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Open Access 01-12-2015 | Research article

Screening for IgG4-type anti-nuclear antibodies in IgG4-related disease

Authors: Kazuhiro Kiyama, Hajime Yoshifuji, Tsugumitsu Kandou, Yuji Hosono, Koji Kitagori, Ran Nakashima, Yoshitaka Imura, Naoichiro Yukawa, Koichiro Ohmura, Takao Fujii, Daisuke Kawabata, Tsuneyo Mimori

Published in: BMC Musculoskeletal Disorders | Issue 1/2015

Abstract

Background

Immunoglobulin (Ig) G4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and infiltration of IgG4⁺ plasma cells into multiple organs. It is not known whether serum IgG4 is autoreactive in IgG4-RD.

Methods

We measured anti-nuclear antibody (ANA) in 19 IgG4-RD cases, determined IgG subclasses of the ANA, and compared them with those of other systemic autoimmune diseases (systemic lupus erythematosus, Sjögren’s syndrome, systemic sclerosis, and polymyositis), using subclass-based ANA test (indirect immunofluorescence).

Results

58 % of IgG4-RD cases were ANA-positive (cut-off: 1:40). Whereas their subclass of ANA was predominantly IgG2, we observed no IgG4-type ANA. In systemic autoimmune diseases, subclasses of ANA were mostly IgG1, 2, or 3, but IgG4-type ANA was very rarely detected. We also found several patients in whose serum ANA patterns differed among IgG subclasses, probably due to the difference of corresponding autoantigens.

Conclusions

Although IgG4 is highly elevated in sera of IgG4-RD patients, their ANA do not include IgG4 subclass. These results offer new insight into the role of IgG4 and the pathogenesis of IgG4-RD, implying that each IgG subclass tends to cover its own spectrum of antigens, and IgG4 is not preferentially used to make ANA.

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Title: Screening for IgG4-type anti-nuclear antibodies in IgG4-related disease
Authors: Kazuhiro Kiyama
Hajime Yoshifuji
Tsugumitsu Kandou
Yuji Hosono
Koji Kitagori
Ran Nakashima
Yoshitaka Imura
Naoichiro Yukawa
Koichiro Ohmura
Takao Fujii
Daisuke Kawabata
Tsuneyo Mimori
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2015
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/s12891-015-0584-4

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Screening for IgG4-type anti-nuclear antibodies in IgG4-related disease

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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