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BMC Oral Health
Published in: BMC Oral Health 1/2020

Open Access 01-12-2020 | Research article

Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro

Authors: Yalda Khosravi, Rala D.P. Kandukuri, Sara R. Palmer, Erin S. Gloag, Sergey M. Borisov, E. Michelle Starke, Marilyn T. Ward, Purnima Kumar, Dirk de Beer, Arjun Chennu, Paul Stoodley

Published in: BMC Oral Health | Issue 1/2020

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Abstract

Background

Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents.

Methods

To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human saliva and dental plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device.

Results

HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qPCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions.

Conclusion

We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.
Appendix
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Metadata
Title
Use of an oxygen planar optode to assess the effect of high velocity microsprays on oxygen penetration in a human dental biofilms in-vitro
Authors
Yalda Khosravi
Rala D.P. Kandukuri
Sara R. Palmer
Erin S. Gloag
Sergey M. Borisov
E. Michelle Starke
Marilyn T. Ward
Purnima Kumar
Dirk de Beer
Arjun Chennu
Paul Stoodley
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2020
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-020-01217-0

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