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BMC Pulmonary Medicine

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Open Access 01-12-2020 | Research article

Optimal specimen type for accurate diagnosis of infectious peripheral pulmonary lesions by mNGS

Authors: Qing Wang, Bo Wu, Donglin Yang, Chao Yang, Zhixian Jin, Jie Cao, Jing Feng

Published in: BMC Pulmonary Medicine | Issue 1/2020

Abstract

Background

Reports on the application of metagenomic next-generation sequencing (mNGS) to the diagnosis of peripheral pulmonary lesions (PPLs) are scarce. There have been no studies investigating the optimal specimen type for mNGS.

Methods

We used mNGS to detect pathogens in matched transbronchial lung biopsy (TBLB), bronchoalveolar lavage fluid (BALF), and bronchial needle brushing (BB) specimens from 39 patients suspected of having infectious PPLs. We explored differences in microbial composition and diagnostic accuracy of mNGS for the 3 specimen types.

Results

mNGS was more sensitive than conventional culture for detection of bacteria and fungi in TBLB, BALF, and BB specimens, with no difference in the sensitivity of mNGS across the different specimen types. mNGS showed higher sensitivity for fungi or uncategorized pulmonary pathogens in TBLB+BALF+BB compared to TBLB but not BALF or BB specimens. There were no significant differences between the 3 specimen types in the relative abundance of pathogens, or between TBLB and BB specimens in the relative abundance of 6 common lower respiratory tract commensals.

Conclusions

mNGS has a higher sensitivity than the conventional culture method for detecting pathogens in TBLB, BALF, or BB specimens. mNGS of BB samples is a less invasive alternative to TBLB for the diagnosis of infectious PPLs.

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Title: Optimal specimen type for accurate diagnosis of infectious peripheral pulmonary lesions by mNGS
Authors: Qing Wang
Bo Wu
Donglin Yang
Chao Yang
Zhixian Jin
Jie Cao
Jing Feng
Publication date: 01-12-2020
Publisher: BioMed Central
Published in: BMC Pulmonary Medicine / Issue 1/2020
Electronic ISSN: 1471-2466
DOI: https://doi.org/10.1186/s12890-020-01298-1

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