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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2015

Open Access 01-12-2015 | Research article

Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions

Authors: Rainer Beckmann, Sebastian Lippross, Claudia Hartz, Mersedeh Tohidnezhad, Mónica S. Ventura Ferreira, Sabine Neuss-Stein, Andreas Seekamp, Sven Nebelung, Nisreen Kweider, Björn Rath, Holger Jahr, Thomas Pufe, Deike J. Varoga

Published in: BMC Musculoskeletal Disorders | Issue 1/2015

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Abstract

Background

Abrasion arthroplasty (AAP) is a procedure by which intrinsic cartilage healing is believed to be stimulated. Although clinically accepted for degenerative and traumatic cartilage lesions scientific evidence at a molecular level that proves the effect of AAP is scarce.

Method

Mononuclear cells were extracted from postoperative joint effusions 21.5 h post AAP and simple debridement of cartilage lesions. Luminex, ELISA and FACS experiments were performed. Immunohistochemical stainings of cell cultures for cartilage markers were used to confirm the findings.

Results

Postoperative joint effusions after AAP showed increased contents of Mononuclear cells compared to Arthroscopic Chondroplasty (ACP). BMP-4 and IGF were increased in AAP as complared to ACP. Mononuclear cells isolated after AAP express the MSC markers CD 73, CD 105, CD 90, CD 44 and are CD34 negative. Chondrogenic differentiation was demonstrated by positive staining for Sox9, collagen II, proteoglycan, chondroitin-4-sulfate.

