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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice

Authors: Shuangshuang Zhang, Xiangdong Liu, Lihong Mei, Hongfeng Wang, Fang Fang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Psoriasis is a chronic inflammatory immune disease with undefined pathogenesis. It is associated with T cells, and the IL-23/IL17 axis is believed to be crucial in the pathogenesis. The present treatments have side effects that influence the compliance of patients. Tea polyphenol is extracted from tea polyphenols, and its main active ingredient is Epigallocatechin-3-gallate (EGCG), which has been shown to have antioxidant, anti-tumor, and anti-ultraviolet radiation effects. Here, we aim to report that EGCG can inhibit imiquimod (IMQ)-induced psoriasis-like inflammation.

Methods

We used BALB/c mice, which were topically treated with IMQ for 6 consecutive days, as a psoriasis mouse model. Topical application of EGCG and treatment with EGCG were conducted in the experiments. Then observed the effects of the two methods on psoriasis-like mice dermatitis. Statistics are presented as the means ± standard error of mean (SEM) and compared using unpaired two-tailed Student’s t tests or one-way ANOVA.

Results

Topical application of EGCG alleviated psoriasiform dermatitis, improved the skin pathological structure by reduce the expression of epidermal PCNA, promoted the expression of caspase-14. Treatment with EGCG attenuated skin inflammation, accompanied by reduced infiltrations of T cells; reduced percentages of CD11c+ DC in the composition of immunocytes of spleens; reduced levels of interleukin (IL)-17A, IL-17F, IL-22, IL-23 and malondialdehyde (MDA) in plasma; increased percentages of CD4+ T cells in the composition of immunocytes of spleens; and increased bioactivities of superoxide dismutase (SOD) and catalase (CAT) in plasma.

