrossRefPubMed
23.
Yang Y, Li CW, Xiang X, Dai ZL, Chang JY, Zhang M, et al. Ursolic acid prevents endoplasmic reticulum stress-mediated apoptosis induced by heat stress in mouse cardiac myocytes. J Mol Cell Cardiol. 2014;67:103–11 [View Article] [PubMed].CrossRefPubMed
24.
Li G, Wang H, Qin G, Jin M, Tang Q, Sun H, et al. Acacetin, a natural flavone, selectively inhibits human atrial repolarization potassium currents and prevents atrial fibrillation in dogs. Circulation. 2008;117(19):2449–57 [PubMed].CrossRefPubMed
25.
Hernández-Abreu O, Castillo-España P, León-Rivera I, Ibarra-Barajas M, Villalobos-Molina R, González-Christen J, et al. Antihypertensiveand vasorelaxant effects of tilianin isolated from Agastache Mexicana are mediated by NO/cGMP pathway and potassium channel opening. Biochem Pharmacol. 2009;78(1):54–61 [View Article] [PubMed].CrossRefPubMed
26.
Cho HI, Park JH, Choi HS, Kwak JH, Lee DU, Lee SK, et al. Protective mechanisms of acacetin against d-Galactosamine and lipopolysaccharide induced Fulminant Hepatic Failure in Mice. J Nat Prod. 2014;77(11):2497–503 [View Article] [PubMed].CrossRefPubMed
27.
Lee CH, Kim HN, Kho YE. Agastinol and agastenol, novel lignans from Agastache rugosa and their evaluation in an apoptosis inhibition assay. J Nat Prod. 2002;65(3):414–6 [View Article] [PubMed].CrossRefPubMed
28.
Itokawa H, Suto K, Takeya K. Structures of isoagastachoside and agastachin, new glucosylflavones isolated from Agastache rugosa. Chem Pharm Bull. 1981;29(6):1777–9 [View Article].CrossRef
29.
Zou ZM, Cong PZ. Studies on the chemical constituents from roots of Agastache rugosa. Acta Pharmacol Sin. 1991;26(12):906–10 [View Article] [PubMed].
30.
Ishitsuka H, Ohsawa C, Ohiwa T, Umeda I, Suhara Y. Antipicornavirus flavone Ro 09–0179. Antimicrob Agents Ch. 1982;22(4):611–6 [View Article] [PubMed].CrossRef
Metadata
Title
Chemical constituents and coagulation activity of Agastache rugosa
Authors
Pengran Cao
Pingyao Xie
Xuebiao Wang
Jinmei Wang
Jinfeng Wei
Wen-yi Kang
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1592-8

Other articles of this Issue 1/2017

BMC Complementary Medicine and Therapies 1/2017 Go to the issue

Research article

Antioxidant and vasorelaxant activities induced by northeastern Brazilian fermented grape skins

Research article

Xue-fu-Zhu-Yu decoction protects rats against retinal ischemia by downregulation of HIF-1α and VEGF via inhibition of RBP2 and PKM2

Research article

Maternal intake of dietary virgin coconut oil modifies essential fatty acids and causes low body weight and spiky fur in mice

Research article

"Placebo effect is probably what we refer to as patient healing power": A qualitative pilot study examining how Norwegian complementary therapists reflect on their practice

Research article

Anti-arthritic activity of ethanol extract of Claoxylon indicum on Freund’s complete adjuvant-induced arthritis in mice

Research article

A comparative assessment of antiproliferative properties of resveratrol and ethanol leaf extract of Anogeissus leiocarpus (DC) Guill and Perr against HepG2 hepatocarcinoma cells