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BMC Nephrology
Published in: BMC Nephrology 1/2022

Open Access 01-12-2022 | Acute Kidney Injury | Research

The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise

Authors: Nazanin Sabet, Zahra Soltani, Mohammad Khaksari, Maryam Iranpour, Reza Malekpour Afshar, Fatemeh Mousavi Mehdiabadi, Alireza Raji-Amirhasani

Published in: BMC Nephrology | Issue 1/2022

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Abstract

Background

Exercise and some pre-AKI diets have been shown to improve injury, apoptosis, and lipid profile. In this study, the effect of two different diets along with exercise training on acute kidney injury (AKI) was investigated. 

Materials and methods

Laboratory rats were randomly divided into four groups of control, standard diet + exercise, exercise + calorie restriction (CR) and exercise + time restriction (TR). Each group was divided into two subgroups of AKI and no AKI. The animals received endurance training and diet regimens before AKI. Fasting blood glucose, serum creatinine, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl2) and histopathological outcome of renal tissue as well as serum lipid profile of animals were assessed 24 h after AKI. 

Results

The percentage of changes in renal Bcl2 and Bax after AKI in the group with previous exercise was lower than the group without previous exercise (p < 0.01). After induction of AKI, serum lipid profile changed in non-exercised rats (p < 0.001). Also, after injury, fasting blood glucose levels increased in non-exercised rats (p < 0.05). After injury, the start of both CR and TR diets during exercise caused less change in Bcl2 and Bax of non-exercised rats compared to exercised rats (p < 0.001). CR diet along with exercise improved lipid profile, and also CR diet along exercise decreased fasting blood glucose levels (p < 0.001). Also, both the CR and TR diets during exercise caused fewer changes in histopathological outcome after AKI.

Conclusion

Exercise alone decreased changes in apoptotic and histopathological indexes, fasting blood glucose, as well as lipid profile of rats after AKI. Reduction of apoptosis and improvement of histopathological outcome after AKI appeared more when CR and TR diets were commenced during exercise. The reduction of lipid profile changes was more pronounced in the group that received CR diet during exercise.
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Metadata
Title
The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise
Authors
Nazanin Sabet
Zahra Soltani
Mohammad Khaksari
Maryam Iranpour
Reza Malekpour Afshar
Fatemeh Mousavi Mehdiabadi
Alireza Raji-Amirhasani
Publication date
01-12-2022
Publisher
BioMed Central
Published in
BMC Nephrology / Issue 1/2022
Electronic ISSN: 1471-2369
DOI
https://doi.org/10.1186/s12882-022-02938-w

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