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Archives of Dermatological Research
Published in: Archives of Dermatological Research 9/2012

01-11-2012 | Original Paper

Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress

Authors: Jin-Ok Baek, Dashlkhumbe Byamba, Wen Hao Wu, Tae-Gyun Kim, Min-Geol Lee

Published in: Archives of Dermatological Research | Issue 9/2012

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Abstract

Psoriasis is a chronic inflammatory skin disease that is thought to be related to oxidative stress. Much progress has been made in understanding the pathophysiology of psoriasis in relation to the immunologic and antioxidant systems. However, this progress has been hindered by the lack of an appropriate animal model for psoriasis. Recently, imiquimod (IQM)-induced psoriasis-like cutaneous inflammation has been reported in mice and humans. We verified the usefulness of an IQM-induced mouse model in relation to the antioxidant system. BALB/C female mice at 8–10 weeks of age were treated with IQM cream in this study. We analyzed clinical and histopathological changes. Increased reactive oxygen species production was measured by glutathione assay. Levels of myeloperoxidase (MPO) and superoxide dismutase-1 (SOD1) were determined by western blotting and immunohistochemical analyses. The activity of SOD was measured by a SOD activity assay kit. Application of IQM-induced skin inflammation similar to psoriasis in clinical and histopathological aspects. Accumulation of immune cells was confirmed. Oxidative stress was increased, the antioxidant enzyme MPO levels were increased, and both SOD levels and activity were decreased. In conclusion, the IQM-induced mouse model showed an aberrant antioxidant system. Levels of MPO and oxidative stress were increased, and the level and activity of SOD were decreased. Since this model seemed to be an appropriate model for psoriasis, it can be used to further study the pathogenic role of redox imbalance in psoriasis.
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Metadata
Title
Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress
Authors
Jin-Ok Baek
Dashlkhumbe Byamba
Wen Hao Wu
Tae-Gyun Kim
Min-Geol Lee
Publication date
01-11-2012
Publisher
Springer-Verlag
Published in
Archives of Dermatological Research / Issue 9/2012
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI
https://doi.org/10.1007/s00403-012-1272-y

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