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Open Access 01-12-2018 | Research article

CC16 levels correlate with cigarette smoke exposure in bronchial epithelial cells and with lung function decline in smokers

Authors: David Chi-Leung Lam, Hoi-Hin Kwok, Wai-Cho Yu, Fanny Wai-San Ko, Cheuk-Yin Tam, Arthur Chun-Wing Lau, Daniel Yee-Tak Fong, Mary Sau-Man Ip

Published in: BMC Pulmonary Medicine | Issue 1/2018

Abstract

Background

Club cell protein-16 (CC16) expression has been associated with smoking-related lung function decline. The study hypothesis was that CC16 expression in both serum and bronchial epithelium is associated with lung function decline in smokers, and exposure to cigarette smoke will lead to reduction in CC16 expression in bronchial epithelial cells.

Methods

In a cohort of community-based male Chinese subjects recruited for lung function test in 2000, we reassessed their lung function ten years later and measured serum levels of CC16. CC16 expression was further assayed in bronchial epithelium from endobronchial biopsies taken from an independent cohort of subjects undergoing autofluorescence bronchoscopy, and tested for correlation between CC16 immunostaining intensity and lung function. In an in-vitro model, bronchial epithelial cells were exposed to cigarette smoke extract (CSE), and the expression levels of CC16 were measured in bronchial epithelial cells before and after exposure to CSE.

Results

There was a significant association between FEV₁ decline and serum CC16 levels in smokers. Expression of CC16 in bronchial epithelium showed significant correlation with FEV₁/FVC. Bronchial epithelial cells showed significant decrease in CC16 expression after exposure to CSE, followed by a subsequent rise in CC16 expression upon removal of CSE.

Conclusions

Results of these clinical and laboratory investigations suggested that low serum CC16 was associated with smoking-related decline in lung function, demonstrated the first time in a Chinese cohort. The data also lend support to the putative role of CC16 in protection against smoking-related bronchial epithelial damage.

(Abstract word count: 243)

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Title: CC16 levels correlate with cigarette smoke exposure in bronchial epithelial cells and with lung function decline in smokers
Authors: David Chi-Leung Lam
Hoi-Hin Kwok
Wai-Cho Yu
Fanny Wai-San Ko
Cheuk-Yin Tam
Arthur Chun-Wing Lau
Daniel Yee-Tak Fong
Mary Sau-Man Ip
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Pulmonary Medicine / Issue 1/2018
Electronic ISSN: 1471-2466
DOI: https://doi.org/10.1186/s12890-018-0607-7

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