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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity

Authors: Chamira Dilanka Fernando, Preethi Soysa

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Production of reactive oxygen species is a common cause in alcohol induced liver diseases. Decoction prepared from the whole plant of Eriocaulon quinquingulare is prescribed to treat liver disorders. The aim of this study was to investigate the hepatoprotective activity and antioxidant capacity of the water extract of E. quinquangulare in vitro.

Method

The aqueous extract of the whole plant of E. quinquangulare (AEQ) was investigated for its phytochemical constituents, antioxidant and membrane stabilization properties in-vitro. The antioxidant activities of AEQ were investigated using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, nitric oxide scavenging and ferric reducing antioxidant power (FRAP) assays. Membrane stabilizing effect of the extract was determined by hypotonic solution induced human erythrocyte hemolytic assay (HEHA). Further, hepatoprotective activity against ethanol induced hepatotoxicity was carried out using porcine liver slices.

Results

The total phenolics and flavonoids were 10.3 ± 1.6 w/w % gallic acid equivalents and 45.6 ± 3.8 w/w % (−)-epigallocatechin gallate equivalents respectively. The values of EC50 for DPPH, hydroxyl radical and nitric oxide scavenging assays were 37.2 ± 1.7 μg/ml, 170.5 ± 6.6 μg/ml and 31.8 ± 2.2 μg/ml respectively. The reducing capability of AEQ was 6.9 ± 0.2 w/w % L-ascorbic acid equivalents in the FRAP assay. For hypotonic solution induced HEHA, the IC50 was 1.79 ± 0.04 mg/ml. A significant decrease (p < 0.05) was observed in ALT, AST and LDH release from the liver slices treated with AEQ compared to the ethanol treated liver slices. A significant reduction in lipid peroxidation (p < 0.05) was also observed in liver slices treated with the plant extract compared to that of the ethanol treated liver slices.

