Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

ACC 2024 Congress

Catch up on all the top stories and latest evidence in cardiology research with our ACC 2024 Congress hub page.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Salvianolic acid B induced upregulation of miR-30a protects cardiac myocytes from ischemia/reperfusion injury

Authors: Dan Li, Jun Wang, Jincai Hou, Jianhua Fu, Jianxun Liu, Ruichao Lin

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Login to get access

Abstract

Background

MicroRNAs (miRNAs) are a novel class of powerful, endogenous regulators of gene expression. This study was designed to ascertain if miR-30a is involved in the cardioprotective actions of salvianolic acid B (Sal B) against myocardial ischemia–reperfusion (I–R) injury through suppression of autophagy.

Methods

Murine myocardial cells that had undergone primary culture were induced by I–R and incubated with Sal B (25, 50, 100 μM) in the presence of a miR-30a mimic or miR-30a inhibitor. Expression of miR-30a, beclin-1, LC3-II and p-Akt protein, cell viability, and lactic acid dehydrogenase (LDH) release were assessed.

Results

miR-30a expression was down-regulated remarkably in I–R cells, and this suppression could be reversed by Sal B in a dose-dependent manner. Sal B repressed autophagy in I–R myocardial cells. Sal B improved cell viability and reduced the rate of LDH leakage, which suggested that autophagy suppression was beneficial for cell survival. Knockdown of miR-30a with a miR-30a inhibitor could reverse the anti-autophagy effect of Sal B against I–R injury. Furthermore, we confirmed that Sal B has a protective role in miR-30a-mediated autophagy through the PI3K/Akt signaling pathway, which was abrogated by the PI3K inhibitor LY294002.

