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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Clausenidin induces caspase-dependent apoptosis in colon cancer

Authors: Peter M. Waziri, Rasedee Abdullah, Swee Keong Yeap, Abdul Rahman Omar, Nur Kartinee Kassim, Ibrahim Malami, Chee Wun How, Imaobong Christopher Etti, Mary Ladidi Abu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Clausena excavata Burm.f. is a shrub traditionally used to treat cancer patients in Asia. The main bioactive chemical components of the plant are alkaloids and coumarins. In this study, we isolated clausenidin from the roots of C. excavata to determine its apoptotic effect on the colon cancer (HT-29) cell line.

Method

We examined the effect of clausenidin on cell viability, ROS generation, DNA fragmentation, mitochondrial membrane potential in HT-29 cells. Ultrastructural analysis was conducted for morphological evidence of apoptosis in the treated HT-29 cells. In addition, we also evaluated the effect of clausenidin treatment on the expression of caspase 3 and 9 genes and proteins in HT-29 cells.

Result

Clausenidin induced a G0/G1 cell cycle arrest in HT-29 cells with significant (p < 0.05) dose-dependent increase in apoptotic cell population. The DNA fragmentation assay also showed apoptotic features in the clausenidin-treated HT-29 cells. Clausenidin treatment had caused significant (p < 0.05) increases in the expression of caspase 9 protein and gene in HT-29 cells and mitochondrial ROS and mitochondrial membrane depolarization. The results suggest the involvement of the mitochondria in the caspase-dependent apoptosis in clausenidin-treated colon cancer cells.

Conclusion

Clausenidin induces a caspase-dependent apoptosis in colon cancers through the stimulation of the mitochondria. The study demonstrates the potential of clausenidin for use in the treatment of colon cancers.
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Metadata
Title
Clausenidin induces caspase-dependent apoptosis in colon cancer
Authors
Peter M. Waziri
Rasedee Abdullah
Swee Keong Yeap
Abdul Rahman Omar
Nur Kartinee Kassim
Ibrahim Malami
Chee Wun How
Imaobong Christopher Etti
Mary Ladidi Abu
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1247-1

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