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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2021

Open Access 01-12-2021 | Escherichia Coli | Research article

Microbial yield from infectious tissues pretreated by various methods: an invitro study

Authors: Yuanqing Cai, Xinyu Fang, Lvheng Zhang, Xurong Yang, Lixiong Nie, Zida Huang, Wenbo Li, Chaofan Zhang, Bin Yang, Zhenpeng Guan, Wenming Zhang

Published in: BMC Musculoskeletal Disorders | Issue 1/2021

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Abstract

Background

This study aimed to evaluate the effects of different pretreatment methods on the microbial yield from infectious tissues.

Methods

Strains of Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used to construct single-surface, full-surface, and internal infection models in sterile pork tissue. Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), and direct culture (DC) were used to pretreat these tissues, the microbial yield from different pretreatment methods were recorded and compared. Moreover, periprosthetic tissues collected intraoperatively from periprosthetic joint infection (PJI) patients were used as a verification.

Results

The study showed that the microbial yield from MH pretreatment was significantly higher than that of MM (P < 0.01) and SF pretreatment method (P < 0.01). Furthermore, in the internal infection model, the microbial yield from MH group was also significantly higher than that of SF (P < 0.01), DTT (P < 0.01), and DC group (P < 0.01). Moreover, the number of bacterial colonies obtained from periprosthetic tissues pretreated by MH was significantly higher than pretreated by other pretreatment methods (P = 0.004).

Conclusions

The effects of MH and DTT in microbial yield were significantly higher than that of DC, SF and MM, and these methods can be used to process multiple tissue samples at the same time, which might further improve the diagnostic sensitivity of infectious disease.
Appendix
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Metadata
Title
Microbial yield from infectious tissues pretreated by various methods: an invitro study
Authors
Yuanqing Cai
Xinyu Fang
Lvheng Zhang
Xurong Yang
Lixiong Nie
Zida Huang
Wenbo Li
Chaofan Zhang
Bin Yang
Zhenpeng Guan
Wenming Zhang
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2021
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-021-04071-5

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