Conclusions

These data suggest that miR-30a is involved in Sal B-mediated cardioprotection against I–R injury through the PI3K/Akt signaling pathway.
Appendix
Available only for authorised users
Literature
1.
Forouzanfar MH, Moran AE, Flaxman AD, Roth G, Mensah GA, Ezzati M, et al. Assessing the global burden of ischemic heart disease, part 2: analytic methods and estimates of the global epidemiology of ischemic heart disease in 2010. Global Heart. 2012;7(4):331–42.CrossRefPubMedPubMedCentral
2.
3.
4.
Sadamatsu K, Koide S, Nakano K, Yoshida K. Heart rate control with single administration of a long-acting beta-blocker at bedtime before coronary computed tomography angiography. J Cardiogr. 2015;65(4):293–7.CrossRef
5.
Sharma KK, Mathur M, Gupta R, Guptha S, Roy S, Khedar RS, et al. Epidemiology of cardioprotective pharmacological agent use in stable coronary heart disease. Indian Heart J. 2013;65(3):250–5.CrossRefPubMedPubMedCentral
6.
Shen Y, Shen Z, Miao L, Xin X, Lin S, Zhu Y, et al. miRNA-30 family inhibition protects against cardiac ischemic injury by regulating Cystathionine-gamma-lyase expression. Antioxid Redox Signal. 2015;22(3):224–40.CrossRefPubMedPubMedCentral
7.
Yang Y, Li Y, Chen X, Cheng X, Liao Y, Yu X. Exosomal transfer of miR-30a between c ardiomyocytes regulates autophagy after hypoxia. J Mol Med. 2016;94(6):711-24.
8.
Pan W, Zhong Y, Cheng C, Liu B, Wang L, Li A. MiR-30-Regulated Autophagy Mediates Angiotensin II-Induced Myocardial Hypertrophy. PLoS One. 2013;8(1):e53950.CrossRefPubMedPubMedCentral
9.
Francesca F, Claudia K, Giuseppina N, Laura M, Riccardo Z, Marco M, et al. Triiodothyronine Prevents cardiac ischemia/reperfusion mitochondrial impairment and cell loss by regulating miR30a/p53 Axis. Endocrinology. 2014;155(11):4581–90.CrossRef
10.
11.
Matsui Y, Takagi H, Qu X, Abdellatif M, Sakoda H, Asano T, et al. Distinct roles of autophagy in the heart during ischemia and reperfusion: roles of AMP-activated protein kinase and Beclin 1 in mediating autophagy. Circ Res. 2007;100(6):914–22.CrossRefPubMed
12.
Decker RS, Wildenthal K. Lysosomal alterations in hypoxic and reoxygenated hearts. I. Ultrastructural and cytochemical changes. Am J Pathol. 1980;98(2):425–44.PubMedPubMedCentral
13.
Huang CQ, Yitzhaki S, Perry CN, Liu WN, Giricz Z, Mentzer RM, et al. Autophagy induced by ischemic preconditioning is essential for cardioprotection. J Cardiovasc Transl Res. 2010;3(4):365–73.CrossRefPubMedPubMedCentral
14.
Jahania SM, Sengstock D, Vaitkevicius P, Andres A, Ito BR, Gottlieb RA, et al. Activation of the homeostatic intracellular repair response during cardiac surgery. J Am Coll Surg. 2013;216(4):719–26. discussion 726-719.CrossRefPubMedPubMedCentral
15.
Kassiotis C, Ballal K, Wellnitz K, Vela D, Gong M, Salazar R, et al. Markers of autophagy are downregulated in failing human heart after mechanical unloading. Circulation. 2009;120(11 Suppl):S191–197.CrossRefPubMedPubMedCentral
16.
Zhu H, Wu H, Liu X, Li B, Chen Y, Ren X, et al. Regulation of autophagy by a beclin 1-targeted microRNA, miR-30a, in cancer cells. Autophagy. 2009;5(6):816–23.CrossRefPubMedPubMedCentral
17.
Yao H, Han X, Han X. The cardioprotection of the insulin-mediated PI3K/Akt/mTOR signaling pathway. Am J Cardiovasc Drugs. 2014;14(6):433–42.CrossRefPubMed
18.
Hu G, Huang X, Zhang K, Jiang H, Hu X. Anti-inflammatory effect of B-Type natriuretic peptide postconditioning during myocardial ischemia-reperfusion: involvement of PI3K/Akt signaling pathway. Inflammation. 2014;37(5):1669–74.CrossRefPubMed
19.
Zhong M, Bian ZQ, Wu ZY. miR-30a suppresses cell migration and invasion through downregulation of PIK3CD in colorectal carcinoma. Cell Physiol Biochem. 2013;31(2-3):209–18.CrossRefPubMed
20.
Tanida I. Autophagosome formation and molecular mechanism of autophagy. Antioxid Redox Signal. 2011;14(11):2201–14.CrossRefPubMed
21.
Joe Y, Zheng M, Kim HJ, Kim S, Uddin MJ, Park C, et al. Salvianolic acid B exerts vasoprotective effects through the modulation of heme oxygenase-1 and arginase activities. J Pharmacol Exp Ther. 2012;341(3):850–8.CrossRefPubMed
22.
Jiang B, Chen J, Xu L, Gao Z, Deng Y, Wang Y, et al. Salvianolic acid B functioned as a competitive inhibitor of matrix metalloproteinase-9 and efficiently prevented cardiac remodeling. BMC Pharmacol. 2010;10:10.CrossRefPubMedPubMedCentral
23.
Yao Y, Wu WY, Liu AH, Deng SS, Bi KS, Liu X, et al. Interaction of salvianolic acids and notoginsengnosides in inhibition of ADP-induced platelet aggregation. Am J Chin Med. 2008;36(2):313–28.CrossRefPubMed
24.
Chen YH, Lin SJ, Ku HH, Shiao MS, Lin FY, Chen JW, et al. Salvianolic acid B attenuates VCAM-1 and ICAM-1 expression in TNF-alpha-treated human aortic endothelial cells. J Cell Biochem. 2001;82(3):512–21.CrossRefPubMed
