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Open Access 01-12-2017 | Research article

Lower genetic variability of HIV-1 and antiretroviral drug resistance in pregnant women from the state of Pará, Brazil

Authors: Luiz Fernando Almeida Machado, Iran Barros Costa, Maria Nazaré Folha, Anderson Levy Bessa da Luz, Antonio Carlos Rosário Vallinoto, Ricardo Ishak, Marluisa Oliveira Guimarães Ishak

Published in: BMC Infectious Diseases | Issue 1/2017

Abstract

Background

The present study aimed to describe the genetic diversity of HIV-1, as well as the resistance profile of the viruses identified in HIV-1 infected pregnant women under antiretroviral therapy in the state of Pará, Northern Brazil.

Methods

Blood samples were collected from 45 HIV-1 infected pregnant to determine the virus subtypes according to the HIV-1 protease (PR) gene and part of the HIV-1 reverse transcriptase (RT) gene by sequencing the nucleotides of these regions. Drug resistance mutations and susceptibility to antiretroviral drugs were analyzed by the Stanford HIV Drug Resistance Database.

Results

Out of 45 samples, only 34 could be amplified for PR and 30 for RT. Regarding the PR gene, subtypes B (97.1%) and C (2.9%) were identified; for the RT gene, subtypes B (90.0%), F (6.7%), and C (3.3%) were detected. Resistance to protease inhibitors (PI) was identified in 5.8% of the pregnant, and mutations conferring resistance to nucleoside reverse transcriptase inhibitors were found in 3.3%, while mutations conferring resistance to non-nucleoside reverse transcriptase inhibitors were found in 3.3%.

Conclusions

These results showed a low frequency of strains resistant to antiretroviral drugs, the prevalence of subtypes B and F, and the persistent low transmission of subtype C in pregnant of the state of Pará, Brazil.

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Title: Lower genetic variability of HIV-1 and antiretroviral drug resistance in pregnant women from the state of Pará, Brazil
Authors: Luiz Fernando Almeida Machado
Iran Barros Costa
Maria Nazaré Folha
Anderson Levy Bessa da Luz
Antonio Carlos Rosário Vallinoto
Ricardo Ishak
Marluisa Oliveira Guimarães Ishak
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Infectious Diseases / Issue 1/2017
Electronic ISSN: 1471-2334
DOI: https://doi.org/10.1186/s12879-017-2392-y

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Background

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Results

Conclusions

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