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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

Authors: Wei Zou, Zuoqi Xiao, Xiaoke Wen, Jieying Luo, Shuqiong Chen, Zeneng Cheng, Daxiong Xiang, Jian Hu, Jingyu He

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism.

Methods

Thirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted.

Results

APN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway.

Conclusion

APN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.
Literature
1.
2.
Vicetti Miguel RD, Chivukula M, Krishnamurti U, Amortegui AJ, Kant JA, Sweet RL, Wiesenfeld HC, Phillips JM, Cherpes TL. Limitations of the criteria used to diagnose histologic endometritis in epidemiologic pelvic inflammatory disease research. Pathol res pract. 2011;207(11):680–5.CrossRefPubMedPubMedCentral
3.
Lee SA, Tsai HT, Ou HC, Han CP, Tee YT, Chen YC, Wu MT, Chou MC, Wang PH, Yang SF. Plasma interleukin-1beta, −6, −8 and tumor necrosis factor-alpha as highly informative markers of pelvic inflammatory disease. Clin chem lab med. 2008;46(7):997–1003.CrossRefPubMed
4.
Epstein FH, Barnes PJ, Karin M. Nuclear factor-κB-a pivotal transcription factor in chronic inflammatory diseases. N engl j med. 1997;336(15):1066–71.CrossRef
5.
Control CFD. Prevention: sexually transmitted diseases treatment guidelines, 2010. Ann emerg med. 2011;58(1):67–8.CrossRef
6.
Jayakumar T, Hsieh CY, Lee JJ, Sheu JR. Experimental and clinical pharmacology of andrographis paniculata and its major bioactive phytoconstituent andrographolide. Evid based complement alternat med. 2013;2013:846740.PubMedPubMedCentral
7.
SHARMA M, SHARMA R. Identification, purification and quantification of andrographolide from andrographis paniculata (burm. F.) nees by HPTLC at different stages of life cycle of crop. J curr chem pharm sci. 2013;3(1):23–32.
8.
Subramanian R, Asmawi MZ, Sadikun A. A bitter plant with a sweet future? a comprehensive review of an oriental medicinal plant: andrographis paniculata. Phytochem rev. 2012;11(1):39–75.CrossRef
9.
Zhou K-L, Chen L-X, Zhuang Y-L, Wang N-L, Yao X-S, Qiu F. Two new ent-labdane diterpenoid glycosides from the aerial parts of andrographis paniculata. J asian nat prod res. 2008;10(10):939–43.CrossRefPubMed
10.
Sareer O, Ahad A, Umar S. Prophylactic and lenitive effects of Andrographis paniculata against common human ailments: an exhaustive and comprehensive reappraisal. J Pharm Res Opin. 2014;2(10):138–62.
11.
Xu C, Chou G-X, Wang C-H, Wang Z-T. Rare noriridoids from the roots of andrographis paniculata. Phytochemistry. 2012;77:275–9.CrossRefPubMed
12.
Sheeja K, Kuttan G. Activation of cytotoxic T lymphocyte responses and attenuation of tumor growth in vivo by andrographis paniculata extract and andrographolide. Immunopharmacol immunotoxicol. 2007;29(1):81–93.CrossRefPubMed
13.
Rana A, Avadhoot Y. Hepatoprotective effects ofAndrographis paniculata against carbon tetrachloride-induced liver damage. Arch pharm res. 1991;14(1):93–5.CrossRefPubMed
14.
Chang RS, Ding L, Gai-Qing C, Qi-Choa P, Ze-Lin Z, Smith KM. Dehydroandrographolide succinic acid monoester as an inhibitor against the human immunodeficiency virus. Exp biol med. 1991;197(1):59–66.CrossRef
15.
Lin T-P, Chen S-Y, Duh P-D, Chang L-K, Liu Y-N. Inhibition of the Epstein-Barr virus lytic cycle by andrographolide. Biol pharm bull. 2008;31(11):2018–23.CrossRefPubMed
16.
Madav S, Tripathi H, Mishra S. Analgesic, antipyretic and antiulcerogenic effects of andrographolide. Indian j pharm sci. 1995;57(3):121.
17.
Kumar RA, Sridevi K, Kumar NV, Nanduri S, Rajagopal S. Anticancer and immunostimulatory compounds from andrographis paniculata. J ethnopharmacol. 2004;92(2):291–5.CrossRefPubMed
18.