25.
Han X, Liu JX, Li XZ. Salvianolic acid B inhibits autophagy and protects starving cardiac myocytes. Acta Pharmacol Sin. 2011;32(1):38–44.CrossRefPubMed
26.
Wu YJ, Hong CY, Lin SJ, Wu P, Shiao MS. Increase of vitamin E content in LDL and reduction of atherosclerosis in cholesterol-fed rabbits by a water-soluble antioxidant-rich fraction of Salvia miltiorrhiza. Arterioscler Thromb Vasc Biol. 1998;18(3):481–6.CrossRefPubMed
27.
Ding M, Yuan YJ. Study on the mechanisms of an extract of Salvia miltiorrhiza on the regulation of permeability of endothelial cells exposed to tumour necrosis factor-alpha. J Pharm Pharmacol. 2007;59(7):1027–33.CrossRefPubMed
28.
Chen YL, Hu CS, Lin FY, Chen YH, Sheu LM, Ku HH, et al. Salvianolic acid B attenuates cyclooxygenase-2 expression in vitro in LPS-treated human aortic smooth muscle cells and in vivo in the apolipoprotein-E-deficient mouse aorta. J Cell Biochem. 2006;98(3):618–31.CrossRefPubMed
29.
30.
Lew WY, Bayna E, Dalle Molle E, Contu R, Condorelli G, Tang T. Myocardial fibrosis induced by exposure to subclinical lipopolysaccharide is associated with decreased miR-29c and enhanced NOX2 expression in mice. PloS One. 2014;9(9):e107556.CrossRefPubMedPubMedCentral
31.
Song CL, Liu B, Diao HY, Shi YF, Li YX, Zhang JC, et al. The protective effect of MicroRNA-320 on left ventricular remodeling after myocardial ischemia-reperfusion injury in the rat model. Int J Mol Sci. 2014;15(10):17442–56.CrossRefPubMedPubMedCentral
32.
Huang J, Huang C, Luo Y, Liu S, Chen X. Role of MiR-30a in cardiomyocyte autophagy induced by Angiotensin II. J Renin Angiotensin Aldosterone Syst. 2015;16(1):1–5.CrossRefPubMed
33.
Long G, Wang F, Duan Q, Yang S, Chen F, Gong W, et al. Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. PloS One. 2012;7(12):e50926.CrossRefPubMedPubMedCentral
34.
Wang P, Liang J, Li Y, Li JF, Yang X, Zhang XX, et al. Down-Regulation of miRNA-30a Alleviates Cerebral Ischemic Injury Through Enhancing Beclin 1-Mediated Autophagy. Neurochem Res. 2014;39(7):1279–91.CrossRefPubMed
35.
Aita VM, Liang XH, Murty VV, Pincus DL, Yu W, Cayanis E, et al. Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics. 1999;59(1):59–65.CrossRefPubMed
36.
Liang XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature. 1999;402(6762):672–6.CrossRefPubMed
37.
Qu X, Yu J, Bhagat G, Furuya N, Hibshoosh H, Troxel A, et al. Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J Clin Invest. 2003;112(12):1809–20.CrossRefPubMedPubMedCentral
38.
Valentim L, Laurence KM, Townsend PA, Carroll CJ, Soond S, Scarabelli TM, et al. Urocortin inhibits Beclin1-mediated autophagic cell death in cardiac myocytes exposed to ischaemia/reperfusion injury. J Mol Cell Cardiol. 2006;40(6):846–52.CrossRefPubMed
39.
40.
Pan H, Li D, Fang F, Chen D, Qi L, Zhang R, et al. Salvianolic acid A demonstrates cardioprotective effects in rat hearts and cardiomyocytes after ischemia/reperfusion injury. J Cardiovasc Pharmacol. 2011;58(5):535–42.CrossRefPubMed
41.
Zhang J, Liu A, Hou R, Zhang J, Jia X, Jiang W, et al. Salidroside protects cardiomyocyte against hypoxia-induced death: a HIF-1alpha-activated and VEGF-mediated pathway. Eur J Pharmacol. 2009;607(1-3):6–14.CrossRefPubMed
Metadata
Title
Salvianolic acid B induced upregulation of miR-30a protects cardiac myocytes from ischemia/reperfusion injury
Authors
Dan Li
Jun Wang
Jincai Hou
Jianhua Fu
Jianxun Liu
Ruichao Lin
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1275-x

Other articles of this Issue 1/2016

BMC Complementary Medicine and Therapies 1/2016 Go to the issue

Research article

Aqueous extract of Lithospermi radix attenuates oxaliplatin-induced neurotoxicity in both in vitro and in vivo models

Research article

Methanolic extract of Agerantum conyzoides exhibited toxicity and growth disruption activities against Anopheles gambiae sensu stricto and Anopheles arabiensis larvae

Research article

Anti-tumor effects of Abnormal Savda Munziq on the transplanted cervical cancer (U27) mouse model

Research article

Outcomes from a three-arm randomized controlled trial of frequent immersion in thermoneutral water on cardiovascular risk factors

Research article

Evaluation of Rumex hastatus D. Don for cytotoxic potential against HeLa and NIH/3T3 cell lines: chemical characterization of chloroform fraction and identification of bioactive compounds

Research article

The effects of Nigella sativa on thyroid function, serum Vascular Endothelial Growth Factor (VEGF) – 1, Nesfatin-1 and anthropometric features in patients with Hashimoto’s thyroiditis: a randomized controlled trial