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BMC Urology

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Open Access 01-12-2017 | Research article

Expression and function of phosphodiesterases (PDEs) in the rat urinary bladder

Authors: Xiaofei Zhu, Kui Zhai, Yue Mi, Guangju Ji

Published in: BMC Urology | Issue 1/2017

Abstract

Background

It has been shown that hosphodiesterases (PDEs) play an important role in mediating the smooth muscle tone of rat urinary bladder. However, the gene expression profiles of them were still unknown.

Methods

Urinary bladder Strips were obtained from both neonatal and adult Sprague-Dawley rats. RT-PCR/western blot and organ bath were used to measure the expression and function of PDEs.

Results

Adult rat urinary bladder expressed various PDE mRNA with the following rank order: PDE5A ≈ PDE9A ≈ PDE10A > PDE2A ≈ PDE4A ≈ PDE4D > PDE4B ≈ PDE3B ≈ PDE8B ≈ PDE7A ≈ PDE7B > PDE1A. PDE1B, PDE1C, PDE3A, PDE4C, PDE8A, and PDE11A were not detected. Of interest, the mRNA and protein of PDE3A were significantly decreased in adult rat urinary bladder compared to neonatal rat urinary bladder. Cilostamide, a specific inhibitor for PDE3, significantly inhibited the amplitude and frequency of carbachol-enhanced phasic contractions of neonatal rat bladder strips by 38.8% and 12.1%, respectively. Compared to the neonatal rat bladder, the effect of cilostamide was significantly blunted in adult rat urinary bladder: the amplitude and frequency of carbachol-enhanced phasic contractions were decreased by 13.4% (P < 0.01 vs neonatal rat bladder) and 4.4%, respectively. However, the mRNA and the protein levels of PDE3B were similar between neonatal and adult rat bladder.

Conclusion

We found that several PDE isoforms were expressed in the rat urinary bladder with distinct levels. Moreover, we showed that the function of PDE3 was blunted in adult rat bladder likely due to the decreased expression of PDE3A.

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Title: Expression and function of phosphodiesterases (PDEs) in the rat urinary bladder
Authors: Xiaofei Zhu
Kui Zhai
Yue Mi
Guangju Ji
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Urology / Issue 1/2017
Electronic ISSN: 1471-2490
DOI: https://doi.org/10.1186/s12894-017-0244-0

ACC 2024 Congress

Springer Medicine

Expression and function of phosphodiesterases (PDEs) in the rat urinary bladder

Abstract

Background

Methods

Results

Conclusion

ACC 2024 Congress

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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