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BMC Immunology
Published in: BMC Immunology 1/2016

Open Access 01-12-2016 | Research article

Recombinant ArtinM activates mast cells

Authors: Valéria Cintra Barbosa-Lorenzi, Nerry Tatiana Cecilio, Patricia Andressa de Almeida Buranello, Maria Cristina Pranchevicius, Maria Helena S. Goldman, Gabriela Pereira-da-Silva, Maria Cristina Roque-Barreira, Maria Célia Jamur, Constance Oliver

Published in: BMC Immunology | Issue 1/2016

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Abstract

Background

Mast cells are hematopoietically derived cells that play a role in inflammatory processes such as allergy, as well as in the immune response against pathogens by the selective and rapid release of preformed and lipid mediators, and the delayed release of cytokines. The native homotetrameric lectin ArtinM, a D-mannose binding lectin purified from Artocarpus heterophyllus seeds, is one of several lectins that are able to activate mast cells. Besides activating mast cells, ArtinM has been shown to affect several biological responses, including immunomodulation and acceleration of wound healing. Because of the potential pharmacological application of ArtinM, a recombinant ArtinM (rArtinM) was produced in Escherichia coli. The current study evaluated the ability of rArtinM to induce mast cell degranulation and activation.

Results

The glycan binding specificity of rArtinM was similar to that of jArtinM. rArtinM, via its CRD, was able to degranulate, releasing β-hexosaminidase and TNF-α, and to promote morphological changes on the mast cell surface. Moreover, rArtinM induced the release of the newly-synthesized mediator, IL-4. rArtinM does not have a co-stimulatory effect on the FcεRI degranulation via. The IgE-dependent mast cell activation triggered by rArtinM seems to be dependent on NFkB activation.

Conclusions

The lectin rArtinM has the ability to activate and degranulate mast cells via their CRDs. The present study indicates that rArtinM is a suitable substitute for the native form, jArtinM, and that rArtinM may serve as an important and reliable pharmacological agent.
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Metadata
Title
Recombinant ArtinM activates mast cells
Authors
Valéria Cintra Barbosa-Lorenzi
Nerry Tatiana Cecilio
Patricia Andressa de Almeida Buranello
Maria Cristina Pranchevicius
Maria Helena S. Goldman
Gabriela Pereira-da-Silva
Maria Cristina Roque-Barreira
Maria Célia Jamur
Constance Oliver
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2016
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/s12865-016-0161-0

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