Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Oral Health
Published in: BMC Oral Health 1/2020

Open Access 01-12-2020 | Biomarkers | Research article

Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co, Ni and Cr) exposed dental technicians: a cross-sectional study

Authors: Titiek Berniyanti, Retno Palupi, Indah L. Kriswandini, Taufan Bramantoro, Indira L. Putri

Published in: BMC Oral Health | Issue 1/2020

Login to get access

Abstract

Background

High concentrations of Co, Ni, and Cr in the blood serum of dental technicians are strongly associated with free radical formation. It has highly reactive properties that can cause further oxidation of molecule in the vicinity.

Purpose

This study intended to investigate whether the Dental Technician occupational exposure of Co, Ni and Cr, could contribute to the high incidence of cancer.

Methods

This was a cross-sectional study to dental technicians, performed after acccepting ethical clearance. Blood was sampled in 3 examinations for Co, Ni, Cr using Atomic Absorbance Spectrophotometry (AAS), MDA was examined with TBARS test, also 8 OHdG and wildtype p53 proteins determined by ELISA method.

Results

Comparative statistical analysis, showing a significant difference (p < 0.05) between levels of Co, Ni, and Cr in exposed groups to the control group. But, not all variables was proven to be positively correlated, only with Cr, and Co, and negatively correlated with wild-type p53.

