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Open Access 01-12-2017 | Research article

A new multiple trauma model of the mouse

Authors: Stefanie Fitschen-Oestern, Sebastian Lippross, Tim Klueter, Matthias Weuster, Deike Varoga, Mersedeh Tohidnezhad, Thomas Pufe, Stefan Rose-John, Hagen Andruszkow, Frank Hildebrand, Nadine Steubesand, Andreas Seekamp, Claudia Neunaber

Published in: BMC Musculoskeletal Disorders | Issue 1/2017

Abstract

Background

Blunt trauma is the most frequent mechanism of injury in multiple trauma, commonly resulting from road traffic collisions or falls. Two of the most frequent injuries in patients with multiple trauma are chest trauma and extremity fracture. Several trauma mouse models combine chest trauma and head injury, but no trauma mouse model to date includes the combination of long bone fractures and chest trauma. Outcome is essentially determined by the combination of these injuries. In this study, we attempted to establish a reproducible novel multiple trauma model in mice that combines blunt trauma, major injuries and simple practicability.

Methods

Ninety-six male C57BL/6 N mice (n = 8/group) were subjected to trauma for isolated femur fracture and a combination of femur fracture and chest injury. Serum samples of mice were obtained by heart puncture at defined time points of 0 h (hour), 6 h, 12 h, 24 h, 3 d (days), and 7 d.

Results

A tendency toward reduced weight and temperature was observed at 24 h after chest trauma and femur fracture. Blood analyses revealed a decrease in hemoglobin during the first 24 h after trauma. Some animals were killed by heart puncture immediately after chest contusion; these animals showed the most severe lung contusion and hemorrhage. The extent of structural lung injury varied in different mice but was evident in all animals. Representative H&E-stained (Haematoxylin and Eosin-stained) paraffin lung sections of mice with multiple trauma revealed hemorrhage and an inflammatory immune response. Plasma samples of mice with chest trauma and femur fracture showed an up-regulation of IL-1β (Interleukin-1β), IL-6, IL-10, IL-12p70 and TNF-α (Tumor necrosis factor- α) compared with the control group. Mice with femur fracture and chest trauma showed a significant up-regulation of IL-6 compared to group with isolated femur fracture.

Conclusions

The multiple trauma mouse model comprising chest trauma and femur fracture enables many analogies to clinical cases of multiple trauma in humans and demonstrates associated characteristic clinical and pathophysiological changes. This model is easy to perform, is economical and can be used for further research examining specific immunological questions.

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Title: A new multiple trauma model of the mouse
Authors: Stefanie Fitschen-Oestern
Sebastian Lippross
Tim Klueter
Matthias Weuster
Deike Varoga
Mersedeh Tohidnezhad
Thomas Pufe
Stefan Rose-John
Hagen Andruszkow
Frank Hildebrand
Nadine Steubesand
Andreas Seekamp
Claudia Neunaber
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2017
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/s12891-017-1813-9

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

A new multiple trauma model of the mouse

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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