Conclusions

The results suggest AEQ possess hepatoprotective activity against ethanol induced liver toxicity of porcine liver slices which can be attributed to antioxidant properties and membrane stabilizing effects caused by the plant material.
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Literature
1.
Meera R, Devi P, Kameswari B, Madhumitha B, Merlin NJ. Antioxidant and hepatoprotective activites of Ocimum basilicum Linn. and Trigonella foenum-graecum Linn. against H2O2 and CCl4 induced hepatotoxicity in goat liver. Indian J Exp Biol. 2009;47:584–90.PubMed
2.
3.
Hassan HMM. Hepatoprotective effect of red grape seed extracts against ethanol-induced cytotoxicity. Global J Biotechnol Biochem. 2012;7(2):30–7.
4.
5.
Srivastava A, Shivanandappa T. Hepatoprotective effect of the aqueous extract of the roots of Decalepis hamiltonii against ethanol induced oxidative stress in rats. Hepatol Res. 2006;35(4):267–75.CrossRefPubMed
6.
Momin A. Role of indigenous medicine in primary health care. New Delhi, India: 1st International Seminar on Unani Medicine; 1987. p. 54.
7.
Dassanayake MD, Clayton WD. A Revised Handbook to the Flora of Ceylon. Vol. 11. Rotterdam: A. A. Blakema; 1997. p. 383–6.
8.
Ediriweera ERHSS. A Review on medicinal uses of weeds in Sri Lanka. Trop Agric Res Ext. 2007;10.
9.
Perera MGAN, Soysa SSSBDP, Abeytunga DTU, Ramesh R. Antioxidant and cytotoxic properties of three traditional decoctions used for the treatment of cancer in Sri Lanka. Pharmacogn Mag. 2008;4(15):172–81.
10.
Ugochukwu SC, Uche A, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian J Plant Sci Res. 2013;3(3):10–3.
11.
Ghasemzadeh A, Jaafar HZ, Rahmat A. Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia. BMC Complement Altern Med. 2015;15(1):422.PubMedCentralCrossRefPubMed
12.
Halliwell B, Gutteridge JM, Arnoma OL. The deoxyribose method: a simple test tube assay for the determination of rate constant for reaction of hydroxyl radical. Anal Biochem. 1987;165:215.CrossRefPubMed
13.
Garret D. The quantitative analysis of drugs. Champman and Hall, Japan. 1964;3:456–8.
14.
Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr. 1986;44:307–15.CrossRef
15.
Fernando CD, Soysa P. Total phenolic, flavonoid contents, in-vitro antioxidant activities and hepatoprotective effect of aqueous leaf extract of Atalantia ceylanica. BMC Complement Altern Med. 2014;14:395.PubMedCentralCrossRefPubMed
16.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265.PubMed
17.
Lynch SM, Frei B. Mechanisms of copper- and iron dependent oxidative modification of human low-density lipoprotein. J Lipid Res. 1993;34:1745–51.PubMed
18.
Sadique J, Al-Rqobahs WA, Bughaith, EI-Gindi AR. The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia. 1989;60:525–32.
19.
Al-Sayed E, Martiskainen O, SH S e-D, Sabra ANA, Hammam OA, El-Lakkany NM, et al. Hepatoprotective and Antioxidant Effect of Bauhinia hookeri Extract against Carbon Tetrachloride-Induced Hepatotoxicity in Mice and Characterization of Its Bioactive Compounds by HPLC-PDA-ESI-MS/MS. BioMed Res Int. 2014;2014:1–9.CrossRef
20.
Ho YL, Huang SS, Deng JS, Lin YH, Chang YS, Huang GJ. In vitro antioxidant properties and total phenolic contents of wetland medicinal plants in Taiwan. Bot Stud. 2012;53:55–66.
21.
Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: a review. Trop J Pharm Res. 2008;7:1089–99.CrossRef
22.
Weidinger A, Kozlov AV. Biological activities of reactive oxygen and nitrogen species: oxidative stress versus signal transduction. Biomolecules. 2015;5:472–84.PubMedCentralCrossRefPubMed
23.
Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity Songklanakarin. J Sci Technol. 2004;26(2):211–9.
24.
Harput US, Genç Y, Khan N, Saracoglu I. Radical scavenging effects of different Veronica Species. Rec Nat Prod. 2011;5(2):100–7.
25.
Jagetia GC, Rao SK, Baliga MS, Babu KS. The evaluation of nitric oxide scavenging activity of certain herbal formulations in vitro: a preliminary study. Phytother Res. 2004;18:561–5.CrossRefPubMed
26.
Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem. 1995;43:27–32.CrossRef
27.
Grosse-Siestrup C, Pfeffer J, Unger V, Nagel S, Witt C, Fischer A, Groneberg DA. Isolated hemoperfused slaughterhouse livers as a valid model to study hepatotoxicity. Toxicol Pathol. 2002;30:749.CrossRefPubMed
28.
Naik RS, Mujumdar AM, Ghaskadbi S. Protection of liver cells from ethanol cytotoxicity by curcumin in liver slice culture in vitro. J Ethnopharmacol. 2004;95:31–7.CrossRefPubMed
29.
Mahnaaz M, Swati K, Devasagayam TP, Saroj G. Pterocarpus marsupium extract reveals strong in vitro antioxidant activity. Drug Discov Ther. 2009;3:151–61.
30.
Vilei MT, Granato A, Ferraresso C, Neri D, Carraro P, Gerunda G, Muraca M. Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices. Int J Artif Organs. 2001;24(6):392–6.PubMed
31.
Saraswat B, Visen PK, Patnaik GK, Dhawan BN. Anticholestic effect of picroliv, active hepatoprotective principle of Picrorhiza kurroa against carbon tetrachloride induced cholestatis. Indian J Exp Biol. 1993;31:316–8.PubMed
32.
Christina AJ, Saraswathy GR, Robert SJ, Kothai R, Chidambaranathan N, Nalini G, et al. Inhibition of CCl4-induced liver fibrosis by Piper longum Linn. Phytomedicine. 2006;13:196–8.CrossRefPubMed
33.
Pal R, Nath R, Gill KD. Lipid peroxidation and antioxidant defense enzymes in various regions of adult rat brain after coexposure to cadmium and ethanol. Pharmacol Toxicol. 1993;73:209–14.CrossRefPubMed
34.
Nazari A, Fanaei H, Dehpour AR, Hassanzadeh G, Jafari M, Salehi M, Mohammadi M. Chemical composition and hepatoprotective activity of ethanolic root extract of Taraxacum Syriacum Boiss against acetaminophen intoxication in rats. Bratisl Lek Listy. 2015;116(1):41–6.PubMed
35.
Habibi E, Shokrzadeh M, Chabra A, Naghshvar F, Keshavarz-Maleki R, Ahmadi A. Protective effects of Origanum vulgare ethanol extract against cyclophosphamide-induced liver toxicity in mice. Pharm Biol. 2014;53(1):10–5.CrossRefPubMed
36.
Shameela S, Shamshad S, Indira Priyadarsini A, Lakshmi Devi K. Evaluation of Boerhaavia diffusa for hepatoprotective activity in experimental Wistar rats. Int J Pharm Biol Sci. 2015;5(1):115–22.
Metadata
Title
Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity
Authors
Chamira Dilanka Fernando
Preethi Soysa
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1044-x

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