Conclusion

MDA,8-OHdG and wildtype p53 can be used as genotoxic biomarkers in the metal exposed group, since they can accurately reflect the degree of Oxidative damage.
Appendix
Available only for authorised users
Literature
1.
Kim M-J, Choi Y-J, Kim S-K, Heo S-J, Koak J-Y, Weber FE. Marginal accuracy and internal fit of 3-D printing laser-sintered co-Cr alloy copings. Mater. 2017;10:93.CrossRef
2.
Magaye R, Zhao J, Bowman L, Ding M. Genotoxicity and carcinogenicity of cobalt-, nickel- and copper-based nanoparticles (review). Exp Ther Med. 2012;4:551–61.CrossRef
3.
Hariyani N, Berniyanti T, Setyowati D. Effects of occupational environmental controls on the level of co, Ni and Cr among dental technicians. Int J Environ Sci Dev. 2015;6:643–7.CrossRef
4.
Toyokuni S. Novel aspects of oxidative stress-associated carcinogenesis. Antioxid Redox Signal. 2006;8:1611–208.CrossRef
5.
6.
Valko M, Morris H, Cronin MTD. Metals, toxicity and oxidative stress. Curr Med Chem. 2005;12:1161–208.CrossRef
7.
8.
9.
10.
Elmore SA, Dixon D, Hailey JR, Harada T, Herbert RA, Maronpot RR, et al. Recommendations from the INHAND apoptosis/necrosis working group. Toxicol Pathol. 2016;44:173–88.CrossRef
11.
Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: a review. Eur J Med Chem. 2015;97:55–74.CrossRef
12.
Rohmaniar PD, Berniyanti T, Rahayu RP. The correlation between the use of personal protective equipment and level wild-type p53 of dental technicians in Surabaya. Dent J (Majalah Kedokt Gigi). 2017;50:19–22.CrossRef
13.
Scharf B, Clement CC, Zolla V, Perino G, Yan B, Elci SG, et al. Molecular analysis of chromium and cobalt-related toxicity. Sci Rep. 2014;4:5729.CrossRef
14.
Sorahan T. Mortality of workers at a plant manufacturing nickel alloys, 1958-2000. Occup Med (Chic Ill). 2004;54:28–34.CrossRef
15.
Goullé J-P, Mahieu L, Castermant J, Neveu N, Bonneau L, Lainé G, et al. Metal and metalloid multi-elementary ICP-MS validation in whole blood, plasma, urine and hair: reference values. Forensic Sci Int. 2005;153:39–44.CrossRef
16.
Kehrer JP. The Haber-Weiss reaction and mechanisms of toxicity. Toxicology. 2000;149:43–50.CrossRef
17.
Galaris D, Evangelou A. The role of oxidative stress in mechanisms of metal-induced carcinogenesis. Crit Rev Oncol Hematol. 2002;42:93–103.CrossRef
18.
Jensen SJK. Oxidative stress and free radicals. J Mol Struct Theochem. 2003;666:387–92.CrossRef
19.
Abeti R, Parkinson MH, Hargreaves IP, Angelova PR, Sandi C, Pook MA, et al. Mitochondrial energy imbalance and lipid peroxidation cause cell death in Friedreich’s ataxia. Cell Death Dis. 2016;7:–e2237.CrossRef
20.
Najeeb Q, Bhaskar N, Masood I, Wadhwa S, Kaur H, Ishaq S. Malondialdehyde (MDA) superoxide dismutase (SOD) levels - distinguishing parameters betweenbenign malignant pleural effusions. Free Radicals Antioxidants. 2012;2(2):8–11.CrossRef
21.
Nielsen F, Mikkelsen BB, Nielsen B, Andersen R, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem. 1997;43:1209–14.CrossRef
22.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Mini-review free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006;160:1–40.CrossRef
23.
Valavanidis A, Vlahoyianni T, Fiotakis K. Comparative study of the formation of oxidative damage marker 8-hydroxy-2’-deoxyguanosine (8-OHdG) adduct from the nucleoside 2’-deoxyguanosine by transition metals and suspensions of particulate matter in relation to metal content and redox reactivity. Free Radic Res. 2005;39:1071–81.CrossRef
24.
Hartwig A. Metal interaction with redox regulation: an integrating concept in metal carcinogenesis? Free Radic Biol Med. 2013;55:63–72.CrossRef
25.
Norbury CJ, Zhivotovsky B. DNA damage-induced apoptosis. Oncogene. 2004;23:2797–808.CrossRef
26.
Ljungman M. Dial 9-1-1 for p53: mechanisms of p53 activation by cellular stress. Neoplasia. 2000;2:208–25.CrossRef
27.
Willis A, Jung EJ, Wakefield T, Chen X. Mutant p53 exerts a dominant negative effect by preventing wild-type p53 from binding to the promoter of its target genes. Oncogene. 2004;23:2330–8.CrossRef
28.
Rivin N, Brosh R, Oren M, Rotter V. Mutations in the p53 tumor suppressor gene: important milestones at the various steps of tumorigenesis. Gene Cancer. 2011;2:466–74.CrossRef
29.
Wong R-H, Kuo C-Y, Hsu M-L, Wang T-Y, Chang P-I, Wu T-H, et al. Increased levels of 8-Hydroxy-2′-Deoxyguanosine attributable to carcinogenic metal exposure among schoolchildren. Environ Health Perspect. 2005;113:1386–90.CrossRef
30.
Koedrith P, Seo YR. Advances in carcinogenic metal toxicity and potential molecular markers. Int J Mol Sci. 2011;12:9576–95.CrossRef
31.
Matsui A, Ikeda T, Enomoto K, Hosoda K, Nakashima H, Omae K, et al. Increased formation of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, in human breast cancer tissue and its relationship to GSTP1 and COMT genotypes. Cancer Lett. 2000;151:87–95.CrossRef
32.
Perra MT, Maxia C, Corbu A, Minerba L, Demurtas P, Colombari R, et al. Oxidative stress in pterygium: relationship between p53 and 8-hydroxydeoxyguanosine. Mol Vis. 2006;12:1136–42.PubMed
Metadata
Title
Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co, Ni and Cr) exposed dental technicians: a cross-sectional study
Authors
Titiek Berniyanti
Retno Palupi
Indah L. Kriswandini
Taufan Bramantoro
Indira L. Putri
Publication date
01-12-2020
Publisher
BioMed Central
Keyword
Biomarkers
Published in
BMC Oral Health / Issue 1/2020
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-020-1049-1

Other articles of this Issue 1/2020

BMC Oral Health 1/2020 Go to the issue

Research article

Dental implant location via surface scanner: a pilot study

Research article

Do probiotics promote oral health during orthodontic treatment with fixed appliances? A systematic review

Research article

An in vitro study of the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma

Research article

Spectrophotometric analysis evaluating apical microleakage in retrograde filling using GIC, MTA and biodentine: an in-vitro study

Research article

Oral health problems among palliative and terminally ill patients: an integrated systematic review

Research article

In vitro comparison of cyclic fatigue resistance of TruNatomy in single and double curvature canals compared with different nickel-titanium rotary instruments