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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Evaluation of anticancer properties of a decoction containing Adenanthera pavonina L. and Thespesia populnea L.

Authors: Indeewari K. S. Lindamulage, Preethi Soysa

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

A decoction composed of Adenanthera pavonina L. and Thespesia populnea L. is currently being used in the treatment of cancer patients.

Methods

Lactate Dehydrogenase (LDH) release, (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT, and Sulforhodamine B (SRB) assays were carried out to study cytotoxicity and anti-proliferative activity against the HEp-2 cells, 24 h post-treatment with the decoction.

Results

The mean (± SD) values of EC50 were 195.50 (±40.68), 120.02 (±29.82) and 77.06 (±8.80) μg/ml for LDH, MTT, and SRB assays respectively. These results strongly correlate the morphological changes observed in cells treated with the decoction. Induction of apoptosis was visualized by fluorescence microscopy stained with ethidium bromide/acridine orange dye mix. In addition, brine shrimp lethality assay showed an EC50 value at a higher concentration (1.96 mg/mL).

Conclusions

These results suggest that the decoction prepared with Adenanthera pavonina L. and Thespesia populnea L. exhibits anti-proliferative activity and induces apoptosis on the HEp-2 cancer cells but no toxicity against Artemia salina.
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Metadata
Title
Evaluation of anticancer properties of a decoction containing Adenanthera pavonina L. and Thespesia populnea L.
Authors
Indeewari K. S. Lindamulage
Preethi Soysa
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1053-9

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