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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

CTHRC1 induces non-small cell lung cancer (NSCLC) invasion through upregulating MMP-7/MMP-9

Authors: Weiling He, Hui Zhang, Yuefeng Wang, Yanbin Zhou, Yifeng Luo, Yongmei Cui, Neng Jiang, Wenting Jiang, Han Wang, Di Xu, Shuhua Li, Zhuo Wang, Yangshan Chen, Yu Sun, Yang Zhang, Hsian-Rong Tseng, Xuenong Zou, Liantang Wang, Zunfu Ke

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

The strong invasive and metastatic nature of non-small cell lung cancer (NSCLC) leads to poor prognosis. Collagen triple helix repeat containing 1 (CTHRC1) is involved in cell migration, motility and invasion. The object of this study is to investigate the involvement of CTHRC1 in NSCLC invasion and metastasis.

Methods

A proteomic analysis was performed to identify the different expression proteins between NSCLC and normal tissues. Cell lines stably express CTHRC1, MMP7, MMP9 were established. Invasion and migration were determined by scratch and transwell assays respectively. Clinical correlations of CTHRC1 in a cohort of 230 NSCLC patients were analysed.

Results

CTHRC1 is overexpressed in NSCLC as measured by proteomic analysis. Additionally, CTHRC1 increases tumour cell migration and invasion in vitro. Furthermore, CTHRC1 expression is significantly correlated with matrix metalloproteinase (MMP)7 and MMP9 expression in sera and tumour tissues from NSCLC. The invasion ability mediated by CTHRC1 were mainly MMP7- and MMP9-dependent. MMP7 or MMP9 depletion significantly eradicated the pro-invasive effects mediated by CTHRC1 on NSCLC cells. Clinically, patients with high CTHRC1 expression had poor survival.

Conclusions

CTHRC1 serves as a pro-metastatic gene that contributes to NSCLC invasion and metastasis, which are mediated by upregulated MMP7 and MMP9 expression. Targeting CTHRC1 may be beneficial for inhibiting NSCLC metastasis.
Appendix
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Metadata
Title
CTHRC1 induces non-small cell lung cancer (NSCLC) invasion through upregulating MMP-7/MMP-9
Authors
Weiling He
Hui Zhang
Yuefeng Wang
Yanbin Zhou
Yifeng Luo
Yongmei Cui
Neng Jiang
Wenting Jiang
Han Wang
Di Xu
Shuhua Li
Zhuo Wang
Yangshan Chen
Yu Sun
Yang Zhang
Hsian-Rong Tseng
Xuenong Zou
Liantang Wang
Zunfu Ke
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-4317-6

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