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Open Access 01-12-2017 | Research article

Molecular epidemiology of bla _OXA-23-producing carbapenem-resistant Acinetobacter baumannii in a single institution over a 65-month period in north China

Authors: Nian-zhi Ning, Xiong Liu, Chun-mei Bao, Su-ming Chen, En-bo Cui, Ju-ling zhang, Jie Huang, Fang-hong Chen, Tao Li, Fen Qu, Hui Wang

Published in: BMC Infectious Diseases | Issue 1/2017

Abstract

Background

Carbapenem-resistant Acinetobacter baumannii poses a significant threat to hospitalized patients, as few therapeutic options remain. Thus, we investigated the molecular epidemiology and mechanism of resistance of carbapenem-resistant A.baumannii isolates in Beijing, China.

Methods

Carbapenem-resistant A.baumannii isolates (n = 101) obtained between June 2009 and November 2014 were used. Multilocus sequence typing (MLST) and PCR assays for class C and D β-lactamase were performed on all isolates. S1 nuclease pulsed-field gel electrophoresis (PFGE) and Southern blot hybridization were performed to identify the resistance gene location.

Results

All 101 A.baumannii isolates were highly resistant to frequently used antimicrobials, and were considered multidrug resistant. A total of 12 sequence types (STs) were identified, including 10 reported STs and 2 novel STs. Eighty-seven isolates were classified to clonal complex 92 (CC92), among which ST191 and ST195 were the most common STs. The bla _OXA-23 gene was positive in most (n = 95) of the A.baumannii isolates. Using S1-nuclease digestion PFGE and Southern blot hybridization, 3 patterns of plasmids carrying bla _OXA-23 were confirmed. ST191 and ST195 (both harboring bla _OXA-23) caused outbreaks during the study period, and this is the first report of outbreaks caused by ST191 and ST195 in north China.

Conclusion

bla _OXA-23-producing A.baumannii ST191 and ST 195 isolates can disseminate in a hospital and are potential nosocomial outbreak strains. Surveillance of imipenem-resistant A.baumannii and antimicrobial stewardship should be strengthened.

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Title: Molecular epidemiology of bla OXA-23 -producing carbapenem-resistant Acinetobacter baumannii in a single institution over a 65-month period in north China
Authors: Nian-zhi Ning
Xiong Liu
Chun-mei Bao
Su-ming Chen
En-bo Cui
Ju-ling zhang
Jie Huang
Fang-hong Chen
Tao Li
Fen Qu
Hui Wang
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Infectious Diseases / Issue 1/2017
Electronic ISSN: 1471-2334
DOI: https://doi.org/10.1186/s12879-016-2110-1

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Abstract

Background

Methods

Results

Conclusion

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