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BMC Complementary Medicine and Therapies

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Open Access 01-12-2016 | Research article

Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma

Authors: Gabriela Bueno Franco-Salla, Janesly Prates, Laila Toniol Cardin, Anemari Ramos Dinarte dos Santos, Wilson Araújo da Silva Jr, Bianca Rodrigues da Cunha, Eloiza Helena Tajara, Sonia Maria Oliani, Flávia Cristina Rodrigues-Lisoni

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Abstract

Background

Some plants had been used in the treatment of cancer and one of these has attracted scientific interest, the Euphorbia tirucalli (E. tirucalli), used in the treatment of asthma, ulcers, warts has active components with activities scientifically proven as antimutagenic, anti-inflammatory and anticancer.

Methods

We evaluate the influence of the antitumoral fraction of the E. tirucalli latex in the larynx squamous cell carcinoma (Hep-2), on the morphology, cell proliferation and gene expression. The Hep-2 cells were cultivated in complete medium (MEM 10 %) and treated with E. tirucalli latex for 1, 3, 5 and 7 days. After statistically analyzing the proliferation of the tested cells, the cells were cultivated again for RNA extraction and the Rapid Subtractive Hybridization (RaSH) technique was used to identify genes with altered expression. The genes found using the RaSH technique were analyzed by Gene Ontology (GO) using Ingenuity Systems.

Results

The five genes found to have differential expression were validated by real-time quantitative PCR. Though treatment with E. tirucalli latex did not change the cell morphology in comparison to control samples, but the cell growth was significantly decreased. The RaSH showed change in the expression of some genes, including ANXA1, TCEA1, NGFRAP1, ITPR1 and CD55, which are associated with inflammatory response, transcriptional regulation, apoptosis, calcium ion transport regulation and complement system, respectively. The E. tirucalli latex treatment down-regulated ITPR1 and up-regulated ANXA1 and CD55 genes, and was validated by real-time quantitative PCR.

Conclusions

The data indicate the involvement of E. tirucalli latex in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of larynx cancer.

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Title: Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma
Authors: Gabriela Bueno Franco-Salla
Janesly Prates
Laila Toniol Cardin
Anemari Ramos Dinarte dos Santos
Wilson Araújo da Silva Jr
Bianca Rodrigues da Cunha
Eloiza Helena Tajara
Sonia Maria Oliani
Flávia Cristina Rodrigues-Lisoni
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1115-z

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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