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Open Access 01-12-2020 | Candida Balanitis | Research article

Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces

Authors: Loyse Martorano-Fernandes, Nadiny Cezar Rodrigues, Maria Heloísa de Souza Borges, Yuri Wanderley Cavalcanti, Leopoldina de Fátima Dantas de Almeida

Published in: BMC Oral Health | Issue 1/2020

Abstract

Background

In oral candidiasis models, Candida albicans and Streptococcus salivarius sp. biofilms have an antagonistic relationship. Due to this, S. salivarius have been used experimentally as probiotic. However, the interaction between these microorganisms in the peri-implantitis-like microenvironment remains unknown. This study aimed to evaluate the interaction between C. albicans and S. salivarius biofilms developed on titanium surfaces, under reduced oxygen levels.

Methods

Titanium specimens were pre-conditioned with artificial saliva (1 h, 37 °C). Single-species biofilms of C. albicans (ATCC 90028) and co-culture biofilms of C. albicans and S. salivarius (ATCC 7073) was developed for 24 and 72 h on titanium specimens. Subsequently, the effect of these intervals of biofilm formation and the interactions among the cells were evaluated. Biofilms from cultures were collected and analyzed for cell viability (CFU/mL), biofilm biomass, and total protein dosage. Data were analyzed using Mann–Whitney test (α = 5%). In addition, co-culture biofilms were analyzed using fluorescence microscopy.

Results

C. albicans growth did not change due to the presence of S. salivarius. Besides, co-culture biofilms showed a significant difference in the number of viable cells between 24 and 72 h of biofilm development (p < 0.05). The highest biofilm biomass and protein dosage were observed in co-cultures at 72 h of biofilm development. Fluorescence microscopy showed that co-cultures biofilms at 24 h have limited number of pseudo-hyphal and hyphae cells of C. albicans. At 72 h, these types of cells have increased. S. salivarius in both stages of development was present in some clusters surrounded by C. albicans.

Conclusions

Co-cultivation of C. albicans with S. salivarius in biofilms developed on titanium surfaces, under lower oxygen levels, did not affect fungus growth. In addition, S. salivarius did not hind C. albicans virulence. These findings suggest that the use of S. salivarius as a probiotic would be ineffective in peri-implant disease treatment.

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Title: Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces
Authors: Loyse Martorano-Fernandes
Nadiny Cezar Rodrigues
Maria Heloísa de Souza Borges
Yuri Wanderley Cavalcanti
Leopoldina de Fátima Dantas de Almeida
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Candida Balanitis
Candidiasis
Candida Albicans
Candidiasis
Streptococci
Published in: BMC Oral Health / Issue 1/2020
Electronic ISSN: 1472-6831
DOI: https://doi.org/10.1186/s12903-020-01334-w

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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