Top

Published in:

01-03-2013 | EDUCATIONAL SERIES – BLUE SERIES

Novel insight into the role of GAPDH playing in tumor

Authors: Chunmei Guo, Shuqing Liu, Ming-Zhong Sun

Published in: Clinical and Translational Oncology | Issue 3/2013

Abstract

The role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) being consistently regarded as the main housekeeping gene and reference gene/protein for expression quantification in tumors has been gradually questioned and challenged by accumulated experiment evidence. The current review notified that the GAPDH expression was deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Interestingly, GAPDH was commonly up-regulated in a variety of types of cancer, which was revealed to be potentially required for the cancer cell growth and tumor formation. The relevant mechanisms were also discussed in current review. This work might provide useful insights for future studies on GAPDH in tumors.

Jiang QH, Liu DG, Zheng RH et al (2002) Comparison of expression levels of S29, 18s rRNA and GAPDH genes in lung tumor tissues. Tumor 22:201–203

Rubporn A, Srisomsap C, Subhasitanont P et al (2009) Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line. Cancer Genom Proteom 6:229–237

Jung M, Ramankulov A, Roigas J et al (2007) In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR. BMC Mol Biol 8:47PubMedCrossRef

Engel M, Seifert M, Theisinger B et al (1998) Glyceraldehyde-3-phosphate dehydrogenase and Nm23-H1/nucleoside diphosphate kinase A. Two old enzymes combine for the novel Nm23 protein phosphotransferase function. J Biol Chem 273:20058–20065PubMedCrossRef

Revillion F, Pawlowski V, Hornez L et al (2000) Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer. Eur J Cancer 36:1038–1042PubMedCrossRef

Matrisian LM, Rautmann G, Magun BE et al (1985) Epidermal growth factor or serum stimulation of rat fibroblasts induces an elevation in mRNA levels for lactate dehydrogenase and other glycolytic enzymes. Nucl Acids Res 13:711–726PubMedCrossRef

Valenti MT, Bertoldo F, Dalle Carbonare L et al (2006) The effect of bisphosphonates on gene expression: GAPDH as a housekeeping or a new target gene? BMC Cancer 6:49–55PubMedCrossRef

Honda K, Yamada T, Hayashida Y et al (2005) Actinin-4 increases cell motility and promotes lymph node metastasis of colorectal cancer. Gastroenterology 128:51–62PubMedCrossRef

Khimani AH, Mhashilkar AM, Mikulskis A et al (2005) Housekeeping genes in cancer: normalization of array data. Biotechniques 38:739–745PubMedCrossRef

10.

Kim JW, Kim TE, Kim YK et al (1999) Antisense oligodeoxynucleotide of glyceraldehyde-3-phosphate dehydrogenase gene inhibits cell proliferation and induces apoptosis in human cervical carcinoma cell lines. Antisense Nucl Acid Drug Dev 9:507–513CrossRef

11.

Sirover MA (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta 1432:159–184PubMedCrossRef

12.

Tokunaga K, Nakamura Y, Sakata K et al (1987) Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers. Cancer Res 47:5616–5619PubMed

13.

Cuezva JM, Chen G, Alonso AM et al (2004) The bioenergetic signature of lung adenocarcinomas is a molecular marker of cancer diagnosis and prognosis. Carcinogenesis 25:1157–1163PubMedCrossRef

14.

Demarse NA, Ponnusamy S, Spicer EK et al (2009) Direct binding of glyceraldehyde-3-phosphate dehydrogenase to telomeric DNA protects telomeres against chemotherapy-induced rapid degradation. J Mol Biol 394:789–803PubMedCrossRef

15.

Vila MR, Nicolas A, Morote J et al (2000) Increased glyceraldehyde-3-phosphate dehydrogenase expression in renal cell carcinoma identified by RNA-based, arbitrarily primed polymerase chain reaction. Cancer 89:152–164PubMedCrossRef

16.

Epner DE, Partin AW, Schalken JA et al (1993) Association of glyceraldehyde-3-phosphate dehydrogenase expression with cell motility and metastatic potential of rat prostatic adenocarcinoma. Cancer Res 53:1995–1997PubMed

17.

Alexander MC, Lomanto M, Nasrin N et al (1988) Insulin stimulates glyceraldehyde-3-phosphate dehydrogenase gene expression through cis-acting DNA sequences. Proc Natl Acad Sci USA 85:5092–5096PubMedCrossRef

18.

Blanquicett C, Johnson MR, Heslin M et al (2002) Housekeeping gene variability in normal and carcinomatous colorectal and liver tissues: applications in pharmacogenomic gene expression studies. Anal Biochem 303:209–214PubMedCrossRef

19.

Suzuki A, Iizuka A, Komiyama M et al (2010) Identification of melanoma antigens using a Serological Proteome Approach (SERPA). Cancer Genom Proteom 7:17–23

20.