Conclusion

Our results support the clinical application of AAP as a procedure that enhances cartilage repair as an alternative to far more complex procedures that have gained popularity. Furthermore the data presented supports clinical investigations that recommend not to use suction drainage as by this procedure a considerable amount of the regeneratory potential of postoperative joint effusions might be extracted.
Literature
1.
Bedi A, Feeley BT, Williams 3rd RJ. Management of articular cartilage defects of the knee. J Bone Joint Surg Am. 2010;92(4):994–1009.CrossRefPubMed
2.
Salzmann GM, Sah B, Sudkamp NP, Niemeyer P. Reoperative characteristics after microfracture of knee cartilage lesions in 454 patients. Knee Surg Sports Traumatol Arthrosc. 2013;21(2):365–71.CrossRefPubMed
3.
Matsunaga D, Akizuki S, Takizawa T, Yamazaki I, Kuraishi J. Repair of articular cartilage and clinical outcome after osteotomy with microfracture or abrasion arthroplasty for medial gonarthrosis. Knee. 2007;14(6):465–71.CrossRefPubMed
4.
Johnson LL. Arthroscopic abrasion arthroplasty: a review. Clin Orthop Relat Res. 2001;391 Suppl:S306–17.CrossRefPubMed
5.
Hunziker EB. Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthritis Cartilage. 2002;10(6):432–63.CrossRefPubMed
6.
Marlovits S, Striessnig G, Resinger CT, Aldrian SM, Vecsei V, Imhof H, et al. Definition of pertinent parameters for the evaluation of articular cartilage repair tissue with high-resolution magnetic resonance imaging. Eur J Radiol. 2004;52(3):310–9.CrossRefPubMed
7.
Ramappa AJ, Gill TJ, Bradford CH, Ho CP, Steadman JR. Magnetic resonance imaging to assess knee cartilage repair tissue after microfracture of chondral defects. J Knee Surg. 2007;20(3):228–34.PubMed
8.
Lee DH, Sonn CH, Han SB, Oh Y, Lee KM, Lee SH. Synovial fluid CD34(−) CD44(+) CD90(+) mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis. Osteoarthritis Cartilage. 2012;20(2):106–9.CrossRefPubMed
9.
Veronesi F, Maglio M, Tschon M, Aldini NN, Fini M. Adipose-derived mesenchymal stem cells for cartilage tissue engineering: state-of-the-art in in vivo studies. J Biomed Mater Res A. 2014;102(7):2448–66.CrossRefPubMed
10.
Jo CH, Lee YG, Shin WH, Kim H, Chai JW, Jeong EC, et al. Intra-articular injection of mesenchymal stem cells for the treatment of osteoarthritis of the knee: a proof-of-concept clinical trial. Stem Cells. 2014;32(5):1254–66.CrossRefPubMed
11.
Hung BP, Babalola OM, Bonassar LJ. Quantitative characterization of mesenchymal stem cell adhesion to the articular cartilage surface. J Biomed Mater Res A. 2013;101(12):3592–8.CrossRefPubMed
12.
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315–7.CrossRefPubMed
13.
Clifton R, Haleem S, McKee A, Parker MJ. Closed suction surgical wound drainage after anterior cruciate ligament reconstruction: a systematic review of randomised controlled trials. Knee. 2007;14(5):348–51.CrossRefPubMed
14.
McCormack RG, Greenhow RJ, Fogagnolo F, Shrier I. Intra-articular drain versus no drain after arthroscopic anterior cruciate ligament reconstruction: a randomized, prospective clinical trial. Arthroscopy. 2006;22(8):889–93.CrossRefPubMed
15.
Albilia JB, Tenenbaum HC, Clokie CM, Walt DR, Baker GI, Psutka DJ, et al. Serum levels of BMP-2, 4, 7 and AHSG in patients with degenerative joint disease requiring total arthroplasty of the hip and temporomandibular joints. J Orthop Res. 2013;31(1):44–52.CrossRefPubMed
16.
Kasten P, Beyen I, Egermann M, Suda AJ, Moghaddam AA, Zimmermann G, et al. Instant stem cell therapy: characterization and concentration of human mesenchymal stem cells in vitro. Eur Cell Mater. 2008;16:47–55.PubMed
17.
Kawakami Y, Tsuda M, Takahashi S, Taniguchi N, Esteban CR, Zemmyo M, et al. Transcriptional coactivator PGC-1alpha regulates chondrogenesis via association with Sox9. Proc Natl Acad Sci U S A. 2005;102(7):2414–9.CrossRefPubMedPubMedCentral
18.
de Crombrugghe B, Lefebvre V, Behringer RR, Bi W, Murakami S, Huang W. Transcriptional mechanisms of chondrocyte differentiation. Matrix Biol. 2000;19(5):389–94.CrossRefPubMed
19.
Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng. 1998;4(4):415–28.CrossRefPubMed
20.
Nakamura T, Sekiya I, Muneta T, Hatsushika D, Horie M, Tsuji K, et al. Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs. Cytotherapy. 2012;14(3):327–38.CrossRefPubMedPubMedCentral
21.
Puetzer JL, Petitte JN, Loboa EG. Comparative review of growth factors for induction of three-dimensional in vitro chondrogenesis in human mesenchymal stem cells isolated from bone marrow and adipose tissue. Tissue Eng Part B Rev. 2010;16(4):435–44.CrossRefPubMed
22.
Steinert AF, Proffen B, Kunz M, Hendrich C, Ghivizzani SC, Noth U, et al. Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer. Arthritis Res Ther. 2009;11(5):R148.CrossRefPubMedPubMedCentral
23.
Shen B, Wei A, Whittaker S, Williams LA, Tao H, Ma DD, et al. The role of BMP-7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro. J Cell Biochem. 2010;109(2):406–16.PubMed
24.
Cameron ML, Briggs KK, Steadman JR. Reproducibility and reliability of the outerbridge classification for grading chondral lesions of the knee arthroscopically. Am J Sports Med. 2003;31(1):83–6.PubMed
25.
Gobbi A, Karnatzikos G, Kumar A. Long-term results after microfracture treatment for full-thickness knee chondral lesions in athletes. Knee Surg Sports Traumatol Arthrosc. 2013.
Metadata
Title
Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions
Authors
Rainer Beckmann
Sebastian Lippross
Claudia Hartz
Mersedeh Tohidnezhad
Mónica S. Ventura Ferreira
Sabine Neuss-Stein
Andreas Seekamp
Sven Nebelung
Nisreen Kweider
Björn Rath
Holger Jahr
Thomas Pufe
Deike J. Varoga
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2015
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-015-0705-0

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