Verma N, Vinayak M. Antioxidant action of andrographis paniculata on lymphoma. Mol biol rep. 2008;35(4):535–40.CrossRefPubMed
19.
Liu J, Wang Z-T, Ji L-L, Ge B-X. Inhibitory effects of neoandrographolide on nitric oxide and prostaglandin E2 production in LPS-stimulated murine macrophage. Mol cell biochem. 2007;298(1–2):49–57.CrossRefPubMed
20.
Voravuthikunchai SP, Limsuwan S. Medicinal plant extracts as anti-Escherichia coli O157: H7 agents and their effects on bacterial cell aggregation. J food protect. 2006;69(10):2336–41.CrossRef
21.
Xu Y, Marshall RL, Mukkur TK. An investigation on the antimicrobial activity of andrographis paniculata extracts and andrographolide in vitro. Asian j plant sci. 2006;5:527–30.CrossRef
22.
Zhang XF, Tan BKH. Antihyperglycaemic and anti‐oxidant properties of andrographis paniculata in normal and diabetic rats. Clin exp pharmacol physiol. 2000;27(5–6):358–63.CrossRefPubMed
23.
Zou W, Wen X, Zheng Y, Xiao Z, Luo J, Chen S, Wang Y, Cheng Z, Xiang D, Nie Y. Metabolomic study on the preventive effect of patrinia scabiosaefolia fisch on multipathogen induced pelvic inflammatory disease in rats. Evid based complement alternat med. 2015;501:170792.
24.
Luo YL, Zhang CC, Li PB, Nie YC, Wu H, Shen JG, Su WW. Naringin attenuates enhanced cough, airway hyperresponsiveness and airway inflammation in a guinea pig model of chronic bronchitis induced by cigarette smoke. Int immunopharmacol. 2012;13(3):301–7.CrossRefPubMed
25.
Jiang R, Wang H, Deng L, Hou J, Shi R, Yao M, Gao Y, Yao A, Wang X, Yu L, et al. IL-22 is related to development of human colon cancer by activation of STAT3. BMC cancer. 2013;13:59.CrossRefPubMedPubMedCentral
26.
World Health Organization. Global incidence and prevalence of selected curable sexually transmitted infections: 2008. vol. 20. In: World Health Organization, editor. Geneva: Reproductive Health Matters; 2012. p. 207–9.
27.
Quentin R, Verdon R. Microbiologic basis of diagnosis and treatment of pelvic inflammatory disease. J gynecol obstet biol reprod. 2012;41(8):850–63.CrossRef
28.
Saini S, Gupta N, Batra G, Arora DR. Role of anaerobes in acute pelvic inflammatory disease. Indian j med microbiol. 2003;21(3):189–92.PubMed
29.
Zhang D, Wen J, Zhou W, Wu X. Pathogenic bacteria distribution and drug resistance isolated from women with pelvic inflammatory disease. Chinese j nosocomiolog. 2009;19(13):1747–50.
30.
Pioli PA, Amiel E, Schaefer TM, Connolly JE, Wira CR, Guyre PM. Differential expression of toll-like receptors 2 and 4 in tissues of the human female reproductive tract. Infect immun. 2004;72(10):5799–806.CrossRefPubMedPubMedCentral
31.
He J, You X, Zeng Y, Yu M, Zuo L, Wu Y. Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-κB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway. Clin vaccine immunol. 2009;16(12):1750–7.CrossRefPubMedPubMedCentral
32.
Sheldon IM, Rycroft AN, Dogan B, Craven M, Bromfield JJ, Chandler A, Roberts MH, Price SB, Gilbert RO, Simpson KW. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. Plos one. 2010;5(2):e9192.CrossRefPubMedPubMedCentral
33.
Rebordão M, Carneiro C, Alexandre-Pires G, Brito P, Pereira C, Nunes T, Galvão A, Leitão A, Vilela C, Ferreira-Dias G. Neutrophil extracellular traps formation by bacteria causing endometritis in the mare. J reprod immunol. 2014;106:41–9.CrossRefPubMed
34.
Lv X, Fu K, Li W, Wang Y, Wang J, Li H, Tian W, Cao R. TIIA attenuates LPS-Induced mouse endometritis by suppressing the NF-κB signaling pathway. Can J Physiol Pharmacol. 2015;93(11):967–71.CrossRefPubMed
35.
Rank R, Soderberg L, Barron A. Chronic chlamydial genital infection in congenitally athymic nude mice. Infect immun. 1985;48(3):847–9.PubMedPubMedCentral
36.
