Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Evaluation of antioxidant, anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts

Authors: Tayyaba Afsar, Suhail Razak, Muhammad Rashid Khan, Saadia Mawash, Ali Almajwal, Maria Shabir, Ihsan Ul Haq

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Login to get access

Abstract

Background

Acacia hydaspica R. Parker, family leguminosae, is a medicinally important plant. Different plant parts are used in various ailments in folk medicine. The current study aimed at investigating the in vitro antioxidant, anti-hemolytic and anticancer activity of A. hydaspica.

Methods

Antioxidant potential was assessed using DPPH, ABTS and •OH, scavenging of H2O2, inhibition of lipid peroxidation and β-carotene bleaching inhibition assays. Anti-hemolytic activity was assessed using H2O2 induced hemolysis of RBCs. Anticancer potential was assessed using MTT assay. Spectrometric methods and HPLC-DAD analysis was performed for phytochemical screening.

Results

EC50 values based on reduction of DPPH, ABTS and •OH, scavenging of H2O2, inhibition of lipid peroxidation and β-carotene bleaching for AHB, AHE and AHM were generally lower manifesting potential antiradical capacities. The fractions also exhibited significant (P <0.001) anti-hemolytic potential. Regarding IC50 values for anticancer activity against HCC-38 and MDA-MB-361 cancer cell lines; AHB, AHE and AHM exhibited significant (P <0.001) cyto-selection indices. Plant extracts showed no cytotoxicity against normal Vero cells (IC50 > 250 μg/ml). While significant (P <0.001) cytotoxicity was elicited by these extract/fractions against cancer cell lines. AHE was the most effective and IC50 was found to be 29.9 ± 0.909 μg/ml (SI = 9.83) and 39.5 ± 0.872 μg/ml (SI = 7.44) against MDA-MB-361 and HCC-38 cancer cells respectively. Higher amounts of TPC and TFC were exhibited by AHE and AHB as compared to other fractions. Gallic acid, catechin and myricetin were identified in AHE whereas gallic acid and catechin were identified in AHB by HPLC.

