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Open Access 01-12-2017 | Research article

Poly I:C induces collective migration of HaCaT keratinocytes via IL-8

Authors: Kazuhide Takada, Shihoko Komine-Aizawa, Naoko Hirohata, Quang Duy Trinh, Atsuyoshi Nishina, Hirokazu Kimura, Satoshi Hayakawa

Published in: BMC Immunology | Issue 1/2017

Abstract

Background

Delayed wound healing reduces the quality of life (QOL) of patients. Thus, understanding the mechanism of wound healing is indispensable for better management. However, the role of innate immunity in wound healing is thus far unknown. Recently the involvement of TLR3 in wound healing has been evaluated. The systemic administration of polyriboinosinic-polyribocytidylic acid (poly I:C ; a substitute for viral dsRNA and a ligand of toll-like receptor 3), enhances wound healing in vivo. The aim of this study is to improve our understanding of the link between innate immunity and human wound healing, particularly in re-epithelialization.

Results

The present study showed that poly I:C significantly accelerated collective HaCaT cell migration in a scratch assay. Poly I:C also increased IL-8 and bFGF production, and anti-IL-8 antibodies significantly inhibited the migration caused by poly I:C. Human recombinant IL-8 also accelerated collective HaCaT cell migration. An immunofluorescence assay and enzyme-linked immunosorbent assay (ELISA) also revealed that poly I:C decreased E-cadherin protein levels and increased vimentin protein levels, and anti-IL-8 antibody reversed this effect. In contrast, nucleic/cytosolic protein ratios of Snail 1 were unchanged in all tested conditions.

Conclusion

Our findings demonstrated that poly I:C accelerated collective HaCaT cell migration via autocrine/paracrine secretions of IL-8 and the subsequent incomplete epithelial-mesenchymal transition (EMT). Our findings provide a new strategy for wound healing by regulating innate immune systems in re-epithelialization.

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Title: Poly I:C induces collective migration of HaCaT keratinocytes via IL-8
Authors: Kazuhide Takada
Shihoko Komine-Aizawa
Naoko Hirohata
Quang Duy Trinh
Atsuyoshi Nishina
Hirokazu Kimura
Satoshi Hayakawa
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Immunology / Issue 1/2017
Electronic ISSN: 1471-2172
DOI: https://doi.org/10.1186/s12865-017-0202-3

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Poly I:C induces collective migration of HaCaT keratinocytes via IL-8

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Results

Conclusion

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