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BMC Oral Health
Published in: BMC Oral Health 1/2015

Open Access 01-12-2015 | Research article

Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case–control study

Published in: BMC Oral Health | Issue 1/2015

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Abstract

Background

Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Sortase is a transpeptidase that anchors several surface proteins to the S. mutans cell wall and has been shown to play a major role in cariogenicity. The purpose of this study was to explore the genetic polymorphisms of the sortase gene (srtA) and the social-behavioural factors associated with dental caries in children with S. mutans.

Methods

In this case–control study, 121 S. mutans strains were separately selected from caries-free children and high-severity caries children for sequencing of the srtA gene. Social and behavioural data were collected by self-administered questionnaires. Genomic DNA was extracted from S. mutans strains and amplified by PCR to obtain the srtA gene. The purified PCR products were sequenced and analysed for mutations with ABI Variant Reporter software. The distribution of missense mutations and the mean of social-behavioural factors were compared between the groups. A multiple logistic regression model was used to control for confounding factors.

Results

The mutation frequencies at loci 168 (P = 0.023) and 470 (P = 0.032) were significantly different between the groups. The best-fitting model showed that greater age, high frequencies of solid sugar consumption, prolonged breastfeeding, a high proportion of visible plaque, and S. mutans with a T at locus 168 of the srtA gene were associated with high-severity caries in children (P < 0.05). Children carrying a G at locus 168 of S. mutans had a decreased risk for high-severity caries (OR = 0.32, 95% CI = 0.12–0.86) compared with those carrying a T.

Conclusions

The present study suggested that the locus 168 missense mutation of the srtA gene may correlate with caries susceptibility in children with S. mutans. In addition, age, duration of breastfeeding, solid sugar consumption, and poor oral hygiene contributed to this complex disease.
Literature
1.
2.
Mulu W, Demilie T, Yimer M, Meshesha K, Abera B. Dental caries and associated factors among primary school children in Bahir Dar city: a cross-sectional study. BMC Res Notes. 2014;7:949.CrossRefPubMedPubMedCentral
3.
Zhou Y, Lin HC, Lo EC, Wong MC. Risk indicators for early childhood caries in 2-year-old children in southern China. Aust Dent J. 2011;56:33–9.CrossRefPubMed
4.
Folayan MO, Chukwumah NM, Onyejaka N, Adeniyi AA, Olatosi OO. Appraisal of the national response to the caries epidemic in children in Nigeria. BMC Oral Health. 2014;14:76.CrossRefPubMedPubMedCentral
5.
Struzycka I, Wierzbicka M, Jodkowska E, Rusyan E, Ganowicz E, Fidecki M. Oral health and prophylactic-therapeutic needs of children aged 6 years in Poland in 2012. Przegl Epidemiol. 2014;68:53–7.PubMed
6.
Qi XQ. Report of the Third National Oral Health Survey in China (In Chinese). Beijing: People’s Medical Publishing House; 2008. p. 60–1.
7.
Krzysciak W, Jurczak A, Koscielniak D, Bystrowska B, Skalniak A. The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis. 2014;33:499–515.CrossRefPubMed
8.
Jenkinson HF, Demuth DR. Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol. 1997;23:183–90.CrossRefPubMed
9.
Lee SF, Boran TL. Roles of sortase in surface expression of the major protein adhesin P1, saliva-induced aggregation and adherence, and cariogenicity of Streptococcus mutans. Infect Immun. 2003;71:676–81.CrossRefPubMedPubMedCentral
10.
Igarashi T, Asaga E, Goto N. The sortase of Streptococcus mutans mediates cell wall anchoring of a surface protein antigen. Oral Microbiol Immunol. 2003;18:266–9.CrossRefPubMed
11.
Lee SF, McGavin MK. Identification of a point mutation resulting in loss of cell wall anchoring activity of SrtA of Streptococcus mutans NG5. Infect Immun. 2004;72:4314–7.CrossRefPubMedPubMedCentral
12.
Napimoga MH, Hofling JF, Klein MI, Kamiya RU, Goncalves RB. Tansmission, diversity and virulence factors of Sreptococcus mutans genotypes. J Oral Sci. 2005;47:59–64.CrossRefPubMed
13.
Zhou Y, Yang JY, Zhi QH, Tao Y, Qiu RM, Lin HC. Factors associated with colonization of Streptococcus mutans in 8 to 32-month-old children: a cohort study. Aust Dent J. 2013;58:507–13.CrossRefPubMed
14.
Su Z. The sample size calculation and statistical analysis of case control study. In: Fang JQ, Sun ZQ, editors. Health Statistics (In Chinese). Beijing: People’s Medical Publishing House; 2008. p. 347.
15.
Zhang XH, Zhou Y, Zhi QH, Tao Y, Lin HC. Genetic polymorphisms of the sortase A gene and early childhood caries in two-year-old children. Arch Oral Biol. 2012;57:948–53.CrossRefPubMed
16.
Zhang R, Lin HC, Zhi QH. Caries prevalence of primary teeth and related factors in urban and rural young children of Guangzhou (In Chinese). Chin J Pract Stomatol. 2008;1:470–2.
17.
Zhang XH, Zhou Y, Lin HC. Acquisition of Streptococcus mutans and cell adherence among 2-year old children with different caries experience (in Chinese). Chin J Pract Stomatol. 2009;2:727–9.
18.
World Health Organization. Oral health survey, Basic Methods. 4th ed. Geneva: World Health Organization; 1997.
19.
FDI Commission on Oral Health, Research and Epidemiology. A review of the developmental defects of enamel index (DDE index). Int Dent J. 1992;42:411–26.
20.
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J. 1975;25:229–35.PubMed
21.
Gold OG, Jordan HV, Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973;18:1357–64.CrossRefPubMed
22.
Shklair IL, Keene HJ. A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Arch Oral Biol. 1974;19:1079–81.CrossRefPubMed
23.
Martins-Junior PA, Vieira-Andrade RG, Correa-Faria P, Oliveira-Ferreira F, Marques LS, Ramos-Jorge ML. Impact of early childhood caries on the oral health-related quality of life of preschool children and their parents. Caries Res. 2013;47:211–8.CrossRefPubMed
24.
Igarashi T. Deletion in sortase gene of Streptococcous mutans Ingbritt. Oral Microbiol Immunol. 2004;19:210–3.CrossRefPubMed
25.
Vachirarojpisan T, Shinada K, Kawaguchi Y, Laungwechakan P, Somkote T, Detsomboonrat P. Early childhood caries in children aged 6–19 months. Community Dent Oral Epidemiol. 2004;32:133–42.CrossRefPubMed
26.
Bowen WH, Lawrence RA. Comparison of the cariogenicity of cola, honey, cow milk, human milk, and sucrose. Pediatrics. 2005;116:921–6.CrossRefPubMed
27.
Moye ZD, Zeng L, Burne RA. Fueling the caries process: carbohydrate metabolism and gene regulation by Streptococcus mutans. J Oral Microbiol. 2014;6:24878.CrossRef
Metadata
Title
Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case–control study
Publication date
01-12-2015
Published in
BMC Oral Health / Issue 1/2015
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-015-0039-1

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