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Open Access 01-12-2017 | Research article

The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP

Authors: Noriyuki Takahashi, Takayuki Saitoh, Nanami Gotoh, Yasuhiro Nitta, Lobna Alkebsi, Tetsuhiro Kasamatsu, Yusuke Minato, Akihiko Yokohama, Norifumi Tsukamoto, Hiroshi Handa, Hirokazu Murakami

Published in: BMC Immunology | Issue 1/2017

Abstract

Background

T-helper cell type 1 (Th1) polarization in chronic immune thrombocytopenia (cITP) has been reported at the protein and mRNA levels. We evaluated the impact of Th1/Th2 cytokine and cytokine receptor functional polymorphisms on both susceptibility to, and severity of, cITP. We analysed IFN-γ + 874 T/A, IFN-γR -611G/A, IL-4 -590C/T, and IL-4Rα Q576R polymorphisms in 126 cITP patients (male/female: 34/92; median age: 47.7 years) and 202 healthy control donors. Genotyping was determined by PCR and direct sequencing. The Th1/Th2 ratio was detected in peripheral blood mononuclear cells via flow cytometry.

Results

cITP patients had a higher frequency of the IL-4Rα 576 non-QQ genotype compared to healthy subjects (P = 0.04). cITP patients with the IFN-γ +874 non-AA genotype (high expression type) showed more severe thrombocytopenia than those with the AA genotype (P < 0.05). cITP patients had a significantly higher Th1/Th2 ratio than control patients (P < 0.01); this ratio was inversely correlated with platelet counts. Furthermore, patients with both IFN-γ +874 non-AA genotype (high expression type) and IFN-γR −611 non-AA genotype (high-function type) had a significantly higher Th1/Th2 ratio (P < 0.05).

Conclusions

The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP.

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Title: The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP
Authors: Noriyuki Takahashi
Takayuki Saitoh
Nanami Gotoh
Yasuhiro Nitta
Lobna Alkebsi
Tetsuhiro Kasamatsu
Yusuke Minato
Akihiko Yokohama
Norifumi Tsukamoto
Hiroshi Handa
Hirokazu Murakami
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Immunology / Issue 1/2017
Electronic ISSN: 1471-2172
DOI: https://doi.org/10.1186/s12865-017-0210-3

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