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BMC Dermatology
Published in: BMC Dermatology 1/2017

Open Access 01-12-2017 | Research article

Expression profiling and bioinformatic analyses suggest new target genes and pathways for human hair follicle related microRNAs

Authors: Lara M. Hochfeld, Thomas Anhalt, Céline S. Reinbold, Marisol Herrera-Rivero, Nadine Fricker, Markus M. Nöthen, Stefanie Heilmann-Heimbach

Published in: BMC Dermatology | Issue 1/2017

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Abstract

Background

Human hair follicle (HF) cycling is characterised by the tight orchestration and regulation of signalling cascades. Research shows that micro(mi)RNAs are potent regulators of these pathways. However, knowledge of the expression of miRNAs and their target genes and pathways in the human HF is limited. The objective of this study was to improve understanding of the role of miRNAs and their regulatory interactions in the human HF.

Methods

Expression levels of ten candidate miRNAs with reported functions in hair biology were assessed in HFs from 25 healthy male donors. MiRNA expression levels were correlated with mRNA-expression levels from the same samples. Identified target genes were tested for enrichment in biological pathways and accumulation in protein-protein interaction (PPI) networks.

Results

Expression in the human HF was confirmed for seven of the ten candidate miRNAs, and numerous target genes for miR-24, miR-31, and miR-106a were identified. While the latter include several genes with known functions in hair biology (e.g., ITGB1, SOX9), the majority have not been previously implicated (e.g., PHF1). Target genes were enriched in pathways of interest to hair biology, such as integrin and GnRH signalling, and the respective gene products showed accumulation in PPIs.

Conclusions

Further investigation of miRNA expression in the human HF, and the identification of novel miRNA target genes and pathways via the systematic integration of miRNA and mRNA expression data, may facilitate the delineation of tissue-specific regulatory interactions, and improve our understanding of both normal hair growth and the pathobiology of hair loss disorders.
Appendix
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Metadata
Title
Expression profiling and bioinformatic analyses suggest new target genes and pathways for human hair follicle related microRNAs
Authors
Lara M. Hochfeld
Thomas Anhalt
Céline S. Reinbold
Marisol Herrera-Rivero
Nadine Fricker
Markus M. Nöthen
Stefanie Heilmann-Heimbach
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Dermatology / Issue 1/2017
Electronic ISSN: 1471-5945
DOI
https://doi.org/10.1186/s12895-017-0054-9

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