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BMC Complementary Medicine and Therapies

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Open Access 01-12-2016 | Research article

Protective effect of 3-O-methyl quercetin and kaempferol from Semecarpus anacardium against H₂O₂ induced cytotoxicity in lung and liver cells

Authors: A. D. Naveen Kumar, Ganesh Babu Bevara, Laxmi Koteswaramma Kaja, Anil Kumar Badana, Rama Rao Malla

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Abstract

Background

Hydrogen peroxide is continuously generated in living cells through metabolic pathways and serves as a source of reactive oxygen species. Beyond the threshold level, it damages cells and causes several human disorders, including cancer.

Methods

Effect of isolated 3-O-methyl quercetin and kaempferol on H₂O₂ induced cytotoxicity, ROS formation, plasma membrane damage, loss of mitochondrial membrane potential, DNA damage was evaluated in normal liver and lung cells. The RT-PCR analysis used to determine Nrf 2 gene expression. Calorimetric ELISA was used to determine Nrf2 and p-38 levels. Expression of SOD and catalase was analyzed by Western blot analysis.

Results

The present study isolated 3-O-methyl quercetin and kaempferol from the stem bark. They protected normal lung and liver cells from H₂O₂ induced cytotoxicity, ROS formation, membrane damage and DNA damage. Pre-treatment with 3-O-methyl quercetin and kaempferol caused translocation of Nrf2 from cytosol to nucleus. It also increased expression of p-p38, Nrf2, SOD and catalase in H₂O₂ treated lung and liver cells.

Conclusion

The flavonoids isolated from S. anacardium significantly reduced H₂O₂ induced stress and increased expression of Nrf2, catalase and superoxide dismutase-2 indicating cytoprotective nature of 3-O-methylquercetin and kaempferol.

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Title: Protective effect of 3-O-methyl quercetin and kaempferol from Semecarpus anacardium against H2O2 induced cytotoxicity in lung and liver cells
Authors: A. D. Naveen Kumar
Ganesh Babu Bevara
Laxmi Koteswaramma Kaja
Anil Kumar Badana
Rama Rao Malla
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1354-z

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Protective effect of 3-O-methyl quercetin and kaempferol from Semecarpus anacardium against H₂O₂ induced cytotoxicity in lung and liver cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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