Conclusion

The presence of bioactive constituents in AHE and AHB might be responsible for antioxidant, anti-hemolytic and anticancer activities.
Literature
1.
Mena S, Ortega A, Estrela J. Oxidative stress in environmental-induced carcinogenesis. Mutat Res Genet Toxicol Environ Mutagen. 2009;674(1):36–44.CrossRef
2.
3.
Sasaki Y, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S. The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat Res Genet Toxicol Environ Mutagen. 2002;519(1):103–19.CrossRef
4.
Soobrattee M, Bahorun T, Aruoma O. Chemopreventive actions of polyphenolic compounds in cancer. Biofactors. 2006;27(1–4):19–35.CrossRefPubMed
5.
Dai J, Mumper R. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15(10):7313–52.CrossRefPubMed
6.
Rj R, Cheng S-J, Je K. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese Green Tea. Carcinogenesis. 1989;10(6):1003–8.CrossRef
7.
Chakrabarty T, Gangopadhyay M. The genus Acacia P. Miller (Leguminosae: Mimosoideae) in India. J Econ Taxon Bot. 1996;20(3):599–633.
8.
Jabeen A, Khan Ma, Ahmad M, Zafar M, Ahmad F. Indigenous uses of economically important Flora of Margallah Hills National Park, Islamabad, Pakistan. African J Biotechnol. 2009;8(5):763–784.
9.
Afsar T, Khan M, Razak S, Ullah S, Mirza B. Antipyretic, anti-inflammatory and analgesic activity of Acacia Hydaspica R. Parker and its phytochemical analysis. BMC Complement Altern Med. 2015;15:136.CrossRefPubMedPubMedCentral
10.
Afsar T, Trembley J, Salomon C, Razak S, Khan M, Ahmed K. Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia Hydaspica: involvement of multiple signal transduction pathways. Sci Rep. 2016;6:23077.CrossRefPubMedPubMedCentral
11.
Malviya S, Rawat S, Kharia A, Verma M. International Journal of Pharmacy & Life Sciences. Int J Pharm Life Sci(Ijpls). 2011;2(6):830–7.
12.
Chang S-T, Wu J-H, Wang S-Y, Kang P-L, Yang N-S, Shyur L-F. Antioxidant activity of extracts from Acacia Confusa bark and heartwood. J Agric Food Chem. 2001;49(7):3420–4.CrossRefPubMed
13.
Mihara R, Barry K, Mohammed C, Mitsunaga T. Comparison of antifungal and antioxidant activities of Acacia Mangium and A. Auriculiformis heartwood extracts. J Chem Ecol. 2005;31(4):789–804.CrossRefPubMed
14.
Tung Y-T, Wu J-H, Huang C-Y, Kuo Y-H, Chang S-T. Antioxidant activities and phytochemical characteristics of extracts from Acacia Confusa Bark. Bioresour Technol. 2009;100(1):509–14.CrossRefPubMed
15.
Ghate N, Hazra B, Sarkar R, Mandal N. Heartwood extract of Acacia Catechu induces apoptosis in human breast carcinoma by altering Bax/Bcl-2 ratio. Pharmacogn Mag. 2014;10(37):27.CrossRefPubMedPubMedCentral
16.
Siddiqui A, Ali M. Practical pharmaceutical chemistry. Cbs Publishers Distrib New Delhi. 1997;126:131.
17.
Sofowora A. Recent trends in research into African medicinal plants. J Ethnopharmacol. 1993;38(2):197–208.CrossRef
18.
Trease G. Trease and Evans’ pharmacognosy. London: Bailliere Tindal; 1989.
19.
Talukdar A, Choudhury M, Chakraborty M, Dutta B. Phytochemical screening and tlc profiling of plant extracts of Cyathea Gigantea (Wall. Ex. Hook.) Haltt. and Cyathea Brunoniana. Wall. Ex. Hook (Cl. & Bak.). Assam Univ J Sci Technol. 2010;5(1):70–4.
20.
Harborne Jb. Phytochemical Methods. Springer. 1984;278:37-99.
21.
Mcdonald J, O’dwyer S, Rout S, Chakrabarty B, Sikand K, Fulford P, Wilson M, Renehan A. Classification of and Cytoreductive surgery for low-grade Appendiceal Mucinous Neoplasms. Br J Surg. 2012;99(7):987–92.CrossRefPubMed
22.
Chang C-C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10(3):178–82.
23.
Brand-Williams W, Cuvelier M, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Scie Technol. 1995;28(1):25–30.CrossRef
24.
Nishikimi M, Rao N, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun. 1972;46(2):849–54.CrossRefPubMed
25.
Gutteridge J, Halliwell B. Free radicals and antioxidants in the year 2000: a historical look to the future. Ann N Y Acad Sci. 2000;899(1):136–47.CrossRefPubMed
26.
Sahreen S, Khan M, Khan R. Evaluation of antioxidant activities of various solvent extracts of Carissa Opaca fruits. Food Chem. 2010;122(4):1205–11.CrossRef
27.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved Abts radical cation decolorization assay. Free Radic Biol Med. 1999;26(9):1231–7.CrossRefPubMed
28.
Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44(1):276–87.CrossRefPubMed
29.
Kishida E, Tokumaru S, Ishitani Y, Yamamoto M, Oribe M, Iguchi H, Kojo S. Comparison of the formation of malondialdehyde and thiobarbituric acid-reactive substances from autoxidized fatty acids based on oxygen consumption. J Agric Food Chem. 1993;41(10):1598–600.CrossRef
30.
Umamaheswari M, Chatterjee T. In vitro antioxidant activities of the fractions of Coccinia Grandis L. Leaf extract. Afr J Tradit Complement Altern Med. 2008;5(1):61–73.
31.
Kumaran A. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus Aromaticus. Food Chem. 2006;97(1):109–14.CrossRef
32.
Yang Z-G, Sun H-X, Fang W-H. Haemolytic activities and adjuvant effect of Astragalus Membranaceus Saponins (Ams) on the immune responses to ovalbumin in mice. Vaccine. 2005;23(44):5196–203.CrossRefPubMed
33.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1):55–63.CrossRefPubMed
34.
35.
Lu X-L, Qiu S-S, Sun X-X, Li Z-J. Preliminary study on the capability of antioxidation and scavenging free radicals of sasanquasaponins [J]. Food Sci. 2005;11:016.
36.
Beninger C, Hosfield G. Antioxidant activity of extracts, condensed tannin fractions, and pure flavonoids from Phaseolus Vulgaris L. Seed Coat color genotypes. J Agric Food Chem. 2003;51(27):7879–83.CrossRefPubMed
37.
Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004;74(17):2157–84.CrossRefPubMed
38.
Sangeetha S, Deepa M, Sugitha N, Mythili S, Sathiavelu A. Antioxidant activity and phytochemical analysis of Datura Metel. Int J Drug Dev Res. 2014;6(4):46–53.
39.
Korkina L, Ib Afanas’ E. Antioxidant and chelating properties of flavonoids. Adv Pharmacol. 1996;38:151–63.CrossRef
40.
Sultana B, Anwar F, Przybylski R. Antioxidant activity of phenolic components present in barks of Azadirachta Indica Terminalia Arjuna Acacia Nilotica and Eugenia Jambolana Lam. Trees Food Chem. 2007;104(3):1106–14.CrossRef
41.
Shah Na, Khan Mr, Naz K, Khan Ma. Antioxidant potential, Dna protection, and Hplc-dad analysis of neglected Medicinal Jurinea Dolomiaea roots. Biomed Res Int. 2014;2014:726241.
42.
Oi A. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat Res Fundam Mol Mech Mutagen. 2003;523:9–20.
43.
Singh R, Singh S, Kumar S, Arora S. Evaluation of antioxidant potential of Ethyl Acetate Extract/Fractions of Acacia Auriculiformis A. Cunn Food Chem Toxicol. 2007;45(7):1216–23.CrossRefPubMed
44.
Hochstein P, Atallah A. The nature of oxidants and antioxidant systems in the inhibition of mutation and cancer. Mutat Res Fundam Mol Mech Mutagen. 1988;202(2):363–75.CrossRef
45.
Gülçin İ, Oktay M, Küfrevioğlu Ö, Aslan A. Determination of antioxidant activity of Lichen Cetraria Islandica (L) ach. J Ethnopharmacol. 2002;79(3):325–9.CrossRefPubMed
46.
Khan R, Khan M, Sahreen S, Ahmed M. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea Procumbens. Chem Cent J. 2012;6(1):43.CrossRefPubMedPubMedCentral
47.
Chew Y-L, Goh J-K, Lim Y-Y. Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia. Food Chem. 2009;116(1):13–8.CrossRef
48.
Manian R, Anusuya N, Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia Sinensis (L.) O. Kuntz, Ficus Bengalensis L. and Ficus Racemosa L. Food Chem. 2008;107(3):1000–7.CrossRef
49.
Kartal N, Sokmen M, Tepe B, Daferera D, Polissiou M, Sokmen A. Investigation of the antioxidant properties of Ferula Orientalis L. using a suitable extraction procedure. Food Chem. 2007;100(2):584–9.CrossRef
50.
Barros L, Ferreira M-J, Queiros B, Ferreira I, Baptista P. Total phenols, ascorbic acid, B-Carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem. 2007;103(2):413–9.CrossRef
51.
Khan R, Khan M, Sahreen S, Ahmed M. Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus Asper (L.) hill. Chem Cent J. 2012;6(12):1–7.
52.
Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium Polium L. Food Chem. 2009;112(4):885–8.CrossRef
53.
Tung Y-T, Wu J-H, Hsieh C-Y, Chen P-S, Chang S-T. Free radical-scavenging phytochemicals of hot water extracts of Acacia Confusa leaves detected by an on-line screening method. Food Chem. 2009;115(3):1019–24.CrossRef
54.
Fejes S, Blázovics A, Lugasi A, Lemberkovics É, Petri G, Kéry Á. In vitro antioxidant activity of Anthriscus Cerefolium L. (Hoffm.) extracts. J Ethnopharmacol. 2000;69(3):259–65.CrossRefPubMed
55.
Ebrahimzadeh M, Nabavi S, Nabavi S. Antioxidant activities of methanol extract of Sambucus Ebulus L. Flower Pak J Biol Sci. 2009;12(5):447.CrossRefPubMed
56.
57.
Naim M, Gestetner B, Bondi A, Birk Y. Antioxidative and antihemolytic activities of soybean isoflavones. J Agric Food Chem. 1976;24(6):1174–7.CrossRefPubMed
58.
Kalaivani T, Rajasekaran C, Suthindhiran K, Mathew L. Free radical scavenging, cytotoxic and hemolytic activities from leaves of Acacia Nilotica (L.) Wild. Ex. Delile Subsp. Indica (Benth.) Brenan. Evid Based Complement Alternat Med. 2011;2011.
59.
Sghaier M, Skandrani I, Nasr N, Franca M-G, Chekir-Ghedira L, Ghedira K. Flavonoids and sesquiterpenes from Tecurium Ramosissimum promote antiproliferation of human cancer cells and enhance antioxidant activity: a structure-activity relationship study. Environ Toxicol Pharmacol. 2011;32(3):336–48.CrossRefPubMed
60.
Li N, Liu J-H, Zhang J, Yu B-Y. Comparative evaluation of cytotoxicity and antioxidative activity of 20 flavonoids. J Agric Food Chem. 2008;56(10):3876–83.CrossRefPubMed
61.
62.
Obrenovich M, Nair N, Beyaz A, Aliev G, Reddy V. The role of polyphenolic antioxidants in health, disease, and aging. Rejuvenation Res. 2010;13(6):631–43.CrossRefPubMed
63.
Ali A, Akhtar N, Khan B, Khan M, Rasul A, Zaman S, Khalid N, Waseem K, Mahmood T, Ali L. Acacia Nilotica: a plant of multipurpose medicinal uses. J Med Plant Res. 2012;6:1492–6.
64.
Gaascht F, Dicato M, Diederich M. Venus Flytrap (Dionaea Muscipula Solander Ex Ellis) contains powerful compounds that prevent and cure cancer. Front Oncol 2013;3.
Metadata
Title
Evaluation of antioxidant, anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts
Authors
Tayyaba Afsar
Suhail Razak
Muhammad Rashid Khan
Saadia Mawash
Ali Almajwal
Maria Shabir
Ihsan Ul Haq
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1240-8

Other articles of this Issue 1/2016

BMC Complementary Medicine and Therapies 1/2016 Go to the issue

Research article

Melastoma malabathricum Linn attenuates complete freund’s adjuvant-induced chronic inflammation in Wistar rats via inflammation response

Research article

Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Research article

Rhodiola crenulata extract regulates hepatic glycogen and lipid metabolism via activation of the AMPK pathway

Research article

Antiplatelet and antithrombotic effects of cordycepin-enriched WIB-801CE from Cordyceps militaris ex vivo, in vivo, and in vitro

Research article

An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills for pulmonary diseases

Research article

Protective effects of saffron extract and crocin supplementation on fatty liver tissue of high-fat diet-induced obese rats