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BMC Urology
Published in: BMC Urology 1/2017

Open Access 01-12-2017 | Research article

Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse

Authors: Kai Li, Xuedong Wei, Guangbo Zhang, Miao Li, Xuefeng Zhang, Chenhao Zhou, Jianquan Hou, Hexing Yuan

Published in: BMC Urology | Issue 1/2017

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Abstract

Background

B7-H3, a member of the B7 family of the Ig superfamily of proteins, has been detected in the epididymis, which is a storage organ related to sperm maturation. However, the characteristics of its expression in different regions of the epididymis remain unknown. Our aim was to investigate the expression of B7-H3 in the caput, corpus, and cauda of the epididymis.

Methods

We extracted epididymis specimens from adult male C57BL/6 mice. The expression of B7-H3 was then measured with immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and western blotting.

Results

B7-H3 protein was predominantly detected on the membrane and in the cytoplasm of the principal cells in the epididymis. Moreover, the level of B7-H3 in the corpus of the mouse epididymis was significantly higher than that in the caput (p < 0.05) or the cauda of the epididymis (P < 0.05). However, there was no remarkable difference in the level of B7-H3 between the caput and the cauda (p > 0.05).

Conclusions

The caput, corpus, and cauda of the mouse epididymis all expressed B7-H3 protein. However, the levels of B7-H3 were different in the three regions of the epididymis.
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Metadata
Title
Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse
Authors
Kai Li
Xuedong Wei
Guangbo Zhang
Miao Li
Xuefeng Zhang
Chenhao Zhou
Jianquan Hou
Hexing Yuan
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Urology / Issue 1/2017
Electronic ISSN: 1471-2490
DOI
https://doi.org/10.1186/s12894-017-0215-5

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