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Open Access 01-12-2015 | Research article

Inverse relationship of Rho kinase and myosin-light chain kinase expression in the aging human detrusor smooth muscle

Authors: Timo Kirschstein, Theresa Sahre, Karoline Kernig, Chris Protzel, Katrin Porath, Rüdiger Köhling, Oliver W. Hakenberg

Published in: BMC Urology | Issue 1/2015

Abstract

Background

Rho kinase (ROCK) and myosin-light chain kinase (MLCK) are key enzymes in smooth muscle contraction. Previous data have suggested that ROCK contribution to human detrusor contraction is increasing with age. Here, we have analyzed the transcriptional expression of Rho kinase isoforms (ROCK1 and ROCK2) as well as MLCK in the aging human detrusor smooth muscle obtained from resected tissue.

Methods

Small pieces of macroscopically healthy human detrusor smooth muscle (urothelium-free) were prepared for quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). Transcript expression (mRNA level) of the target genes ROCK1, ROCK2 and MLCK was normalized to three common reference genes (glyceraldehyde-3-phosphate dehydrogenase, β-actin, phosphoglycerate kinase 1).

Results

We found that across all ages the expression level of ROCK (i.e. ROCK1 and ROCK2 together) was almost equal to that of MLCK in the human bladder. Further, ROCK2 showed a significantly higher expression level than ROCK1. Among all subjects, there was no significant correlation of any single target gene to age, but expression levels of ROCK and MLCK were inversely correlated. Moreover, the within-subject analysis revealed that the ROCK-to-MLCK ratio showed a significantly negative correlation to age. Thus, within a given subject, there is a relative ROCK down-regulation and concomitant MLCK up-regulation.

Conclusions

Together with previous data in human detrusor specimens showing increased ROCK contribution to detrusor contraction, we speculate that the drop of the ROCK-to-MLCK ratio may occur as an attempt to compensate for the increased Rho kinase activity.

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Title: Inverse relationship of Rho kinase and myosin-light chain kinase expression in the aging human detrusor smooth muscle
Authors: Timo Kirschstein
Theresa Sahre
Karoline Kernig
Chris Protzel
Katrin Porath
Rüdiger Köhling
Oliver W. Hakenberg
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Urology / Issue 1/2015
Electronic ISSN: 1471-2490
DOI: https://doi.org/10.1186/s12894-015-0098-2

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Inverse relationship of Rho kinase and myosin-light chain kinase expression in the aging human detrusor smooth muscle

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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