Ohl F, Jung M, Radonic A et al (2006) Identification and validation of suitable endogenous reference genes for gene expression studies of human bladder cancer. J Urol 175:1915–1920PubMedCrossRef

21.

Wang XY, Yang XY, Yang HY et al (2005) Study on the correlations of glyceraldehydes-3-phosphate dehydrogenase with human glioma. Clin J Contemp Neurol Neurosurg 5:35–39

22.

Nguewa PA, Agorreta J, Blanco D et al (2008) Identification of importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens. BMC Mol Biol 9:103PubMedCrossRef

23.

Parkin DM, Bray F, Ferlay J et al (2002) Global cancer statistics. CA Cancer J Clin 55:74–108CrossRef

24.

Schmidt B, Engel E, Carstensen T et al (2005) Quantification of free RNA in serum and bronchial lavage: a new diagnostic tool in lung cancer detection? Lung Cancer 48:145–147PubMedCrossRef

25.

Lopez-Beltran A, Scarpelli M, Montironi R et al (2006) 2004 WHO classification of the renal tumors of the adults. Eur Urol 49:798–805PubMedCrossRef

26.

Diamond I, Legg A, Schneider JA et al (1978) Glycolysis in quiescent cultures of 3T3 cells. Stimulation by serum, epidermal growth factor, and insulin in intact cells and persistence of the stimulation after cell homogenization. J Biol Chem 253:866–871PubMed

27.

Llovet JM, Burroughs A, Bruix J (2003) Hepatocellular carcinoma. Lancet 362:1907–1917PubMedCrossRef

28.

Rubie C, Kempf K, Hans J et al (2005) Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues. Mol Cell Probes 19:101–109PubMedCrossRef

29.

Waxman S, Wurmbach E (2007) De-regulation of common housekeeping genes in hepatocellular carcinoma. BMC Genom 8:243–251CrossRef

30.

Gong Y, Cui L, Minuk GY (1996) Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28s-ribosomal RNA gene expression in human hepatocellular carcinoma. Hepatology 23:734–737PubMedCrossRef

31.

Schek N, Hall BL, Finn OJ (1988) Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in human pancreatic adenocarcinoma. Cancer Res 48:6354–6359PubMed

32.

Chang YT, Tseng HC, Huang CC et al (2011) Relative down-regulation of apoptosis and autophagy genes in colorectal cancer. Eur J Clin Invest 41:84–92PubMedCrossRef

33.

Dydensborg AB, Herring E, Auclair J et al (2006) Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon. Am J Physiol Gastrointest Liver Physiol 290:G1067–G1074PubMedCrossRef

34.

Goidin D, Mamessier A, Staquet MJ et al (2001) Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations. Anal Biochem 295:17–21PubMedCrossRef

35.

Ripple MO, Wilding G (1995) Alteration of glyceraldehyde-3-phosphate dehydrogenase activity and messenger RNA content by androgen in human prostate carcinoma cells. Cancer Res 55:4234–4236PubMed

36.

Persons DA, Schek N, Hall BL et al (1989) Increased expression of glycolysis-associated genes in oncogene-transformed and growth-accelerated states. Mol Carcinog 2:88–94PubMedCrossRef

37.

Aithal HN, Toback FG, Cryst C (1980) Enhancement of renal medullary pyruvate kinase activity during cell proliferation induced by potassium depletion. Am J Physiol 238:E377–E383PubMed

38.

Cool BL, Sirover MA (1989) Immunocytochemical localization of the base excision repair enzyme uracil DNA glycosylase in quiescent and proliferating normal human cells. Cancer Res 49:3029–3036PubMed

39.

Mansur NR, Meyer-Siegler K, Wurzer JC et al (1993) Cell cycle regulation of the glyceraldehyde-3-phosphate dehydrogenase/uracil DNA glycosylase gene in normal human cells. Nucl Acids Res 21:993–998PubMedCrossRef

Title: Novel insight into the role of GAPDH playing in tumor
Authors: Chunmei Guo
Shuqing Liu
Ming-Zhong Sun
Publication date: 01-03-2013
Publisher: Springer Milan
Published in: Clinical and Translational Oncology / Issue 3/2013
Print ISSN: 1699-048X
Electronic ISSN: 1699-3055
DOI: https://doi.org/10.1007/s12094-012-0924-x

Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras

Prof. Fabrice Barlesi

Developed by: Springer Medicine

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2013

IMRT: preliminary results in a series of advanced head-and-neck cancer patients

Practice patterns in the management of prostate cancer in Spain: results from a national survey among radiation oncologists in 2009

Development and characterization of an isogenic cell line with a radioresistant phenotype

Multidisciplinary management of locally advanced–borderline resectable adenocarcinoma of the head of the pancreas

Effectiveness of oxaliplatin desensitization protocols

Decreased expression of chromodomain helicase DNA-binding protein 5 is an unfavorable prognostic marker in patients with primary gallbladder carcinoma

Keynote webinar | Spotlight on antibody–drug conjugates in cancer