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BMC Complementary Medicine and Therapies

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Open Access 01-12-2016 | Research article

Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils

Authors: Hanane Boukemara, Margarita Hurtado-Nedelec, Viviana Marzaioli, Dalila Bendjeddou, Jamel El Benna, Jean-Claude Marie

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Abstract

Background

Anvillea garcinii Coss. & Durieu (Anv) plant is used as a traditional North African medicine against several diseases associated with inflammation. At inflammatory sites, reactive oxygen species (ROS) produced in excess by activated phagocyte NADPH oxidase (NOX2) can accentuate inflammatory responses. Thus, we investigated if Anv-water soluble polysaccharides could modulate primary human neutrophil oxidative burst in vitro.

Methods

Human neutrophils were isolated from fresh whole blood and O₂^.- generation was measured by cytochrome c reduction assays. Western blots were used to analyse the translocation of PKC, p47^phox (a key component of NOX2 activity) to neutrophil plasma membrane. Also, myeloperoxidase (MPO) release in the extracellular medium was studied by western blots. Flow cytometric analysis was used to detect CD11b membrane expression.

Results

Water soluble polysaccharides from Anv dose-dependently inhibited N-formyl-methionyl-leucyl-phenylalanine (fMLF)- and phorbol myristate acetate (PMA)-induced O₂^.- generation by human neutrophils. Moreover, Anv-polysaccharides strongly inhibited PMA-induced PKCβ and p47^phox translocation to membranes and p47^phox phosphorylation on Ser328, a main PKC target. In contrast, polysaccharides extract from Zygophyllum gaetulum plant, which is also used as a traditional North African medicine against inflammatory diseases, was ineffective on this PKCβ-p47phox pathway. Further, Anv inhibited important neutrophil degranulation markers corresponding to myeloperoxidase (MPO) release and CD11b membrane expression.

Conclusion

The process of down-regulating NADPH oxidase by polysaccharides extracts from Anv provides new insights into the mechanism of Anv’s anti-inflammatory actions.

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Title: Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils
Authors: Hanane Boukemara
Margarita Hurtado-Nedelec
Viviana Marzaioli
Dalila Bendjeddou
Jamel El Benna
Jean-Claude Marie
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1411-7

At a glance: The STEP trials

Springer Medicine

Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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