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International Journal of Implant Dentistry

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Open Access 01-12-2015 | Research

Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites

Authors: Frank Schwarz, Kathrin Becker, Sebastian Rahn, Andrea Hegewald, Klaus Pfeffer, Birgit Henrich

Published in: International Journal of Implant Dentistry | Issue 1/2015

Abstract

Background

The potential role of fungal organisms and their co-aggregation with either periodontopathogens or opportunistic pathogens at peri-implantitis sites is unknown. The aim of the present study was to qualitatively/quantitatively analyze and correlate fungal organisms and bacterial species at peri-implantitis sites.

Methods

In a total of 29 patients, submucosal/subgingival plaque samples were collected at peri-implantitis and healthy implant sites as well as teeth with a history of periodontitis (controls). A real-time PCR assay was established for the qualification of fungal organisms and a TaqMan assay for the quantification of Porphyromonas gingivalis, Parvimonas micra, Tannerella forsythia, Mycoplasma salivarium, Veillonella parvula, and Staphylococcus aureus.

Results

Fungal organisms were more frequently identified at peri-implantitis (31.6%) (i.e., Candida albicans, Candida boidinii, Penicillium spp., Rhodotorula laryngis, Paelicomyces spp., Saccharomycetes, Cladosporium cladosporioides) and healthy implant sites (40% - Candida dubliniensis, C. cladosporioides) than at selected teeth (20% - C. albicans, Fusarium solani). At implant sites, fungal organisms were significantly correlated with P. micra and T. forsythia.

Conclusions

Candida spp. and other fungal organisms were frequently identified at peri-implantitis as well as healthy implant sites and co-colonized with P. micra and T. forsythia.

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Title: Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
Authors: Frank Schwarz
Kathrin Becker
Sebastian Rahn
Andrea Hegewald
Klaus Pfeffer
Birgit Henrich
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Implant Dentistry / Issue 1/2015
Electronic ISSN: 2198-4034
DOI: https://doi.org/10.1186/s40729-015-0010-6

At a glance: The STEP trials

Springer Medicine

Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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