Conclusions

All the results demonstrated that EGCG had anti-inflammatory, immune regulatory and antioxidant effects. It is a promising intervention in psoriasis in the future.
Literature
1.
Martin DA, Towne JE, Kricorian G, et al. The emerging role of IL-17 in the pathogenesis of psoriasis: preclinical and clinical findings. J Invest Dermatol. 2013;133(1):17–26.CrossRefPubMed
2.
Weidemann AK, Crawshaw AA, Byrne E, et al. Vascular endothelial growth factor inhibitors: investigational therapies for the treatment of psoriasis. Clin Cosmet Investig Dermatol. 2013;6:233–44.PubMedPubMedCentral
3.
Sun J, Zhao Y, Hu J. Curcumin inhibits imiquimod-induced psoriasis-like inflammation by inhibiting IL-1beta and IL-6 production in mice. PLoS One. 2013;8(6), e67078.CrossRefPubMedPubMedCentral
4.
Qin S, Wen J, Bai XC, et al. Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis. Mol Med Rep. 2014;9(6):2097–104.PubMedPubMedCentral
5.
Ha HL, Wang H, Pisitkun P, et al. IL-17 drives psoriatic inflammation via distinct, target cell-specific mechanisms. Proc Natl Acad Sci. 2014;111(33):E3422–31.CrossRefPubMedPubMedCentral
6.
Witte E, Kokolakis G, Witte K, et al. IL-19 Is a Component of the Pathogenetic IL-23/IL-17 Cascade in Psoriasis. J Invest Dermatol. 2014;134(11):2757–67.CrossRefPubMed
7.
Van Belle AB, de Heusch M, Lemaire MM, et al. IL-22 is required for imiquimod-induced psoriasiform skin inflammation in mice. J Immunol. 2012;188(1):462–9.CrossRefPubMed
8.
Croxford AL, Karbach S, Kurschus FC, et al. IL-6 regulates neutrophil microabscess formation in IL-17A-driven psoriasiform lesions. J Invest Dermatol. 2014;134(3):728–35.CrossRefPubMed
9.
Kirkham BW, Kavanaugh A, Reich K. Interleukin-17A: a unique pathway in immune-mediated diseases: psoriasis, psoriatic arthritis and rheumatoid arthritis. Immunology. 2014;141(2):133–42.CrossRefPubMedPubMedCentral
10.
11.
Baek J, Byamba D, Wu WH, et al. Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress. Arch Dermatol Res. 2012;304(9):699–706.CrossRefPubMed
12.
Wong T, Hsu L, Liao W. Phototherapy in psoriasis: a review of mechanisms of action. J Cutan Med Surg. 2013;17(1):6–12.CrossRefPubMed
13.
Dubois DS, Pouliot R. Promising new treatments for psoriasis. ScientificWorldJournal. 2013;2013:980419.
14.
Villasenor-Park J, Wheeler D, Grandinetti L. Psoriasis: evolving treatment for a complex disease. Cleve Clin J Med. 2012;79(6):413–23.CrossRefPubMed
15.
Han R, Rostami-Yazdi M, Gerdes S, et al. Triptolide in the treatment of psoriasis and other immune-mediated inflammatory diseases. Br J Clin Pharmacol. 2012;74(3):424–36.CrossRefPubMedPubMedCentral
16.
Jung MK, Ha S, Son JA, et al. Polyphenon-60 displays a therapeutic effect on acne by suppression of TLR2 and IL-8 expression via down-regulating the ERK1/2 pathway. Arch Dermatol Res. 2012;304(8):655–63.CrossRefPubMed
17.
Ellis LZ, Liu W, Luo Y, et al. Green tea polyphenol epigallocatechin-3-gallate suppresses melanoma growth by inhibiting inflammasome and IL-1beta secretion. Biochem Biophys Res Commun. 2011;414(3):551–6.CrossRefPubMedPubMedCentral
18.
Meeran SM, Akhtar S, Katiyar SK. Inhibition of UVB-induced skin tumor development by drinking green tea polyphenols is mediated through DNA repair and subsequent inhibition of inflammation. J Invest Dermatol. 2009;129(5):1258–70.CrossRefPubMed
19.
Katiyar SK, Afaq F, Perez A, et al. Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress. Carcinogenesis. 2001;22(2):287–94.CrossRefPubMed
20.
Shankar S, Chen Q, Srivastava RK. Inhibition of PI3K/AKT and MEK/ERK pathways act synergistically to enhance antiangiogenic effects of EGCG through activation of FOXO transcription factor. J Mol Signal. 2008;3:7.CrossRefPubMedPubMedCentral
21.
Bickers DR, Athar M. Novel approaches to chemoprevention of skin cancer[J]. J Dermatol. 2000;27(11):691–95.CrossRefPubMed
22.
Schonthaler HB, Huggenberger R, Wculek SK, et al. Systemic anti-VEGF treatment strongly reduces skin inflammation in a mouse model of psoriasis[J]. Proc Natl Acad Sci USA. 2009;106(50):21264–9.CrossRefPubMedPubMedCentral
23.
van der Fits L, Mourits S, Voerman JSA, et al. Imiquimod-Induced Psoriasis-Like Skin Inflammation in Mice Is Mediated via the IL-23/IL-17 Axis. J Immunol. 2009;182(9):5836–45.CrossRefPubMed
24.
Kim HR, Lee A, Choi EJ, et al. Reactive oxygen species prevent imiquimod-induced psoriatic dermatitis through enhancing regulatory T cell function. PLoS One. 2014;9(3), e91146.CrossRefPubMedPubMedCentral
25.
Glitzner E, Korosec A, Brunner PM, et al. Specific roles for dendritic cell subsets during initiation and progression of psoriasis. EMBO Mol Med. 2014;6(10):1312–27.CrossRefPubMedPubMedCentral
26.
27.
Akarsu S, Aktan Ş, Atahan A, et al. Comparison of topical 3 % diclofenac sodium gel and 5 % imiquimod cream for the treatment of actinic keratoses. Clin Exp Dermatol. 2011;36(5):479–84.CrossRefPubMed
28.
Garcia-Martin E, Idoipe M, Gil LM, et al. Efficacy and Tolerability of Imiquimod 5 % Cream to Treat Periocular Basal Cell Carcinomas. J Ocul Pharmacol Ther. 2010;26(4):373–9.CrossRefPubMed
29.
Heinrich U, Moore CE, De Spirt S, et al. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women. J Nutr. 2011;141(6):1202–8.CrossRefPubMed
30.
Hsu S, Dickinson D, Borke J, et al. Green tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model. Exp Dermatol. 2007;16(8):678–84.CrossRefPubMed
31.
Pal HC, Chamcheu JC, Adhami VM, et al. Topical application of delphinidin reduces psoriasiform lesions in the flaky skin mouse model by inducing epidermal differentiation and inhibiting inflammation. Br J Dermatol. 2015;172(2):354–64.CrossRefPubMed
32.
Hoste E, Denecker G, Gilbert B, et al. Caspase-14-deficient mice are more prone to the development of parakeratosis. J Invest Dermatol. 2013;133(3):742–50.CrossRefPubMed
33.
Lima EA, Lima MA. Reviewing concepts in the immunopathogenesis of psoriasis. An Bras Dermatol. 2011;86(6):1151–8.
34.
Coimbra S, Figueiredo A, Castro E, et al. The roles of cells and cytokines in the pathogenesis of psoriasis. Int J Dermatol. 2012;51(4):389–98.CrossRefPubMed
35.
Ikonomidis I, Makavos G, Papadavid E, et al. Similarities in coronary function and myocardial deformation between psoriasis and coronary artery disease: the role of oxidative stress and inflammation. Can J Cardiol. 2015;31(3):287–95.CrossRefPubMed
36.
Gabr SA, Al-Ghadir AH. Role of cellular oxidative stress and cytochrome c in the pathogenesis of psoriasis. Arch Dermatol Res. 2012;2012(304):451–7.CrossRef
37.
Chen H, Zhang Y, Lu X, et al. Comparative studies on the physicochemical and antioxidant properties of different tea extracts. J Food Sci Technol. 2012;49(3):356–61.CrossRefPubMed
38.
Barreira JC, Morais AL, Ferreira IC, et al. Insights on the formulation of herbal beverages with medicinal claims according with their antioxidant properties. Molecules. 2013;18(3):2851–63.CrossRefPubMed
39.
Kim HS, Quon MJ, Kim JA. New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate. Redox Biol. 2014;2(2014):187–95.CrossRefPubMedPubMedCentral
Metadata
Title
Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice
Authors
Shuangshuang Zhang
Xiangdong Liu
Lihong Mei
Hongfeng Wang
Fang Fang
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1325-4

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