Kiviat NB, Wølner-Hanssen P, Eschenbach DA, Wasserheit JN, Paavonen JA, Bell TA, Critchlow CW, Stamm WE, Moore DE, Holmes KK. Endometrial histopathology in patients with culture-proved upper genital tract infection and laparoscopically diagnosed acute salpingitis. Am j surg pathol. 1990;14(2):167–75.CrossRefPubMed
37.
Patton DL, Askienazy-Elbhar M, Henry-Suchet J, Campbell LA, Cappuccio A, Tannous W, Wang SP, Kuo CC. Detection of Chlamydia trachomatis in fallopian tube tissue in women with postinfectious tubal infertility. Am j obstet gynecol. 1994;171(1):95–101.CrossRefPubMed
38.
Tortorella C, Piazzolla G, Matteo M, Pinto V, Tinelli R, Sabbà C, Fanelli M, Cicinelli E. Interleukin-6, interleukin-1β, and tumor necrosis factor α in menstrual effluents as biomarkers of chronic endometritis. Fertil steril. 2014;101(1):242–7.CrossRefPubMed
39.
Cronin JG, Turner ML, Goetze L, Bryant CE, Sheldon IM. Toll-like receptor 4 and MYD88-dependent signaling mechanisms of the innate immune system are essential for the response to lipopolysaccharide by epithelial and stromal cells of the bovine endometrium. Biol reprod. 2012;86(2):51.CrossRefPubMedPubMedCentral
40.
Turner ML, Cronin JG, Healey GD, Sheldon IM. Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via toll-like receptors TLR2, TLR1, and TLR6. Endocrinology. 2014;155(4):1453–65.CrossRefPubMedPubMedCentral
41.
Gasperini S, Zambello R, Agostini C, Trentin L, Tassinari C, Cadrobbi P, Semenzato G, Cassatella MA. Impaired cytokine production by neutrophils isolated from patients with AIDS. Aids. 1998;12(4):373–9.CrossRefPubMed
42.
43.
Low M, Khoo CS, Munch G, Govindaraghavan S, Sucher NJ. An in vitro study of anti-inflammatory activity of standardised andrographis paniculata extracts and pure andrographolide. BMC complement altern med. 2015;15:18.CrossRefPubMedPubMedCentral
44.
Helenius M, Hänninen M, Lehtinen SK, Salminen A. Aging-induced up-regulation of nuclear binding activities of oxidative stress responsive NF-kB transcription factor in mouse cardiac muscle. J mol cell cardiol. 1996;28(3):487–98.CrossRefPubMed
45.
Ghosh S, May MJ, Kopp EB. NF-κB and Rel proteins: evolutionarily conserved mediators of immune responses. Annu rev immunol. 1998;16(1):225–60.CrossRefPubMed
46.
Bours V, Bonizzi G, Bentires-Alj M, Bureau F, Piette J, Lekeux P, Merville M-P. NF-κB activation in response to toxical and therapeutical agents: role in inflammation and cancer treatment. Toxicology. 2000;153(1):27–38.CrossRefPubMed
47.
Dai L, Gu L, Di W. MiR-199a attenuates endometrial stromal cell invasiveness through suppression of the IKKβ/NF-κB pathway and reduced interleukin-8 expression. Mol hum reprod. 2012;18(3):136–45.CrossRefPubMed
48.
Chao WW, Kuo YH, Hsieh SL, Lin BF. Inhibitory effects of ethyl acetate extract of andrographis paniculata on NF-kappaB trans-activation activity and LPS-induced acute inflammation in mice. Evid based complement alternat med. 2011;2011:254531.CrossRefPubMedPubMedCentral
49.
Parichatikanond W, Suthisisang C, Dhepakson P, Herunsalee A. Study of anti-inflammatory activities of the pure compounds from andrographis paniculata (burm.f.) nees and their effects on gene expression. Int immunopharmacol. 2010;10(11):1361–73.CrossRefPubMed
50.
Shao ZJ, Zheng XW, Feng T, Huang J, Chen J, Wu YY, Zhou LM, Tu WW, Li H. Andrographolide exerted its antimicrobial effects by upregulation of human beta-defensin-2 induced through p38 MAPK and NF-kappaB pathway in human lung epithelial cells. Can j physiol pharmacol. 2012;90(5):647–53.CrossRefPubMed
Metadata
Title
The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway
Authors
Wei Zou
Zuoqi Xiao
Xiaoke Wen
Jieying Luo
Shuqiong Chen
Zeneng Cheng
Daxiong Xiang
Jian Hu
Jingyu He
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1466-5

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