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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

KOTMIN13, a Korean herbal medicine alleviates allergic inflammation in vivo and in vitro

Authors: Eujin Lee, Sun-Gun Kim, Na-Young Park, Hyo-Hyun Park, Kyu-Tae Jeong, Jongkeun Choi, In-Hae Lee, Hwadong Lee, Keuk-Jun Kim, Eunkyung Lee

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

The ethanol extract of KOTMIN13, composed of Inula japonica Flowers, Trichosanthes kirilowii Semen, Peucedanum praeruptorum Radix, and Allium macrostemon Bulbs, was investigated for its anti-asthmatic and anti-allergic activities.

Methods

The anti-asthmatic effects of KOTMIN13 were evaluated on ovalbumin (OVA)-induced murine asthma model. Anti-allergic properties of KOTMIN13 in bone-marrow derived mast cells (BMMC) and passive cutaneous anaphylaxis (PCA) in vivo were also examined.

Results

In asthma model, KOTMIN13 effectively suppressed airway hyperresponsiveness induced by aerosolized methacholine when compared to the levels of OVA-induced mice. KOTMIN13 treatment reduced the total leukocytes, eosinophil percentage, and Th2 cytokines in the bronchoalveolar lavage fluids in OVA-induced mice. The increased levels of eotaxin and Th2 cytokines in the lung as well as serum IgE were decreased by KOTMIN13. The histological analysis shows that the increased inflammatory cell infiltration and mucus secretion were also reduced. In addition, the degranulation and leukotriene C4 production were inhibited in BMMC with IC50 values of 3.9 μg/ml and 1.7 μg/ml, respectively. Furthermore, KOTMIN13 treatment attenuated mast-mediated PCA reaction.

Conclusions

These results demonstrate that KOTMIN13 has anti-asthmatic and anti-allergic effects in vivo and in vitro models.
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Metadata
Title
KOTMIN13, a Korean herbal medicine alleviates allergic inflammation in vivo and in vitro
Authors
Eujin Lee
Sun-Gun Kim
Na-Young Park
Hyo-Hyun Park
Kyu-Tae Jeong
Jongkeun Choi
In-Hae Lee
Hwadong Lee
Keuk-Jun Kim
Eunkyung Lee
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1155-4

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